Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings
Bipolaris oryzae and Gerlachia oryzae, which cause rice brown spot and leaf scald, respectively, are mainly disseminated by seeds. The aim of this study was to evaluate the potential of seeds microbiolization to reduce transmission of these pathogens to seedlings by using the bacteria DFs185 (Pseudo...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Tecnologia de Sementes
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| Series: | Journal of Seed Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000400004&lng=en&tlng=en |
| _version_ | 1819116462664581120 |
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| author | Andrea Bittencourt Moura Juliane Ludwig Aline Garske Santos Jaqueline Tavares Schafer Vanessa Nogueira Soares Bianca Obes Corrêa |
| author_facet | Andrea Bittencourt Moura Juliane Ludwig Aline Garske Santos Jaqueline Tavares Schafer Vanessa Nogueira Soares Bianca Obes Corrêa |
| author_sort | Andrea Bittencourt Moura |
| collection | DOAJ |
| description | Bipolaris oryzae and Gerlachia oryzae, which cause rice brown spot and leaf scald, respectively, are mainly disseminated by seeds. The aim of this study was to evaluate the potential of seeds microbiolization to reduce transmission of these pathogens to seedlings by using the bacteria DFs185 (Pseudomonas synxantha), DFs223 (P. fluorescens), DFs306 (unidentified) and DFs418 (Bacillus sp.). Seeds naturally infested/infected with both pathogens were immersed in suspension of these bacteria (A540 = 0.5) individually or in saline solution (control treatment). After 30 minutes of agitation at 10 ºC, 400 seeds were submitted to a sanity test through the blotter method and the isolate DFs223 was the best to reduce the incidence of B. oryzae and G. oryzae in both seed lots evaluated. Seeds treated like above were sowed in sterilized vermiculite. Seed transmission and growth promotion were recorded after 21 days of incubation in the same conditions. The isolates DFs185 and DFs306 reduced transmission of both pathogens, although the isolate DFs306 was the one wich gave the greatest growth increases. The evaluation of the in vitro antibiosis showed that isolates inhibited the mycelial growth of both pathogens, except DFs306. It is possible to affirm that these bacteria have the potential to be used as a seed treatment for seed-borne disease control. |
| first_indexed | 2024-12-22T05:17:28Z |
| format | Article |
| id | doaj.art-3cf9b37f635849d9aa858a90d662a114 |
| institution | Directory Open Access Journal |
| issn | 2317-1545 |
| language | English |
| last_indexed | 2024-12-22T05:17:28Z |
| publisher | Associação Brasileira de Tecnologia de Sementes |
| record_format | Article |
| series | Journal of Seed Science |
| spelling | doaj.art-3cf9b37f635849d9aa858a90d662a1142022-12-21T18:37:49ZengAssociação Brasileira de Tecnologia de SementesJournal of Seed Science2317-154536440741210.1590/2317-1545v36n41009S2317-15372014000400004Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlingsAndrea Bittencourt Moura0Juliane Ludwig1Aline Garske Santos2Jaqueline Tavares Schafer3Vanessa Nogueira Soares4Bianca Obes Corrêa5Universidade Federal de PelotasUniversidade Federal da Fronteira SulUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasUniversidade Federal de PelotasBipolaris oryzae and Gerlachia oryzae, which cause rice brown spot and leaf scald, respectively, are mainly disseminated by seeds. The aim of this study was to evaluate the potential of seeds microbiolization to reduce transmission of these pathogens to seedlings by using the bacteria DFs185 (Pseudomonas synxantha), DFs223 (P. fluorescens), DFs306 (unidentified) and DFs418 (Bacillus sp.). Seeds naturally infested/infected with both pathogens were immersed in suspension of these bacteria (A540 = 0.5) individually or in saline solution (control treatment). After 30 minutes of agitation at 10 ºC, 400 seeds were submitted to a sanity test through the blotter method and the isolate DFs223 was the best to reduce the incidence of B. oryzae and G. oryzae in both seed lots evaluated. Seeds treated like above were sowed in sterilized vermiculite. Seed transmission and growth promotion were recorded after 21 days of incubation in the same conditions. The isolates DFs185 and DFs306 reduced transmission of both pathogens, although the isolate DFs306 was the one wich gave the greatest growth increases. The evaluation of the in vitro antibiosis showed that isolates inhibited the mycelial growth of both pathogens, except DFs306. It is possible to affirm that these bacteria have the potential to be used as a seed treatment for seed-borne disease control.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000400004&lng=en&tlng=enbiological controlrice brown spotrice leaf scaldseed treatmentOryza sativa L |
| spellingShingle | Andrea Bittencourt Moura Juliane Ludwig Aline Garske Santos Jaqueline Tavares Schafer Vanessa Nogueira Soares Bianca Obes Corrêa Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings Journal of Seed Science biological control rice brown spot rice leaf scald seed treatment Oryza sativa L |
| title | Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings |
| title_full | Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings |
| title_fullStr | Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings |
| title_full_unstemmed | Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings |
| title_short | Biocontrol and seed transmission of Bipolaris oryzae and Gerlachia oryzae to rice seedlings |
| title_sort | biocontrol and seed transmission of bipolaris oryzae and gerlachia oryzae to rice seedlings |
| topic | biological control rice brown spot rice leaf scald seed treatment Oryza sativa L |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372014000400004&lng=en&tlng=en |
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