Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens
The present study investigated the effects of chronic intermittent ethanol exposure and withdrawal on dendritic morphology and spine density in the agranular insular and prelimbic cortices. Adult male Sprague−Dawley rats were passively exposed to vaporized ethanol (~37 mg/L; 12 h/day) or a...
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MDPI AG
2019-07-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/9/8/180 |
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| author | Madeline E. Frost Veronica L. Peterson Clark W. Bird Brian McCool Derek A. Hamilton |
| author_facet | Madeline E. Frost Veronica L. Peterson Clark W. Bird Brian McCool Derek A. Hamilton |
| author_sort | Madeline E. Frost |
| collection | DOAJ |
| description | The present study investigated the effects of chronic intermittent ethanol exposure and withdrawal on dendritic morphology and spine density in the agranular insular and prelimbic cortices. Adult male Sprague−Dawley rats were passively exposed to vaporized ethanol (~37 mg/L; 12 h/day) or air (control) for ten consecutive days. Dendritic length, branching, and spine density were quantified in layer II/III pyramidal neurons 24 hours or seven days following the final ethanol exposure. Compared to unexposed control animals there were structural alterations on neurons in the prelimbic cortex, and to a lesser extent the agranular insular cortex. The most prominent ethanol-related differences were the transient increases in dendritic length and branching in prelimbic neurons at 24 h post-cessation, and increased mushroom-shaped spines at seven days post-cessation. The results obtained in the prelimbic cortex are the opposite of those previously reported in the nucleus accumbens core (Peterson, et al. 2015), suggesting that these regions undergo distinct functional adaptations following ethanol exposure and withdrawal. |
| first_indexed | 2024-04-12T08:17:25Z |
| format | Article |
| id | doaj.art-3cfb7d30c94d491488c2824d3e1c0e31 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-04-12T08:17:25Z |
| publishDate | 2019-07-01 |
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| series | Brain Sciences |
| spelling | doaj.art-3cfb7d30c94d491488c2824d3e1c0e312022-12-22T03:40:43ZengMDPI AGBrain Sciences2076-34252019-07-019818010.3390/brainsci9080180brainsci9080180Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus AccumbensMadeline E. Frost0Veronica L. Peterson1Clark W. Bird2Brian McCool3Derek A. Hamilton4Department of Psychiatry and Behavioral Sciences, University of New Mexico, Albuquerque, NM 87131, USATeagasc Food Bioscience Research Centre, Carlow R93 XE12, IrelandDepartment of Neurosciences, University of New Mexico, Albuquerque, NM 87131, USADepartment of Physiology & Pharmacology, Wake Forest University School of Medicine, Winston-Salem, NC 27109, USADepartment of Psychology, University of New Mexico, Albuquerque, NM 87131, USAThe present study investigated the effects of chronic intermittent ethanol exposure and withdrawal on dendritic morphology and spine density in the agranular insular and prelimbic cortices. Adult male Sprague−Dawley rats were passively exposed to vaporized ethanol (~37 mg/L; 12 h/day) or air (control) for ten consecutive days. Dendritic length, branching, and spine density were quantified in layer II/III pyramidal neurons 24 hours or seven days following the final ethanol exposure. Compared to unexposed control animals there were structural alterations on neurons in the prelimbic cortex, and to a lesser extent the agranular insular cortex. The most prominent ethanol-related differences were the transient increases in dendritic length and branching in prelimbic neurons at 24 h post-cessation, and increased mushroom-shaped spines at seven days post-cessation. The results obtained in the prelimbic cortex are the opposite of those previously reported in the nucleus accumbens core (Peterson, et al. 2015), suggesting that these regions undergo distinct functional adaptations following ethanol exposure and withdrawal.https://www.mdpi.com/2076-3425/9/8/180alcoholfrontal cortexwithdrawalplasticityGolgidendritic morphologynucleus accumbenschronic intermittent ethanol exposure |
| spellingShingle | Madeline E. Frost Veronica L. Peterson Clark W. Bird Brian McCool Derek A. Hamilton Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens Brain Sciences alcohol frontal cortex withdrawal plasticity Golgi dendritic morphology nucleus accumbens chronic intermittent ethanol exposure |
| title | Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens |
| title_full | Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens |
| title_fullStr | Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens |
| title_full_unstemmed | Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens |
| title_short | Effects of Ethanol Exposure and Withdrawal on Neuronal Morphology in the Agranular Insular and Prelimbic Cortices: Relationship with Withdrawal-Related Structural Plasticity in the Nucleus Accumbens |
| title_sort | effects of ethanol exposure and withdrawal on neuronal morphology in the agranular insular and prelimbic cortices relationship with withdrawal related structural plasticity in the nucleus accumbens |
| topic | alcohol frontal cortex withdrawal plasticity Golgi dendritic morphology nucleus accumbens chronic intermittent ethanol exposure |
| url | https://www.mdpi.com/2076-3425/9/8/180 |
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