A low-cost device for cryoanesthesia of neonatal rodents
Studying the development of neural circuits in rodent models requires surgical access to the neonatal brain. Since commercially available stereotaxic and anesthetic equipment is designed for use in adults, reliable targeting of brain structures in such young animals can be challenging. Hypothermic c...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | HardwareX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S246806722300024X |
| _version_ | 1797796380324921344 |
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| author | Bradley B. Jamieson Xavier Cano-Ferrer George Konstantinou Elisa de Launoit Nicolas Renier Albane Imbert Johannes Kohl |
| author_facet | Bradley B. Jamieson Xavier Cano-Ferrer George Konstantinou Elisa de Launoit Nicolas Renier Albane Imbert Johannes Kohl |
| author_sort | Bradley B. Jamieson |
| collection | DOAJ |
| description | Studying the development of neural circuits in rodent models requires surgical access to the neonatal brain. Since commercially available stereotaxic and anesthetic equipment is designed for use in adults, reliable targeting of brain structures in such young animals can be challenging. Hypothermic cooling (cryoanesthesia) has been used as a preferred anesthesia approach in neonates. This commonly involves submerging neonates in ice, an approach that is poorly controllable. We have developed an affordable, simple to construct device – CryoPup – that allows for fast and robust cryoanesthesia of rodent pups. CryoPup consists of a microcontroller controlling a Peltier element and a heat exchanger. It is capable of both cooling and heating, thereby also functioning as a heating pad during recovery. Importantly, it has been designed for size compatibility with common stereotaxic frames. We validate CryoPup in neonatal mice, demonstrating that it allows for rapid, reliable and safe cryoanesthesia and subsequent recovery. This open-source device will facilitate future studies into the development of neural circuits in the postnatal brain. |
| first_indexed | 2024-03-13T03:32:12Z |
| format | Article |
| id | doaj.art-3cfb9996dfcb41e68d07dfd7c5f61a2d |
| institution | Directory Open Access Journal |
| issn | 2468-0672 |
| language | English |
| last_indexed | 2024-03-13T03:32:12Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj.art-3cfb9996dfcb41e68d07dfd7c5f61a2d2023-06-24T05:18:16ZengElsevierHardwareX2468-06722023-06-0114e00417A low-cost device for cryoanesthesia of neonatal rodentsBradley B. Jamieson0Xavier Cano-Ferrer1George Konstantinou2Elisa de Launoit3Nicolas Renier4Albane Imbert5Johannes Kohl6State-dependent Neural Processing Laboratory, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UKMaking STP, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UKMaking STP, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK; Corresponding authors.Sorbonne Université, Paris Brain Institute - ICM, INSERM, CNRS, AP-HP, Hôpital de la Pitié Salpêtrière, Paris, FranceSorbonne Université, Paris Brain Institute - ICM, INSERM, CNRS, AP-HP, Hôpital de la Pitié Salpêtrière, Paris, FranceMaking STP, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UKState-dependent Neural Processing Laboratory, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK; Corresponding authors.Studying the development of neural circuits in rodent models requires surgical access to the neonatal brain. Since commercially available stereotaxic and anesthetic equipment is designed for use in adults, reliable targeting of brain structures in such young animals can be challenging. Hypothermic cooling (cryoanesthesia) has been used as a preferred anesthesia approach in neonates. This commonly involves submerging neonates in ice, an approach that is poorly controllable. We have developed an affordable, simple to construct device – CryoPup – that allows for fast and robust cryoanesthesia of rodent pups. CryoPup consists of a microcontroller controlling a Peltier element and a heat exchanger. It is capable of both cooling and heating, thereby also functioning as a heating pad during recovery. Importantly, it has been designed for size compatibility with common stereotaxic frames. We validate CryoPup in neonatal mice, demonstrating that it allows for rapid, reliable and safe cryoanesthesia and subsequent recovery. This open-source device will facilitate future studies into the development of neural circuits in the postnatal brain.http://www.sciencedirect.com/science/article/pii/S246806722300024XCryoanesthesiaRodentsNeonatesPeltierStereotaxic surgeryThermoelectric device |
| spellingShingle | Bradley B. Jamieson Xavier Cano-Ferrer George Konstantinou Elisa de Launoit Nicolas Renier Albane Imbert Johannes Kohl A low-cost device for cryoanesthesia of neonatal rodents HardwareX Cryoanesthesia Rodents Neonates Peltier Stereotaxic surgery Thermoelectric device |
| title | A low-cost device for cryoanesthesia of neonatal rodents |
| title_full | A low-cost device for cryoanesthesia of neonatal rodents |
| title_fullStr | A low-cost device for cryoanesthesia of neonatal rodents |
| title_full_unstemmed | A low-cost device for cryoanesthesia of neonatal rodents |
| title_short | A low-cost device for cryoanesthesia of neonatal rodents |
| title_sort | low cost device for cryoanesthesia of neonatal rodents |
| topic | Cryoanesthesia Rodents Neonates Peltier Stereotaxic surgery Thermoelectric device |
| url | http://www.sciencedirect.com/science/article/pii/S246806722300024X |
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