Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways
Bioaugmentation is an effective strategy used to speed up the bioremediation of marine oil spills. In the present study, a highly efficient petroleum degrading bacterium (Pseudomonas aeruginosa ZS1) was applied to the bioremediation of simulated crude oil pollution in different sampling sites in the...
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| Format: | Article |
| Language: | English |
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IWA Publishing
2023-01-01
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| Series: | Water Science and Technology |
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| Online Access: | http://wst.iwaponline.com/content/87/1/228 |
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| author | Yu Gao Mengmeng Cai Ke Shi Rui Sun Suxiang Liu Qintong Li Xiaoyan Wang Wenxin Hua Yanlu Qiao Jianliang Xue Xinfeng Xiao |
| author_facet | Yu Gao Mengmeng Cai Ke Shi Rui Sun Suxiang Liu Qintong Li Xiaoyan Wang Wenxin Hua Yanlu Qiao Jianliang Xue Xinfeng Xiao |
| author_sort | Yu Gao |
| collection | DOAJ |
| description | Bioaugmentation is an effective strategy used to speed up the bioremediation of marine oil spills. In the present study, a highly efficient petroleum degrading bacterium (Pseudomonas aeruginosa ZS1) was applied to the bioremediation of simulated crude oil pollution in different sampling sites in the South China Sea. The metabolic pathways of ZS1 to degrade crude oil, the temporal dynamics of the microbial community response to crude oil contamination, and the biofortification process were investigated. The results showed that the abundance and diversity of the microbial community decreased sharply after the occurrence of crude oil contamination. The best degradation rate of crude oil, which was achieved in the samples from the sampling site N3 after the addition of ZS1 bacteria, was 50.94% at 50 days. C13 alkanes were totally oxidized by ZS1 in the 50 days. The degradation rate of solid n-alkanes (C18–C20) was about 70%. Based on the whole genome sequencing and the metabolites analysis of ZS1, we found that ZS1 degraded n-alkanes through the terminal oxidation pathway and aromatic compounds through the catechol pathway. This study provides data support for further research on biodegradation pathways of crude oil and contributes to the subsequent development of more reasonable bioremediation strategies.
HIGHLIGHTS
Pseudomonas aeruginosa ZS1 is an effective strain for bioremediation of crude oil.;
ZS1 has an advantageous position in the bioremediation process.;
Degradation pathway of n-alkanes and aromatic compound by ZS1 was revealed.; |
| first_indexed | 2024-04-10T09:37:23Z |
| format | Article |
| id | doaj.art-3cfd621af48748b996667cd2e18c44f0 |
| institution | Directory Open Access Journal |
| issn | 0273-1223 1996-9732 |
| language | English |
| last_indexed | 2024-04-10T09:37:23Z |
| publishDate | 2023-01-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Science and Technology |
| spelling | doaj.art-3cfd621af48748b996667cd2e18c44f02023-02-17T16:42:32ZengIWA PublishingWater Science and Technology0273-12231996-97322023-01-0187122823810.2166/wst.2022.406406Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathwaysYu Gao0Mengmeng Cai1Ke Shi2Rui Sun3Suxiang Liu4Qintong Li5Xiaoyan Wang6Wenxin Hua7Yanlu Qiao8Jianliang Xue9Xinfeng Xiao10 College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China Department of Applied Biosciences, Faculty of Life Sciences, Toyo University, Gunma 3740193, Japan College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China College of Safety and Environment Engineering, Shandong University of Science and Technology, Qingdao 266510, China Bioaugmentation is an effective strategy used to speed up the bioremediation of marine oil spills. In the present study, a highly efficient petroleum degrading bacterium (Pseudomonas aeruginosa ZS1) was applied to the bioremediation of simulated crude oil pollution in different sampling sites in the South China Sea. The metabolic pathways of ZS1 to degrade crude oil, the temporal dynamics of the microbial community response to crude oil contamination, and the biofortification process were investigated. The results showed that the abundance and diversity of the microbial community decreased sharply after the occurrence of crude oil contamination. The best degradation rate of crude oil, which was achieved in the samples from the sampling site N3 after the addition of ZS1 bacteria, was 50.94% at 50 days. C13 alkanes were totally oxidized by ZS1 in the 50 days. The degradation rate of solid n-alkanes (C18–C20) was about 70%. Based on the whole genome sequencing and the metabolites analysis of ZS1, we found that ZS1 degraded n-alkanes through the terminal oxidation pathway and aromatic compounds through the catechol pathway. This study provides data support for further research on biodegradation pathways of crude oil and contributes to the subsequent development of more reasonable bioremediation strategies. HIGHLIGHTS Pseudomonas aeruginosa ZS1 is an effective strain for bioremediation of crude oil.; ZS1 has an advantageous position in the bioremediation process.; Degradation pathway of n-alkanes and aromatic compound by ZS1 was revealed.;http://wst.iwaponline.com/content/87/1/228bioremediationcrude oil degradationmicrobial community structurepseudomonas aeruginosa zs1whole genome sequencing |
| spellingShingle | Yu Gao Mengmeng Cai Ke Shi Rui Sun Suxiang Liu Qintong Li Xiaoyan Wang Wenxin Hua Yanlu Qiao Jianliang Xue Xinfeng Xiao Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways Water Science and Technology bioremediation crude oil degradation microbial community structure pseudomonas aeruginosa zs1 whole genome sequencing |
| title | Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways |
| title_full | Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways |
| title_fullStr | Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways |
| title_full_unstemmed | Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways |
| title_short | Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways |
| title_sort | bioaugmentation enhance the bioremediation of marine crude oil pollution microbial communities and metabolic pathways |
| topic | bioremediation crude oil degradation microbial community structure pseudomonas aeruginosa zs1 whole genome sequencing |
| url | http://wst.iwaponline.com/content/87/1/228 |
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