Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals
In this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA) by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals. Recent findings of the new mecA homologue, mecALGA251,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Belgrade
2014-01-01
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| Series: | Veterinarski Glasnik |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2014/0350-24571402089R.pdf |
| _version_ | 1798031258055344128 |
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| author | Radosavljević V. Žutić Jadranka Pavlović Ljiljana Bošković Tamara Radanović O. Žutić M. |
| author_facet | Radosavljević V. Žutić Jadranka Pavlović Ljiljana Bošković Tamara Radanović O. Žutić M. |
| author_sort | Radosavljević V. |
| collection | DOAJ |
| description | In this work there was evaluated the method of detection of methicillin
resistant Staphylococcus aureus (MRSA) by using two molecular and three
phenotypic tests in investigation procedure of 70 strains of S.aureus
isolated from animals. Recent findings of the new mecA homologue, mecALGA251,
minimise the significance of mecA gene presence detection as a confirmation
method of methicillin resistant Staphylococcus aureus identification. For
this reason, along with multiplex PCR set of primers(165rDNK, nuc, mecA) for
detection mecA gene, there was also used multiplex PCR set of primers (spa,
mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with
simultaneous detection of luk-PV and spa gene fragments. In all 70
investigated isolates there was detected the presence of specific 16 SrDNK
fragment and nuc gene which encodes a thermostable S. aureus nuclease, while
in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using
two multiplex PCR tests. In the investigated strains there was determined
neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin
encoding gene. By application cefoxitin disk-diffusion, latex-agglutination
and two multiplex PCR tests, the identical results in identification 5
methicillin resistant out of 70 investigated S. aureus strains were obtained.
In our investigation there was determined a complete correlation between the
results of phenotypic and genotypic identification of methicillin resistant
S. aureus. [Projekat Ministarstva nauke Republike Srbije, br. TR 31079] |
| first_indexed | 2024-04-11T19:53:32Z |
| format | Article |
| id | doaj.art-3cfe99e1fa9141d194c932a94ce852b9 |
| institution | Directory Open Access Journal |
| issn | 0350-2457 2406-0771 |
| language | English |
| last_indexed | 2024-04-11T19:53:32Z |
| publishDate | 2014-01-01 |
| publisher | Faculty of Veterinary Medicine, Belgrade |
| record_format | Article |
| series | Veterinarski Glasnik |
| spelling | doaj.art-3cfe99e1fa9141d194c932a94ce852b92022-12-22T04:06:13ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712014-01-01681-2899910.2298/VETGL1402089R0350-24571402089RMethods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animalsRadosavljević V.0Žutić Jadranka1Pavlović Ljiljana2Bošković Tamara3Radanović O.4Žutić M.5Naučni Institut za veterinarstvo Srbije, BeogradNaučni Institut za veterinarstvo Srbije, BeogradInstitut za javno zdravlje Dr M. Jovanović Batut, BeogradMinistarstvo Poljoprivrede, šumarstva i vodoprivrede R. Srbije, BeogradNaučni Institut za veterinarstvo Srbije, BeogradNaučni Institut za veterinarstvo Srbije, BeogradIn this work there was evaluated the method of detection of methicillin resistant Staphylococcus aureus (MRSA) by using two molecular and three phenotypic tests in investigation procedure of 70 strains of S.aureus isolated from animals. Recent findings of the new mecA homologue, mecALGA251, minimise the significance of mecA gene presence detection as a confirmation method of methicillin resistant Staphylococcus aureus identification. For this reason, along with multiplex PCR set of primers(165rDNK, nuc, mecA) for detection mecA gene, there was also used multiplex PCR set of primers (spa, mecA, pvl, mecALGA251) for differentiation mecALGA251 from mecA, with simultaneous detection of luk-PV and spa gene fragments. In all 70 investigated isolates there was detected the presence of specific 16 SrDNK fragment and nuc gene which encodes a thermostable S. aureus nuclease, while in 5 out of 70 S. aureus isolates, there was proven mecA gene presence using two multiplex PCR tests. In the investigated strains there was determined neither mecC (mecALGA251)gene presence, nor Panton Valentine Leukocidin encoding gene. By application cefoxitin disk-diffusion, latex-agglutination and two multiplex PCR tests, the identical results in identification 5 methicillin resistant out of 70 investigated S. aureus strains were obtained. In our investigation there was determined a complete correlation between the results of phenotypic and genotypic identification of methicillin resistant S. aureus. [Projekat Ministarstva nauke Republike Srbije, br. TR 31079]http://www.doiserbia.nb.rs/img/doi/0350-2457/2014/0350-24571402089R.pdfStaphylococcus aureusMRSAcefoxitinmultiplex PCRmecALGA251 |
| spellingShingle | Radosavljević V. Žutić Jadranka Pavlović Ljiljana Bošković Tamara Radanović O. Žutić M. Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals Veterinarski Glasnik Staphylococcus aureus MRSA cefoxitin multiplex PCR mecALGA251 |
| title | Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals |
| title_full | Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals |
| title_fullStr | Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals |
| title_full_unstemmed | Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals |
| title_short | Methods of detection and typing of methicillin resistant Staphylococcus aureus isolated from animals |
| title_sort | methods of detection and typing of methicillin resistant staphylococcus aureus isolated from animals |
| topic | Staphylococcus aureus MRSA cefoxitin multiplex PCR mecALGA251 |
| url | http://www.doiserbia.nb.rs/img/doi/0350-2457/2014/0350-24571402089R.pdf |
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