Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province
Fusarium head blight is an important disease of small grains. It is mainly caused by members of the Fusarium graminearum species complex (FGSC). Barley and wheat growers spray fungicides, especially demethylation-inhibitor fungicides, to suppress the disease. The objective of this study was to exami...
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| Format: | Article |
| Language: | English |
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The Korean Society of Mycology
2022-12-01
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| Series: | 한국균학회지 |
| Subjects: | |
| Online Access: | http://www.kjmycology.or.kr/article/?num=N0320500404 |
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| author | Jiseon Baek Ju-Young Nah Mi-Jeong Lee Su-Bin Lim Jung-Hye Choi 자영 장 Theresa Lee Hyo-Won Choi Jeomsoon Kim |
| author_facet | Jiseon Baek Ju-Young Nah Mi-Jeong Lee Su-Bin Lim Jung-Hye Choi 자영 장 Theresa Lee Hyo-Won Choi Jeomsoon Kim |
| author_sort | Jiseon Baek |
| collection | DOAJ |
| description | Fusarium head blight is an important disease of small grains. It is mainly caused by members of the Fusarium graminearum species complex (FGSC). Barley and wheat growers spray fungicides, especially demethylation-inhibitor fungicides, to suppress the disease. The objective of this study was to examine the changes in the sensitivity of the FGSC population to the triazole fungicide, propiconazole. A total of 124 and 350 isolates of FGSC were obtained from barley and wheat in Jeolla Province during 2010-2016 and 2020-2021, respectively. The species identity and trichothecene chemotypes of the FGSC isolates were determined based on polymerase chain reaction assays targeting translation elongation factor 1-alpha and TRI12 genes, respectively. Sensitivity to propiconazole was determined based on the effective concentration that reduced 50% of the mycelial growth (EC50) using the agar dilution method. Of all isolates, F. asiaticum with the nivalenol chemotype was the most common (83.9% in 2010-2016 and 96.0% in 2020-2021), followed by F. asiaticum with the 3-acetyl deoxynivalenol chemotype (12.1% in 2010-2016 and 2.9% in 2020-2021). The EC50 values of the isolates collected in 2010-2016 and 2020-2021 ranged from 0.0180 to 11.0166 μg/mL and 1.3104 to 17.9587 μg/mL, respectively. The mean EC50 value of the isolates increased from 3.8648 μg/mL in 2010-2016 to 5.9635 μg/mL in 2020-2021. The baseline resistance to propiconazole was determined to be 7 μg/mL, based on the EC50 value of isolates collected in 2010-2016, and the ratio of resistant isolates increased from 9.7% in 2010-2016 to 28.6% in 2020-2021. |
| first_indexed | 2024-03-13T07:54:57Z |
| format | Article |
| id | doaj.art-3d008341a69749ddab07e8916eab0a99 |
| institution | Directory Open Access Journal |
| issn | 0253-651X 2383-5249 |
| language | English |
| last_indexed | 2024-03-13T07:54:57Z |
| publishDate | 2022-12-01 |
| publisher | The Korean Society of Mycology |
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| series | 한국균학회지 |
| spelling | doaj.art-3d008341a69749ddab07e8916eab0a992023-06-02T05:47:00ZengThe Korean Society of Mycology한국균학회지0253-651X2383-52492022-12-0150428128910.4489/KJM.20220030N0320500404Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla ProvinceJiseon Baek0Ju-Young Nah1Mi-Jeong Lee2Su-Bin Lim3Jung-Hye Choi4자영 장5Theresa Lee6Hyo-Won ChoiJeomsoon Kim7Microbial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaMicrobial Safety Division, National Institute of Agricultural Sciences, Wanju 55365, KoreaFusarium head blight is an important disease of small grains. It is mainly caused by members of the Fusarium graminearum species complex (FGSC). Barley and wheat growers spray fungicides, especially demethylation-inhibitor fungicides, to suppress the disease. The objective of this study was to examine the changes in the sensitivity of the FGSC population to the triazole fungicide, propiconazole. A total of 124 and 350 isolates of FGSC were obtained from barley and wheat in Jeolla Province during 2010-2016 and 2020-2021, respectively. The species identity and trichothecene chemotypes of the FGSC isolates were determined based on polymerase chain reaction assays targeting translation elongation factor 1-alpha and TRI12 genes, respectively. Sensitivity to propiconazole was determined based on the effective concentration that reduced 50% of the mycelial growth (EC50) using the agar dilution method. Of all isolates, F. asiaticum with the nivalenol chemotype was the most common (83.9% in 2010-2016 and 96.0% in 2020-2021), followed by F. asiaticum with the 3-acetyl deoxynivalenol chemotype (12.1% in 2010-2016 and 2.9% in 2020-2021). The EC50 values of the isolates collected in 2010-2016 and 2020-2021 ranged from 0.0180 to 11.0166 μg/mL and 1.3104 to 17.9587 μg/mL, respectively. The mean EC50 value of the isolates increased from 3.8648 μg/mL in 2010-2016 to 5.9635 μg/mL in 2020-2021. The baseline resistance to propiconazole was determined to be 7 μg/mL, based on the EC50 value of isolates collected in 2010-2016, and the ratio of resistant isolates increased from 9.7% in 2010-2016 to 28.6% in 2020-2021.http://www.kjmycology.or.kr/article/?num=N0320500404barleyfungicide sensitivityfusarium head blightpropiconazolewheat |
| spellingShingle | Jiseon Baek Ju-Young Nah Mi-Jeong Lee Su-Bin Lim Jung-Hye Choi 자영 장 Theresa Lee Hyo-Won Choi Jeomsoon Kim Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province 한국균학회지 barley fungicide sensitivity fusarium head blight propiconazole wheat |
| title | Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province |
| title_full | Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province |
| title_fullStr | Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province |
| title_full_unstemmed | Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province |
| title_short | Change in the Sensitivity to Propiconazole of Fusarium graminearum Species Complex Causing Head Blight of Barley and Wheat in Jeolla Province |
| title_sort | change in the sensitivity to propiconazole of fusarium graminearum species complex causing head blight of barley and wheat in jeolla province |
| topic | barley fungicide sensitivity fusarium head blight propiconazole wheat |
| url | http://www.kjmycology.or.kr/article/?num=N0320500404 |
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