Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach
Objective: Schisandrae Sphenantherae Fructus (SSF) is a traditional Chinese medicine used to treat coughs and pulmonary inflammatory diseases. However, the pharmacodynamic material basis and mechanisms for SSF in asthma treatment remain unclear. This study aims to screen the anti-asthmatic fraction...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.902324/full |
| _version_ | 1811273425184882688 |
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| author | Fan Li Bin Li Bin Li Jiushi Liu Jiushi Liu Xueping Wei Xueping Wei Tingyan Qiang Xinlu Mu Yumeng Wang Yumeng Wang Yaodong Qi Yaodong Qi Bengang Zhang Bengang Zhang Haitao Liu Haitao Liu Peigen Xiao Peigen Xiao |
| author_facet | Fan Li Bin Li Bin Li Jiushi Liu Jiushi Liu Xueping Wei Xueping Wei Tingyan Qiang Xinlu Mu Yumeng Wang Yumeng Wang Yaodong Qi Yaodong Qi Bengang Zhang Bengang Zhang Haitao Liu Haitao Liu Peigen Xiao Peigen Xiao |
| author_sort | Fan Li |
| collection | DOAJ |
| description | Objective: Schisandrae Sphenantherae Fructus (SSF) is a traditional Chinese medicine used to treat coughs and pulmonary inflammatory diseases. However, the pharmacodynamic material basis and mechanisms for SSF in asthma treatment remain unclear. This study aims to screen the anti-asthmatic fraction and verify the pharmacodynamic material basis, predict the potential mechanism, and verify the interaction ability between compounds and core targets.Methods: First, three fractions from SSF were compared in terms of composition, comparison, and anti-asthmatic effects. Then, the ultra-performance liquid chromatography-quadrupole/time-of-flight-mass spectrometry/mass spectrometry (UPLC-Q/TOF-MS/MS) strategy was used to identify the compounds from the active fraction, and the anti-asthmatic efficacy of the active fraction was further studied by the ovalbumin (OVA)-induced asthma murine model. Finally, network pharmacology and molecular methods were used to study the relationships between active compounds, core targets, and key pathways of PEF in asthma treatments.Results: The petroleum ether fraction (PEF) of SSF showed better effects and could significantly diminish lung inflammation and mitigate the level of serum immunoglobulin E (IgE), interleukin (IL)-4, IL-5, IL-6, IL-13, and IL-17 in mice. A total of 26 compounds from the PEF were identified, among which the main compounds are lignans and triterpenes. Moreover, 21 active compounds, 129 overlap-ping targets, and 10 pathways were screened by network pharmacology tools. The top five core targets may play a great role in asthma treatment. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that the PEF can treat asthma by acting on multiple asthma pathological processes, including the IL-17 signaling pathway, T helper (Th) 17 cell differentiation, and the calcium signaling pathway. Molecular docking was performed to evaluate the interactions of the protein–ligand binding, and most docked complexes had a good binding ability.Conclusion: The present results might contribute to exploring the active compounds with anti-asthmatic activity. |
| first_indexed | 2024-04-12T22:58:55Z |
| format | Article |
| id | doaj.art-3d01eb4e5dfb43f9b2380f71dc284154 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T22:58:55Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-3d01eb4e5dfb43f9b2380f71dc2841542022-12-22T03:13:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-09-011310.3389/fphar.2022.902324902324Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approachFan Li0Bin Li1Bin Li2Jiushi Liu3Jiushi Liu4Xueping Wei5Xueping Wei6Tingyan Qiang7Xinlu Mu8Yumeng Wang9Yumeng Wang10Yaodong Qi11Yaodong Qi12Bengang Zhang13Bengang Zhang14Haitao Liu15Haitao Liu16Peigen Xiao17Peigen Xiao18Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaKey Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaEngineering Research Center of Traditional Chinese Medicine Resource, Peking Union Medical College, Institute of Medicinal Plant Development, Ministry of Education, Chinese Academy of Medical Sciences, Beijing, ChinaObjective: Schisandrae Sphenantherae Fructus (SSF) is a traditional Chinese medicine used to treat coughs and pulmonary inflammatory diseases. However, the pharmacodynamic material basis and mechanisms for SSF in asthma treatment remain unclear. This study aims to screen the anti-asthmatic fraction and verify the pharmacodynamic material basis, predict the potential mechanism, and verify the interaction ability between compounds and core targets.Methods: First, three fractions from SSF were compared in terms of composition, comparison, and anti-asthmatic effects. Then, the ultra-performance liquid chromatography-quadrupole/time-of-flight-mass spectrometry/mass spectrometry (UPLC-Q/TOF-MS/MS) strategy was used to identify the compounds from the active fraction, and the anti-asthmatic efficacy of the active fraction was further studied by the ovalbumin (OVA)-induced asthma murine model. Finally, network pharmacology and molecular methods were used to study the relationships between active compounds, core targets, and key pathways of PEF in asthma treatments.Results: The petroleum ether fraction (PEF) of SSF showed better effects and could significantly diminish lung inflammation and mitigate the level of serum immunoglobulin E (IgE), interleukin (IL)-4, IL-5, IL-6, IL-13, and IL-17 in mice. A total of 26 compounds from the PEF were identified, among which the main compounds are lignans and triterpenes. Moreover, 21 active compounds, 129 overlap-ping targets, and 10 pathways were screened by network pharmacology tools. The top five core targets may play a great role in asthma treatment. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that the PEF can treat asthma by acting on multiple asthma pathological processes, including the IL-17 signaling pathway, T helper (Th) 17 cell differentiation, and the calcium signaling pathway. Molecular docking was performed to evaluate the interactions of the protein–ligand binding, and most docked complexes had a good binding ability.Conclusion: The present results might contribute to exploring the active compounds with anti-asthmatic activity.https://www.frontiersin.org/articles/10.3389/fphar.2022.902324/fullactive fraction screeninganti-asthmamolecular dockingnetwork pharmacologySchisandrae Sphenantherae FructusUPLC-Q/TOF-MS/MS |
| spellingShingle | Fan Li Bin Li Bin Li Jiushi Liu Jiushi Liu Xueping Wei Xueping Wei Tingyan Qiang Xinlu Mu Yumeng Wang Yumeng Wang Yaodong Qi Yaodong Qi Bengang Zhang Bengang Zhang Haitao Liu Haitao Liu Peigen Xiao Peigen Xiao Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach Frontiers in Pharmacology active fraction screening anti-asthma molecular docking network pharmacology Schisandrae Sphenantherae Fructus UPLC-Q/TOF-MS/MS |
| title | Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach |
| title_full | Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach |
| title_fullStr | Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach |
| title_full_unstemmed | Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach |
| title_short | Anti-asthmatic fraction screening and mechanisms prediction of Schisandrae Sphenantherae Fructus based on a combined approach |
| title_sort | anti asthmatic fraction screening and mechanisms prediction of schisandrae sphenantherae fructus based on a combined approach |
| topic | active fraction screening anti-asthma molecular docking network pharmacology Schisandrae Sphenantherae Fructus UPLC-Q/TOF-MS/MS |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.902324/full |
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