Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes
Abstract Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used to study host-microbe interactions and identify microbial taxa and assemblages...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40168-022-01256-5 |
| _version_ | 1818022535062618112 |
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| author | Kerri L. Coon Shivanand Hegde Grant L. Hughes |
| author_facet | Kerri L. Coon Shivanand Hegde Grant L. Hughes |
| author_sort | Kerri L. Coon |
| collection | DOAJ |
| description | Abstract Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used to study host-microbe interactions and identify microbial taxa and assemblages associated with health or disease. However, no such approaches have been developed to manipulate the microbiota of mosquitoes. Results Here, we developed an approach to transfer entire microbial communities between mosquito cohorts. We undertook transfers between (Culex quinquefasciatus to Aedes aegypti) and within (Ae. aegypti to Ae. aegypti) species to validate the approach and determine the number of mosquitoes required to prepare donor microbiota. After the transfer, we monitored mosquito development and microbiota dynamics throughout the life cycle. Typical holometabolous lifestyle-related microbiota structures were observed, with higher dynamics of microbial structures in larval stages, including the larval water, and less diversity in adults. Microbiota diversity in recipient adults was also more similar to the microbiota diversity in donor adults. Conclusions This study provides the first evidence for successful microbiome transplantation in mosquitoes. Our results highlight the value of such methods for studying mosquito-microbe interactions and lay the foundation for future studies to elucidate the factors underlying microbiota acquisition, assembly, and function in mosquitoes under controlled conditions. Video Abstract |
| first_indexed | 2024-12-10T03:29:58Z |
| format | Article |
| id | doaj.art-3d0395b1c0dc4ed889b722ac6779ae1c |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-12-10T03:29:58Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-3d0395b1c0dc4ed889b722ac6779ae1c2022-12-22T02:03:51ZengBMCMicrobiome2049-26182022-04-0110111310.1186/s40168-022-01256-5Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoesKerri L. Coon0Shivanand Hegde1Grant L. Hughes2Department of Bacteriology, University of Wisconsin-MadisonDepartments of Vector Biology and Tropical Disease Biology, Centre for Neglected Topical Disease, Liverpool School of Tropical MedicineDepartments of Vector Biology and Tropical Disease Biology, Centre for Neglected Topical Disease, Liverpool School of Tropical MedicineAbstract Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used to study host-microbe interactions and identify microbial taxa and assemblages associated with health or disease. However, no such approaches have been developed to manipulate the microbiota of mosquitoes. Results Here, we developed an approach to transfer entire microbial communities between mosquito cohorts. We undertook transfers between (Culex quinquefasciatus to Aedes aegypti) and within (Ae. aegypti to Ae. aegypti) species to validate the approach and determine the number of mosquitoes required to prepare donor microbiota. After the transfer, we monitored mosquito development and microbiota dynamics throughout the life cycle. Typical holometabolous lifestyle-related microbiota structures were observed, with higher dynamics of microbial structures in larval stages, including the larval water, and less diversity in adults. Microbiota diversity in recipient adults was also more similar to the microbiota diversity in donor adults. Conclusions This study provides the first evidence for successful microbiome transplantation in mosquitoes. Our results highlight the value of such methods for studying mosquito-microbe interactions and lay the foundation for future studies to elucidate the factors underlying microbiota acquisition, assembly, and function in mosquitoes under controlled conditions. Video Abstracthttps://doi.org/10.1186/s40168-022-01256-5MicrobiotaMicrobiomeTransplantationAedes aegyptiCulex quinquefasciatusInsect |
| spellingShingle | Kerri L. Coon Shivanand Hegde Grant L. Hughes Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes Microbiome Microbiota Microbiome Transplantation Aedes aegypti Culex quinquefasciatus Insect |
| title | Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes |
| title_full | Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes |
| title_fullStr | Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes |
| title_full_unstemmed | Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes |
| title_short | Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes |
| title_sort | interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes |
| topic | Microbiota Microbiome Transplantation Aedes aegypti Culex quinquefasciatus Insect |
| url | https://doi.org/10.1186/s40168-022-01256-5 |
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