Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat

Root knot nematodes (RKN) Meloidogyne spp. is the primary parasite of celery and reported to cause losses up to 70%. Identification of the Meloidogyne species on celery in Indonesia has not been reported. This study was aimed to identify the species of Meloidogyne on celery based on morphology char...

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Main Authors: Fitrianingrum Kurniawati, Supramana Supramana, Abdul Muin Adnan
Format: Article
Language:Indonesian
Published: Perhimpunan Fitopatologi Indonesia 2017-05-01
Series:Jurnal Fitopatologi Indonesia
Subjects:
Online Access:https://journal.ipb.ac.id/index.php/jfiti/article/view/16361
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author Fitrianingrum Kurniawati
Supramana Supramana
Abdul Muin Adnan
author_facet Fitrianingrum Kurniawati
Supramana Supramana
Abdul Muin Adnan
author_sort Fitrianingrum Kurniawati
collection DOAJ
description Root knot nematodes (RKN) Meloidogyne spp. is the primary parasite of celery and reported to cause losses up to 70%. Identification of the Meloidogyne species on celery in Indonesia has not been reported. This study was aimed to identify the species of Meloidogyne on celery based on morphology characters. Samples of Meloidogyne-infected celery plants were taken using purposive sampling method from Ciputri Village, District of Pacet, Cianjur, West Java Province. RKN inside root tissue was detected by acid fuchsin staining method. Meloidogyne species identification was done by morphological observation of female perineal pattern. Disease symptoms found in the field include leaf yellowing, stunted, and uneven growth of celery plants. Roots of infected plants showed the formation of small size root knots, in large numbers and forming strands like a chain. Staining NPA in root tissue was successfully detected various stages of nematode development, i.e. eggs, juveniles and female nematodes. Three Meloidogyne species, namely M. incognita, M. arenaria and M. javanica were identified.
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spelling doaj.art-3d06617d30744ab4a6276e1927fde4952023-01-04T02:43:56ZindPerhimpunan Fitopatologi IndonesiaJurnal Fitopatologi Indonesia0215-79502339-24792017-05-0113110.14692/jfi.13.1.2611889Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa BaratFitrianingrum Kurniawati0Supramana Supramana1Abdul Muin Adnan2Institut Pertanian BogorInstitut Pertanian BogorInstitut Pertanian Bogor Root knot nematodes (RKN) Meloidogyne spp. is the primary parasite of celery and reported to cause losses up to 70%. Identification of the Meloidogyne species on celery in Indonesia has not been reported. This study was aimed to identify the species of Meloidogyne on celery based on morphology characters. Samples of Meloidogyne-infected celery plants were taken using purposive sampling method from Ciputri Village, District of Pacet, Cianjur, West Java Province. RKN inside root tissue was detected by acid fuchsin staining method. Meloidogyne species identification was done by morphological observation of female perineal pattern. Disease symptoms found in the field include leaf yellowing, stunted, and uneven growth of celery plants. Roots of infected plants showed the formation of small size root knots, in large numbers and forming strands like a chain. Staining NPA in root tissue was successfully detected various stages of nematode development, i.e. eggs, juveniles and female nematodes. Three Meloidogyne species, namely M. incognita, M. arenaria and M. javanica were identified. https://journal.ipb.ac.id/index.php/jfiti/article/view/16361acid fuchsin staining methodfemale perineal patternmorphological identificationroot tissue
spellingShingle Fitrianingrum Kurniawati
Supramana Supramana
Abdul Muin Adnan
Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
Jurnal Fitopatologi Indonesia
acid fuchsin staining method
female perineal pattern
morphological identification
root tissue
title Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
title_full Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
title_fullStr Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
title_full_unstemmed Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
title_short Spesies Meloidogyne Penyebab Puru Akar pada Seledri di Pacet, Cianjur, Jawa Barat
title_sort spesies meloidogyne penyebab puru akar pada seledri di pacet cianjur jawa barat
topic acid fuchsin staining method
female perineal pattern
morphological identification
root tissue
url https://journal.ipb.ac.id/index.php/jfiti/article/view/16361
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AT supramanasupramana spesiesmeloidogynepenyebabpuruakarpadaseledridipacetcianjurjawabarat
AT abdulmuinadnan spesiesmeloidogynepenyebabpuruakarpadaseledridipacetcianjurjawabarat