High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells
A series of calcium carbonate particles with sizes of 500 ± 80 and 200 ± 90 nm were obtained using mass crystallization in aqueous salt solutions by varying the reaction conditions and adding glycerol or a combination of polyethylene glycol, polysorbat-80 and cell cultural medium to the reaction vol...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2673-9992/14/1/87 |
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| author | Tatiana Pallaeva Alexander Mikheev Daniil Eurov Dmitry Kurdyukov Victoriya Popova Elena Dmitrienko Roman Akasov Daria Trushina |
| author_facet | Tatiana Pallaeva Alexander Mikheev Daniil Eurov Dmitry Kurdyukov Victoriya Popova Elena Dmitrienko Roman Akasov Daria Trushina |
| author_sort | Tatiana Pallaeva |
| collection | DOAJ |
| description | A series of calcium carbonate particles with sizes of 500 ± 80 and 200 ± 90 nm were obtained using mass crystallization in aqueous salt solutions by varying the reaction conditions and adding glycerol or a combination of polyethylene glycol, polysorbat-80 and cell cultural medium to the reaction volume. Calcium carbonate nanoparticles of 50 ± 30 nm in diameter were synthesized within the pores of mesoporous silica particles with a subsequent etching out of the template material. A complete characterization of the particles was carried out using scanning and transmission electron microscopy, X-ray powder diffraction, and dynamic and electrophoretic light scattering. CaCO<sub>3</sub> particles were loaded with anticancer drugs, porphyrazine and doxorubicin, with an encapsulation efficiency of 2–5 and 4–11 wt.%, respectively. The spontaneous release at pH 7 reached 15%, and when the particles are dissolved at pH 4, the release was about 45% of the substance during the day, regardless of the encapsulated substance. Functionalization of the surface of calcium carbonate particles with a biocompatible Pluronic-folic acid conjugate did not affect the particle size distribution and aggregative stability for all three samples. The effect of coatings on the rate of internalization and accumulation of particles by cells expressing folic acid receptors was established. It was also shown that the internalization of 50 ± 30 nm particles was more active than other samples. |
| first_indexed | 2024-03-11T06:06:00Z |
| format | Article |
| id | doaj.art-3d06e04cb1dc4d79a1a238fbf67420ab |
| institution | Directory Open Access Journal |
| issn | 2673-9992 |
| language | English |
| last_indexed | 2024-03-11T06:06:00Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medical Sciences Forum |
| spelling | doaj.art-3d06e04cb1dc4d79a1a238fbf67420ab2023-11-17T12:57:59ZengMDPI AGMedical Sciences Forum2673-99922022-11-011418710.3390/ECMC2022-13496High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with CellsTatiana Pallaeva0Alexander Mikheev1Daniil Eurov2Dmitry Kurdyukov3Victoriya Popova4Elena Dmitrienko5Roman Akasov6Daria Trushina7A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 119333, RussiaA.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 119333, RussiaIoffe Institute, St. Petersburg 194021, RussiaIoffe Institute, St. Petersburg 194021, RussiaInstitute of Chemical Biology and Fundamental Medicine (ICBFM), Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Chemical Biology and Fundamental Medicine (ICBFM), Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Molecular Theranostics, I.M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaA.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, Moscow 119333, RussiaA series of calcium carbonate particles with sizes of 500 ± 80 and 200 ± 90 nm were obtained using mass crystallization in aqueous salt solutions by varying the reaction conditions and adding glycerol or a combination of polyethylene glycol, polysorbat-80 and cell cultural medium to the reaction volume. Calcium carbonate nanoparticles of 50 ± 30 nm in diameter were synthesized within the pores of mesoporous silica particles with a subsequent etching out of the template material. A complete characterization of the particles was carried out using scanning and transmission electron microscopy, X-ray powder diffraction, and dynamic and electrophoretic light scattering. CaCO<sub>3</sub> particles were loaded with anticancer drugs, porphyrazine and doxorubicin, with an encapsulation efficiency of 2–5 and 4–11 wt.%, respectively. The spontaneous release at pH 7 reached 15%, and when the particles are dissolved at pH 4, the release was about 45% of the substance during the day, regardless of the encapsulated substance. Functionalization of the surface of calcium carbonate particles with a biocompatible Pluronic-folic acid conjugate did not affect the particle size distribution and aggregative stability for all three samples. The effect of coatings on the rate of internalization and accumulation of particles by cells expressing folic acid receptors was established. It was also shown that the internalization of 50 ± 30 nm particles was more active than other samples.https://www.mdpi.com/2673-9992/14/1/87calcium carbonate nanoparticleshigh-capacity containersporphyrazinedoxorubicintargeted drug delivery |
| spellingShingle | Tatiana Pallaeva Alexander Mikheev Daniil Eurov Dmitry Kurdyukov Victoriya Popova Elena Dmitrienko Roman Akasov Daria Trushina High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells Medical Sciences Forum calcium carbonate nanoparticles high-capacity containers porphyrazine doxorubicin targeted drug delivery |
| title | High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells |
| title_full | High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells |
| title_fullStr | High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells |
| title_full_unstemmed | High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells |
| title_short | High-Capacity CaCO<sub>3</sub> Containers: The Effect of Size on Drug Loading and Interaction with Cells |
| title_sort | high capacity caco sub 3 sub containers the effect of size on drug loading and interaction with cells |
| topic | calcium carbonate nanoparticles high-capacity containers porphyrazine doxorubicin targeted drug delivery |
| url | https://www.mdpi.com/2673-9992/14/1/87 |
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