Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects
As angiogenesis and vasculogenesis involve the complex network structures of various types of cells, extracellular matrix components, and cytokines, it is still difficult to exactly mimic the microenvironment of vascularization in vivo. In our study, we constructed a complex containing highly prolif...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2016-02-01
|
| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368915X687930 |
| _version_ | 1818257714947555328 |
|---|---|
| author | Yun-Qing Zhang Shi-Zhao Ji He Fang Yong-Jun Zheng Peng-Fei Luo Hai-Bin Wu Min-Juan Wu Zhi-Hong Wang Shi-Chu Xiao Zhao-Fan Xia |
| author_facet | Yun-Qing Zhang Shi-Zhao Ji He Fang Yong-Jun Zheng Peng-Fei Luo Hai-Bin Wu Min-Juan Wu Zhi-Hong Wang Shi-Chu Xiao Zhao-Fan Xia |
| author_sort | Yun-Qing Zhang |
| collection | DOAJ |
| description | As angiogenesis and vasculogenesis involve the complex network structures of various types of cells, extracellular matrix components, and cytokines, it is still difficult to exactly mimic the microenvironment of vascularization in vivo. In our study, we constructed a complex containing highly proliferative fibroblasts that can secrete extracellular matrix components and growth factors to chemotaxize endothelial progenitor cells (EPCs) in an attempt to create an ideal microenvironment for quick vascularization. Amniotic membrane microparticles (mAM) rich in type IV collagen (COL IV) and laminin (LN) were prepared, and human dermal fibroblasts (HDF) were infected with lentivirus (LV) of overexpression of SDF-1α to construct SDF-1α ov HDF. Using the rotary cell culture system (RCCS), mAM was loaded with HDF or SDF-1α ov HDF to construct HDF-mAM and SDF-1α ov HDF-mAM complexes. The complexes were able to secrete various types of active peptides (IL-6, IL-8, TGF-β, and bFGF) during in vitro culture. In addition, SDF-1α ov HDF-mAM complex highly expressed SDF-1α. Transwell assay showed SDF-1α ov HDF-mAM complex had an apparent chemotactic effect on EPCs. Transplantation of complexes onto full-thickness skin defects of C57BL mice further demonstrated that SDF-1α expression and the number of peripheral EPCs at days 3, 5, and 7 in the SDF-1α ov HDF-mAM group were significantly higher than that in other groups ( p < 0.01). The local microvascular density at day 10 of transplantation showed that the microvascular density in the SDF-1α ov HDF-mAM group was significantly higher than that in HDF-mAM group ( p < 0.01). In conclusion, HDF-mAM had a strong proliferative activity and could be used to create a sound microenvironment for quick vascularization by secreting multiple cytokines and extracellular matrix components. Overexpression of SDF-1α could chemotaxize EPCs to reach local wounds, thus further accelerating angiogenesis in the transplant site. The technique described may prove to be a new model for accelerating vascularization of tissue and organ transplants and chronic ischemic wounds. |
| first_indexed | 2024-12-12T17:48:03Z |
| format | Article |
| id | doaj.art-3d09dc6d27a74d018731760df04d34c7 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T17:48:03Z |
| publishDate | 2016-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-3d09dc6d27a74d018731760df04d34c72022-12-22T00:16:53ZengSAGE PublishingCell Transplantation0963-68971555-38922016-02-012510.3727/096368915X687930Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin DefectsYun-Qing Zhang0Shi-Zhao Ji1He Fang2Yong-Jun Zheng3Peng-Fei Luo4Hai-Bin Wu5Min-Juan Wu6Zhi-Hong Wang7Shi-Chu Xiao8Zhao-Fan Xia9 Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Department of Histology and Embryology, College of Basic Medical Science, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of China Burns Institute of People's Liberation Army, Changhai Hospital, the Second Military Medical University, Shanghai, People's Republic of ChinaAs angiogenesis and vasculogenesis involve the complex network structures of various types of cells, extracellular matrix components, and cytokines, it is still difficult to exactly mimic the microenvironment of vascularization in vivo. In our study, we constructed a complex containing highly proliferative fibroblasts that can secrete extracellular matrix components and growth factors to chemotaxize endothelial progenitor cells (EPCs) in an attempt to create an ideal microenvironment for quick vascularization. Amniotic membrane microparticles (mAM) rich in type IV collagen (COL IV) and laminin (LN) were prepared, and human dermal fibroblasts (HDF) were infected with lentivirus (LV) of overexpression of SDF-1α to construct SDF-1α ov HDF. Using the rotary cell culture system (RCCS), mAM was loaded with HDF or SDF-1α ov HDF to construct HDF-mAM and SDF-1α ov HDF-mAM complexes. The complexes were able to secrete various types of active peptides (IL-6, IL-8, TGF-β, and bFGF) during in vitro culture. In addition, SDF-1α ov HDF-mAM complex highly expressed SDF-1α. Transwell assay showed SDF-1α ov HDF-mAM complex had an apparent chemotactic effect on EPCs. Transplantation of complexes onto full-thickness skin defects of C57BL mice further demonstrated that SDF-1α expression and the number of peripheral EPCs at days 3, 5, and 7 in the SDF-1α ov HDF-mAM group were significantly higher than that in other groups ( p < 0.01). The local microvascular density at day 10 of transplantation showed that the microvascular density in the SDF-1α ov HDF-mAM group was significantly higher than that in HDF-mAM group ( p < 0.01). In conclusion, HDF-mAM had a strong proliferative activity and could be used to create a sound microenvironment for quick vascularization by secreting multiple cytokines and extracellular matrix components. Overexpression of SDF-1α could chemotaxize EPCs to reach local wounds, thus further accelerating angiogenesis in the transplant site. The technique described may prove to be a new model for accelerating vascularization of tissue and organ transplants and chronic ischemic wounds.https://doi.org/10.3727/096368915X687930 |
| spellingShingle | Yun-Qing Zhang Shi-Zhao Ji He Fang Yong-Jun Zheng Peng-Fei Luo Hai-Bin Wu Min-Juan Wu Zhi-Hong Wang Shi-Chu Xiao Zhao-Fan Xia Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects Cell Transplantation |
| title | Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects |
| title_full | Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects |
| title_fullStr | Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects |
| title_full_unstemmed | Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects |
| title_short | Use of Amniotic Microparticles Coated with Fibroblasts Overexpressing SDF-1a to Create an Environment Conducive to Neovascularization for Repair of Full-Thickness Skin Defects |
| title_sort | use of amniotic microparticles coated with fibroblasts overexpressing sdf 1a to create an environment conducive to neovascularization for repair of full thickness skin defects |
| url | https://doi.org/10.3727/096368915X687930 |
| work_keys_str_mv | AT yunqingzhang useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT shizhaoji useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT hefang useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT yongjunzheng useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT pengfeiluo useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT haibinwu useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT minjuanwu useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT zhihongwang useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT shichuxiao useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects AT zhaofanxia useofamnioticmicroparticlescoatedwithfibroblastsoverexpressingsdf1atocreateanenvironmentconducivetoneovascularizationforrepairoffullthicknessskindefects |