Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production
Thailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from agricultural activities. Conventional disposal methods such as incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2023-01-01
|
| Series: | E3S Web of Conferences |
| Subjects: | |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/65/e3sconf_ri2c2023_01008.pdf |
| _version_ | 1797673503147687936 |
|---|---|
| author | Panyarachun Danipa Panakkal Elizabeth Jayex Tawai Atthasit Rodiahwati Wawat Paramasivam Baranitharan Asavasanti Suvaluk Cheenkachorn Kraipat |
| author_facet | Panyarachun Danipa Panakkal Elizabeth Jayex Tawai Atthasit Rodiahwati Wawat Paramasivam Baranitharan Asavasanti Suvaluk Cheenkachorn Kraipat |
| author_sort | Panyarachun Danipa |
| collection | DOAJ |
| description | Thailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from agricultural activities. Conventional disposal methods such as incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization of this biomass waste has been a solution. This study focuses on the utilization of Napier grass as a renewable energy source. In this experiment, the Napier grass samples were pretreated using oxalic acid with temperature variations (50 – 100 °C), time (30 180 min), and oxalic acid concentration (2 10%w/v) to determine the limit of these three factors for optimization studies. The utilization of Box-Behnken Design (BBD) within Response Surface Methodology (RSM) enabled the determination of optimal pretreatment conditions and the exploration of the correlation between pretreatment factors and reducing sugar content. The model predicted pretreatment with an oxalic acid concentration of 6% w/v, pretreated at 100 °C for 105 min as the optimal pretreatment condition to produce a maximum reducing sugar concentration of 10.65 mg/ml. Therefore, the sample was pretreated at optimum conditions and the results revealed the amount of reducing sugar obtained was 10.67 mg/ml, which differed from the predicted value with an error of 0.22%. Thus, this study provides insight for future researchers on the optimum condition that can be applied for pretreating biomass with oxalic acid to maximize the sugar yield. |
| first_indexed | 2024-03-11T21:46:22Z |
| format | Article |
| id | doaj.art-3d0c1a519d7a46589e71f74c5f678750 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-11T21:46:22Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-3d0c1a519d7a46589e71f74c5f6787502023-09-26T10:12:06ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014280100810.1051/e3sconf/202342801008e3sconf_ri2c2023_01008Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel ProductionPanyarachun Danipa0Panakkal Elizabeth Jayex1Tawai Atthasit2Rodiahwati Wawat3Paramasivam Baranitharan4Asavasanti Suvaluk5Cheenkachorn Kraipat6Department of Chemical Engineering, Faculty of Engineering, King Mongkut’s University of Technology North BangkokBiorefinery and Process Automation Engineering Center, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North BangkokBiorefinery and Process Automation Engineering Center, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut’s University of Technology North BangkokDepartment of Chemistry, University of New England:Department of Mechanical Engineering, Saveetha School of Engineering, SIMATSFood Technology & Engineering Laboratory, Pilot Plant Development & Training Institute, King Mongkut’s University of Technology ThonburiDepartment of Chemical Engineering, Faculty of Engineering, King Mongkut’s University of Technology North BangkokThailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from agricultural activities. Conventional disposal methods such as incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization of this biomass waste has been a solution. This study focuses on the utilization of Napier grass as a renewable energy source. In this experiment, the Napier grass samples were pretreated using oxalic acid with temperature variations (50 – 100 °C), time (30 180 min), and oxalic acid concentration (2 10%w/v) to determine the limit of these three factors for optimization studies. The utilization of Box-Behnken Design (BBD) within Response Surface Methodology (RSM) enabled the determination of optimal pretreatment conditions and the exploration of the correlation between pretreatment factors and reducing sugar content. The model predicted pretreatment with an oxalic acid concentration of 6% w/v, pretreated at 100 °C for 105 min as the optimal pretreatment condition to produce a maximum reducing sugar concentration of 10.65 mg/ml. Therefore, the sample was pretreated at optimum conditions and the results revealed the amount of reducing sugar obtained was 10.67 mg/ml, which differed from the predicted value with an error of 0.22%. Thus, this study provides insight for future researchers on the optimum condition that can be applied for pretreating biomass with oxalic acid to maximize the sugar yield.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/65/e3sconf_ri2c2023_01008.pdfbioethanolbiorefineryenzymatic hydrolysisorganic acidoxalic acidpretreatment |
| spellingShingle | Panyarachun Danipa Panakkal Elizabeth Jayex Tawai Atthasit Rodiahwati Wawat Paramasivam Baranitharan Asavasanti Suvaluk Cheenkachorn Kraipat Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production E3S Web of Conferences bioethanol biorefinery enzymatic hydrolysis organic acid oxalic acid pretreatment |
| title | Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production |
| title_full | Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production |
| title_fullStr | Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production |
| title_full_unstemmed | Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production |
| title_short | Oxalic Acid Pretreatment on Enhancement of Enzymatic Saccharification from Napier Grass for Biofuel Production |
| title_sort | oxalic acid pretreatment on enhancement of enzymatic saccharification from napier grass for biofuel production |
| topic | bioethanol biorefinery enzymatic hydrolysis organic acid oxalic acid pretreatment |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/65/e3sconf_ri2c2023_01008.pdf |
| work_keys_str_mv | AT panyarachundanipa oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction AT panakkalelizabethjayex oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction AT tawaiatthasit oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction AT rodiahwatiwawat oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction AT paramasivambaranitharan oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction AT asavasantisuvaluk oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction AT cheenkachornkraipat oxalicacidpretreatmentonenhancementofenzymaticsaccharificationfromnapiergrassforbiofuelproduction |