Clinicomycological Study of Dermatophytosis in South India
Introduction: Dermatophytic infections are commonly encountered a problem and constitute more than 50% of cases in dermatology outpatient departments. Diagnosis of these infections requires the proper use of laboratory methods. Objectives: This study was conducted to know the etiology of...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2015-07-01
|
| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.163135 |
| _version_ | 1818991680019234816 |
|---|---|
| author | Lakshmi Vasantha Poluri Jyothi P Indugula Sai L Kondapaneni |
| author_facet | Lakshmi Vasantha Poluri Jyothi P Indugula Sai L Kondapaneni |
| author_sort | Lakshmi Vasantha Poluri |
| collection | DOAJ |
| description | Introduction: Dermatophytic infections are commonly encountered a problem and constitute more than 50% of cases in dermatology outpatient departments. Diagnosis of these infections requires the proper use of laboratory methods.
Objectives: This study was conducted to know the etiology of dermatophytosis in patients attending Tertiary Care Level Hospital in South India and to compare the efficacy of Sabouraud’s dextrose agar (SDA) with actidione and dermatophyte test medium (DTM) in isolating and identifying dermatophytes.
Materials and Methods: A total of 110 samples which included 101 skin samples and 9 hair samples from clinically suspected dermatophytosis were collected. Direct microscopy by KOH and culture on SDA with actidione and DTM were done.
Results: Of 110 samples collected, 58.18% were KOH positive for fungal filaments and 56.36% were culture positive for dermatophytes. More number of cases were observed between age groups of 21–40 years. Males were more affected compared to females. Tinea corporis was the common clinical presentation observed (40%). Trichophyton rubrum (58.06%) was the predominant isolate recovered in all clinical presentations but Trichophyton violaceum was the most common isolate in tinea capitis. All culture positives were grown on both SDA with actidione and DTM. Appearance of growth was earlier on DTM that is, within 10 days compared to SDA with actidione where growth started appearing only after 10 days. This is statistically significant P < 0.0001 (X
2 = 71.6). Species level identification on primary isolation was possible when grown on SDA with actidione and it was not possible with the growth on DTM on primary isolation.
Conclusion: DTM is a good screening medium in laboratory diagnosis of dermatophytosis when compared to SDA with actidione. But DTM is inferior to SDA with actidione in identification of dermatophyte species. |
| first_indexed | 2024-12-20T20:14:06Z |
| format | Article |
| id | doaj.art-3d0ebf9135714056ae0124456e42b2af |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-20T20:14:06Z |
| publishDate | 2015-07-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-3d0ebf9135714056ae0124456e42b2af2022-12-21T19:27:46ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262015-07-0170208408910.4103/0974-2727.163135Clinicomycological Study of Dermatophytosis in South IndiaLakshmi Vasantha Poluri0Jyothi P Indugula1Sai L Kondapaneni2Department of Microbiology, Kamineni Institute of Medical Sciences, Nalgonda, TelanganaDepartment of Microbiology, Andhra Medical College, Visakhapatnam, Andhra Pradesh, IndiaDepartment of Microbiology, Kamineni Institute of Medical Sciences, Nalgonda, TelanganaIntroduction: Dermatophytic infections are commonly encountered a problem and constitute more than 50% of cases in dermatology outpatient departments. Diagnosis of these infections requires the proper use of laboratory methods. Objectives: This study was conducted to know the etiology of dermatophytosis in patients attending Tertiary Care Level Hospital in South India and to compare the efficacy of Sabouraud’s dextrose agar (SDA) with actidione and dermatophyte test medium (DTM) in isolating and identifying dermatophytes. Materials and Methods: A total of 110 samples which included 101 skin samples and 9 hair samples from clinically suspected dermatophytosis were collected. Direct microscopy by KOH and culture on SDA with actidione and DTM were done. Results: Of 110 samples collected, 58.18% were KOH positive for fungal filaments and 56.36% were culture positive for dermatophytes. More number of cases were observed between age groups of 21–40 years. Males were more affected compared to females. Tinea corporis was the common clinical presentation observed (40%). Trichophyton rubrum (58.06%) was the predominant isolate recovered in all clinical presentations but Trichophyton violaceum was the most common isolate in tinea capitis. All culture positives were grown on both SDA with actidione and DTM. Appearance of growth was earlier on DTM that is, within 10 days compared to SDA with actidione where growth started appearing only after 10 days. This is statistically significant P < 0.0001 (X 2 = 71.6). Species level identification on primary isolation was possible when grown on SDA with actidione and it was not possible with the growth on DTM on primary isolation. Conclusion: DTM is a good screening medium in laboratory diagnosis of dermatophytosis when compared to SDA with actidione. But DTM is inferior to SDA with actidione in identification of dermatophyte species.http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.163135dermatophte test mediumdermatophytosissabouraud’s dextrose agar with actidione |
| spellingShingle | Lakshmi Vasantha Poluri Jyothi P Indugula Sai L Kondapaneni Clinicomycological Study of Dermatophytosis in South India Journal of Laboratory Physicians dermatophte test medium dermatophytosis sabouraud’s dextrose agar with actidione |
| title | Clinicomycological Study of Dermatophytosis in South India |
| title_full | Clinicomycological Study of Dermatophytosis in South India |
| title_fullStr | Clinicomycological Study of Dermatophytosis in South India |
| title_full_unstemmed | Clinicomycological Study of Dermatophytosis in South India |
| title_short | Clinicomycological Study of Dermatophytosis in South India |
| title_sort | clinicomycological study of dermatophytosis in south india |
| topic | dermatophte test medium dermatophytosis sabouraud’s dextrose agar with actidione |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.163135 |
| work_keys_str_mv | AT lakshmivasanthapoluri clinicomycologicalstudyofdermatophytosisinsouthindia AT jyothipindugula clinicomycologicalstudyofdermatophytosisinsouthindia AT sailkondapaneni clinicomycologicalstudyofdermatophytosisinsouthindia |