Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery
Chronic wound sites have elevated levels of proteolytic enzymes that negate the activity of topically applied growth factors. bFGF encapsulated in gelatin/alginate coacervates was protected from protease and showed better activity than bFGF in solution; however, its activity decreased with particle...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/12/2548 |
| _version_ | 1797455765793931264 |
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| author | JongOk Lee Eunmi Ban Heejung Park Aeri Kim |
| author_facet | JongOk Lee Eunmi Ban Heejung Park Aeri Kim |
| author_sort | JongOk Lee |
| collection | DOAJ |
| description | Chronic wound sites have elevated levels of proteolytic enzymes that negate the activity of topically applied growth factors. bFGF encapsulated in gelatin/alginate coacervates was protected from protease and showed better activity than bFGF in solution; however, its activity decreased with particle size and PDI increase after freeze-drying and rehydration. In this study, we aim to improve the stability of bFGF coacervates during freeze-drying to enable a topically applied growth factor delivery system for diabetic foot ulcer. Trehalose, mannitol, and Tween 80 at various concentrations were tested as cryoprotectant candidates. Trehalose improved the mechanical property of freeze-dried coacervates and physical properties after rehydration, resulting in stable size and PDI values. It also enhanced the bFGF activity in hyperglycemic human dermal fibroblasts with better cell viability, migration, and procollagen synthesis compared to the coacervates without trehalose. Hydrogen bonding interactions between trehalose and polymers probed by ATR-FTIR contribute to the stability of coacervates during freeze-drying. In conclusion, the freeze-dried gelatin/alginate coacervates encapsulating bFGF was effectively stabilized with trehalose, and the resulting coacervate composition is suggested as a potential therapeutic modality for chronic wounds including diabetic foot ulcer. |
| first_indexed | 2024-03-09T15:58:13Z |
| format | Article |
| id | doaj.art-3d0ec21bb4014ae2a7528279591f0ac9 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T15:58:13Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-3d0ec21bb4014ae2a7528279591f0ac92023-11-24T17:17:50ZengMDPI AGPharmaceutics1999-49232022-11-011412254810.3390/pharmaceutics14122548Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF DeliveryJongOk Lee0Eunmi Ban1Heejung Park2Aeri Kim3Department of Pharmacy, College of Pharmacy, CHA University, Seongnam-si 463-400, Republic of KoreaDepartment of Pharmacy, College of Pharmacy, CHA University, Seongnam-si 463-400, Republic of KoreaDepartment of Pharmacy, College of Pharmacy, CHA University, Seongnam-si 463-400, Republic of KoreaDepartment of Pharmacy, College of Pharmacy, CHA University, Seongnam-si 463-400, Republic of KoreaChronic wound sites have elevated levels of proteolytic enzymes that negate the activity of topically applied growth factors. bFGF encapsulated in gelatin/alginate coacervates was protected from protease and showed better activity than bFGF in solution; however, its activity decreased with particle size and PDI increase after freeze-drying and rehydration. In this study, we aim to improve the stability of bFGF coacervates during freeze-drying to enable a topically applied growth factor delivery system for diabetic foot ulcer. Trehalose, mannitol, and Tween 80 at various concentrations were tested as cryoprotectant candidates. Trehalose improved the mechanical property of freeze-dried coacervates and physical properties after rehydration, resulting in stable size and PDI values. It also enhanced the bFGF activity in hyperglycemic human dermal fibroblasts with better cell viability, migration, and procollagen synthesis compared to the coacervates without trehalose. Hydrogen bonding interactions between trehalose and polymers probed by ATR-FTIR contribute to the stability of coacervates during freeze-drying. In conclusion, the freeze-dried gelatin/alginate coacervates encapsulating bFGF was effectively stabilized with trehalose, and the resulting coacervate composition is suggested as a potential therapeutic modality for chronic wounds including diabetic foot ulcer.https://www.mdpi.com/1999-4923/14/12/2548diabetic foot ulcercoacervatesfibroblast growth factorfreeze-dryingcryoprotectants |
| spellingShingle | JongOk Lee Eunmi Ban Heejung Park Aeri Kim Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery Pharmaceutics diabetic foot ulcer coacervates fibroblast growth factor freeze-drying cryoprotectants |
| title | Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery |
| title_full | Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery |
| title_fullStr | Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery |
| title_full_unstemmed | Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery |
| title_short | Stability Enhancement of Freeze-Dried Gelatin/Alginate Coacervates for bFGF Delivery |
| title_sort | stability enhancement of freeze dried gelatin alginate coacervates for bfgf delivery |
| topic | diabetic foot ulcer coacervates fibroblast growth factor freeze-drying cryoprotectants |
| url | https://www.mdpi.com/1999-4923/14/12/2548 |
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