Multiple Roles and Effects of a Novel Trichoderma Hydrophobin
Fungi belonging to the genus Trichoderma are among the most active and ecologically successful microbes found in natural environments, because they are able to use a variety of substrates and affect the growth of other microbes and virtually any plant species. We isolated and characterized a novel t...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2015-02-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-14-0194-R |
| _version_ | 1811287286842654720 |
|---|---|
| author | Michelina Ruocco Stefania Lanzuise Nadia Lombardi Sheridan L. Woo Francesco Vinale Roberta Marra Rosaria Varlese Gelsomina Manganiello Alberto Pascale Valeria Scala David Turrà Felice Scala Matteo Lorito |
| author_facet | Michelina Ruocco Stefania Lanzuise Nadia Lombardi Sheridan L. Woo Francesco Vinale Roberta Marra Rosaria Varlese Gelsomina Manganiello Alberto Pascale Valeria Scala David Turrà Felice Scala Matteo Lorito |
| author_sort | Michelina Ruocco |
| collection | DOAJ |
| description | Fungi belonging to the genus Trichoderma are among the most active and ecologically successful microbes found in natural environments, because they are able to use a variety of substrates and affect the growth of other microbes and virtually any plant species. We isolated and characterized a novel type II hydrophobin secreted by the biocontrol strain MK1 of Trichoderma longibrachiatum. The corresponding gene (Hytlo1) has a multiple role in the Trichoderma–plant–pathogen three-way interaction, while the purified protein displayed a direct antifungal as well as a microbe-associated molecular pattern and a plant growth promotion (PGP) activity. Leaf infiltration with the hydrophobin systemically increased resistance to pathogens and activated defense-related responses involving reactive oxygen species, superoxide dismutase, oxylipin, phytoalexin, and pathogenesis-related protein formation or activity. The hydrophobin was found to enhance development of a variety of plants when applied at very low doses. It particularly stimulated root formation and growth, as demonstrated also by transient expression of the encoding gene in tobacco and tomato. Targeted knock-out of Hytlo1 significantly reduced both antagonistic and PGP effect of the wild-type strain. We conclude that this protein represents a clear example of a molecular factor developed by Trichoderma spp. to establish a mutually beneficial interaction with the colonized plant. |
| first_indexed | 2024-04-13T03:16:16Z |
| format | Article |
| id | doaj.art-3d140654ca5540eebb5e2bf9f43a33d5 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:16:16Z |
| publishDate | 2015-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-3d140654ca5540eebb5e2bf9f43a33d52022-12-22T03:04:54ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062015-02-0128216717910.1094/MPMI-07-14-0194-RMultiple Roles and Effects of a Novel Trichoderma HydrophobinMichelina RuoccoStefania LanzuiseNadia LombardiSheridan L. WooFrancesco VinaleRoberta MarraRosaria VarleseGelsomina ManganielloAlberto PascaleValeria ScalaDavid TurràFelice ScalaMatteo LoritoFungi belonging to the genus Trichoderma are among the most active and ecologically successful microbes found in natural environments, because they are able to use a variety of substrates and affect the growth of other microbes and virtually any plant species. We isolated and characterized a novel type II hydrophobin secreted by the biocontrol strain MK1 of Trichoderma longibrachiatum. The corresponding gene (Hytlo1) has a multiple role in the Trichoderma–plant–pathogen three-way interaction, while the purified protein displayed a direct antifungal as well as a microbe-associated molecular pattern and a plant growth promotion (PGP) activity. Leaf infiltration with the hydrophobin systemically increased resistance to pathogens and activated defense-related responses involving reactive oxygen species, superoxide dismutase, oxylipin, phytoalexin, and pathogenesis-related protein formation or activity. The hydrophobin was found to enhance development of a variety of plants when applied at very low doses. It particularly stimulated root formation and growth, as demonstrated also by transient expression of the encoding gene in tobacco and tomato. Targeted knock-out of Hytlo1 significantly reduced both antagonistic and PGP effect of the wild-type strain. We conclude that this protein represents a clear example of a molecular factor developed by Trichoderma spp. to establish a mutually beneficial interaction with the colonized plant.https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-14-0194-R |
| spellingShingle | Michelina Ruocco Stefania Lanzuise Nadia Lombardi Sheridan L. Woo Francesco Vinale Roberta Marra Rosaria Varlese Gelsomina Manganiello Alberto Pascale Valeria Scala David Turrà Felice Scala Matteo Lorito Multiple Roles and Effects of a Novel Trichoderma Hydrophobin Molecular Plant-Microbe Interactions |
| title | Multiple Roles and Effects of a Novel Trichoderma Hydrophobin |
| title_full | Multiple Roles and Effects of a Novel Trichoderma Hydrophobin |
| title_fullStr | Multiple Roles and Effects of a Novel Trichoderma Hydrophobin |
| title_full_unstemmed | Multiple Roles and Effects of a Novel Trichoderma Hydrophobin |
| title_short | Multiple Roles and Effects of a Novel Trichoderma Hydrophobin |
| title_sort | multiple roles and effects of a novel trichoderma hydrophobin |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-14-0194-R |
| work_keys_str_mv | AT michelinaruocco multiplerolesandeffectsofanoveltrichodermahydrophobin AT stefanialanzuise multiplerolesandeffectsofanoveltrichodermahydrophobin AT nadialombardi multiplerolesandeffectsofanoveltrichodermahydrophobin AT sheridanlwoo multiplerolesandeffectsofanoveltrichodermahydrophobin AT francescovinale multiplerolesandeffectsofanoveltrichodermahydrophobin AT robertamarra multiplerolesandeffectsofanoveltrichodermahydrophobin AT rosariavarlese multiplerolesandeffectsofanoveltrichodermahydrophobin AT gelsominamanganiello multiplerolesandeffectsofanoveltrichodermahydrophobin AT albertopascale multiplerolesandeffectsofanoveltrichodermahydrophobin AT valeriascala multiplerolesandeffectsofanoveltrichodermahydrophobin AT davidturra multiplerolesandeffectsofanoveltrichodermahydrophobin AT felicescala multiplerolesandeffectsofanoveltrichodermahydrophobin AT matteolorito multiplerolesandeffectsofanoveltrichodermahydrophobin |