Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes
Abstract Background Staphylococcus aureus, one of the most prevalent opportunistic pathogens, mainly colonizes the nasal cavity and is a risk factor for severe infections. Virulence factors and accessory gene regulator (agr) are key to the severity and diversity of staphylococcal infection. In this...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-03126-y |
| _version_ | 1797415824628121600 |
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| author | Hao Cai Xueying Li Chao Zhang Huamin Zhong Yongqiang Xie Lianfen Huang Baidu Zhang Yan Long Zhenwen Zhou Bingshao Liang |
| author_facet | Hao Cai Xueying Li Chao Zhang Huamin Zhong Yongqiang Xie Lianfen Huang Baidu Zhang Yan Long Zhenwen Zhou Bingshao Liang |
| author_sort | Hao Cai |
| collection | DOAJ |
| description | Abstract Background Staphylococcus aureus, one of the most prevalent opportunistic pathogens, mainly colonizes the nasal cavity and is a risk factor for severe infections. Virulence factors and accessory gene regulator (agr) are key to the severity and diversity of staphylococcal infection. In this study, we aimed to characterise S. aureus agr-types and virulence genes and correlated them with genetic background and antibiotic-resistant phenotypes. Results Agr types were identified in 704 isolates (98.5%), with only 11 isolates were negative for agr type. Most of our isolates were classified as agr type I, followed by types III, II and IV. The enterotoxin c gene (sec) was detected in 48.6% of isolates, showing the highest prevalence among the five enterotoxin genes detected. The positivity rates for the lukS/F-PV and tsst genes were 4% and 2.2%, respectively, while neither sed nor SasX were detected. ST45, ST59, ST338, ST188, ST6, ST7, ST22, ST25, ST398, and ST944 belonged to agr I group, while ST5 and ST15 belonged to agr II group. ST30 and ST1 were classified into agr III group, and ST121 was assigned into agr IV group. The tsst gene was found exclusively within agr I and III types belonging to ST7 and ST30 isolates, while the lukS/F-PV was predominantly carried by agr I type isolates primarily within CC59 and CC22 clones. Among the methicillin-resistant S. aureus (MRSA) isolates, 89.7% belonged to agr I group, and 97.8% of rifampicin-resistant or intermediate isolates were assigned to agr I group. MRSA isolates harboured more tested virulence genes compared to methicillin-susceptible S. aureus isolates. Conclusions We characterized the distributions of agr types and eight major virulence genes of 715 S. aureus isolates, and our findings revealed clear associations between agr types and STs, as well as virulence genes, and drug resistant phenotypes. |
| first_indexed | 2024-03-09T05:55:09Z |
| format | Article |
| id | doaj.art-3d14c65e890142b69d293538ecc736dc |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T05:55:09Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-3d14c65e890142b69d293538ecc736dc2023-12-03T12:14:05ZengBMCBMC Microbiology1471-21802023-11-012311710.1186/s12866-023-03126-yMolecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypesHao Cai0Xueying Li1Chao Zhang2Huamin Zhong3Yongqiang Xie4Lianfen Huang5Baidu Zhang6Yan Long7Zhenwen Zhou8Bingshao Liang9Clinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityNational Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor InstituteClinical Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical UniversityAbstract Background Staphylococcus aureus, one of the most prevalent opportunistic pathogens, mainly colonizes the nasal cavity and is a risk factor for severe infections. Virulence factors and accessory gene regulator (agr) are key to the severity and diversity of staphylococcal infection. In this study, we aimed to characterise S. aureus agr-types and virulence genes and correlated them with genetic background and antibiotic-resistant phenotypes. Results Agr types were identified in 704 isolates (98.5%), with only 11 isolates were negative for agr type. Most of our isolates were classified as agr type I, followed by types III, II and IV. The enterotoxin c gene (sec) was detected in 48.6% of isolates, showing the highest prevalence among the five enterotoxin genes detected. The positivity rates for the lukS/F-PV and tsst genes were 4% and 2.2%, respectively, while neither sed nor SasX were detected. ST45, ST59, ST338, ST188, ST6, ST7, ST22, ST25, ST398, and ST944 belonged to agr I group, while ST5 and ST15 belonged to agr II group. ST30 and ST1 were classified into agr III group, and ST121 was assigned into agr IV group. The tsst gene was found exclusively within agr I and III types belonging to ST7 and ST30 isolates, while the lukS/F-PV was predominantly carried by agr I type isolates primarily within CC59 and CC22 clones. Among the methicillin-resistant S. aureus (MRSA) isolates, 89.7% belonged to agr I group, and 97.8% of rifampicin-resistant or intermediate isolates were assigned to agr I group. MRSA isolates harboured more tested virulence genes compared to methicillin-susceptible S. aureus isolates. Conclusions We characterized the distributions of agr types and eight major virulence genes of 715 S. aureus isolates, and our findings revealed clear associations between agr types and STs, as well as virulence genes, and drug resistant phenotypes.https://doi.org/10.1186/s12866-023-03126-yAgr typingVirulence factorsMulti-locus sequence typingAntibiotic resistanceStaphylococcus aureus |
| spellingShingle | Hao Cai Xueying Li Chao Zhang Huamin Zhong Yongqiang Xie Lianfen Huang Baidu Zhang Yan Long Zhenwen Zhou Bingshao Liang Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes BMC Microbiology Agr typing Virulence factors Multi-locus sequence typing Antibiotic resistance Staphylococcus aureus |
| title | Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes |
| title_full | Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes |
| title_fullStr | Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes |
| title_full_unstemmed | Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes |
| title_short | Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes |
| title_sort | molecular characterisation of staphylococcus aureus in school age children in guangzhou associations among agr types virulence genes sequence types and antibiotic resistant phenotypes |
| topic | Agr typing Virulence factors Multi-locus sequence typing Antibiotic resistance Staphylococcus aureus |
| url | https://doi.org/10.1186/s12866-023-03126-y |
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