Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia
Background and Objective: Identification of cytogenetic and molecular changes plays an important role in acute myeloid leukemia (AML) patients. Thus, they are used in classification, prognosis and treatment of the disease. The CD123 expression and FLT3 gene mutations are also the variations that may...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pathology
2018-10-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_32424_ce2ae7f38cdf4ce4158722ef3db51787.pdf |
| _version_ | 1831716847499608064 |
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| author | Freidoon Solhjoo Akbar Safaie Ahmad Monabati Maral Mokhtari Moeinadin Safavi |
| author_facet | Freidoon Solhjoo Akbar Safaie Ahmad Monabati Maral Mokhtari Moeinadin Safavi |
| author_sort | Freidoon Solhjoo |
| collection | DOAJ |
| description | Background and Objective: Identification of cytogenetic and molecular changes plays an important role in acute myeloid leukemia (AML) patients. Thus, they are used in classification, prognosis and treatment of the disease. The CD123 expression and FLT3 gene mutations are also the variations that may assist in prognosis and treatment of patients with AML.Methods: This study was performed on 76 patients as new cases of AML. The correlation between CD123 immunohistochemical (IHC) expression and FLT3 gene mutations with each other as well as morphological, immunophenotypical and cytogenetic factors was studied.Results: The results represented the CD123 IHC expression in 55.3% and FLT3 gene mutations in 28.9% of cases. We found that 81.3% of patients who had FLT3/ITD gene mutations revealed IHC of CD123 expression (P=0.019). The CD123 expression against FLT3 was also correlated with monocytic differentiation in bone marrow blasts (P=0.031). There were significant correlations between IHC expression of CD123 and FLT3/ITD mutations with a high percentage of aspirated bone marrow blasts (P=0.01 and P=0.006, respectively) as well as the lack of CD34 expression in bone marrow blasts (P=0.007 and P=0.021, respectively).Conclusion: The CD123 IHC positive AMLs were correlated with certain pathologic features, some of which can be similar with correlations of background mutation of FLT3/ITD; According to the negative predictive value (NPV), 88.2% of CD123 IHC showed FLT3 gene mutation. In addition to its use in targeted therapy, it could be a marker to decide what molecular tests to use in the next steps. |
| first_indexed | 2024-12-21T00:47:22Z |
| format | Article |
| id | doaj.art-3d16cccf78244d6b975f81dff48c8806 |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-21T00:47:22Z |
| publishDate | 2018-10-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-3d16cccf78244d6b975f81dff48c88062022-12-21T19:21:29ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562018-10-0113443844632424Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid LeukemiaFreidoon Solhjoo0Akbar Safaie1Ahmad Monabati2Maral Mokhtari3Moeinadin Safavi4Dept. of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDept. of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDept. of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDept. of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDept. of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBackground and Objective: Identification of cytogenetic and molecular changes plays an important role in acute myeloid leukemia (AML) patients. Thus, they are used in classification, prognosis and treatment of the disease. The CD123 expression and FLT3 gene mutations are also the variations that may assist in prognosis and treatment of patients with AML.Methods: This study was performed on 76 patients as new cases of AML. The correlation between CD123 immunohistochemical (IHC) expression and FLT3 gene mutations with each other as well as morphological, immunophenotypical and cytogenetic factors was studied.Results: The results represented the CD123 IHC expression in 55.3% and FLT3 gene mutations in 28.9% of cases. We found that 81.3% of patients who had FLT3/ITD gene mutations revealed IHC of CD123 expression (P=0.019). The CD123 expression against FLT3 was also correlated with monocytic differentiation in bone marrow blasts (P=0.031). There were significant correlations between IHC expression of CD123 and FLT3/ITD mutations with a high percentage of aspirated bone marrow blasts (P=0.01 and P=0.006, respectively) as well as the lack of CD34 expression in bone marrow blasts (P=0.007 and P=0.021, respectively).Conclusion: The CD123 IHC positive AMLs were correlated with certain pathologic features, some of which can be similar with correlations of background mutation of FLT3/ITD; According to the negative predictive value (NPV), 88.2% of CD123 IHC showed FLT3 gene mutation. In addition to its use in targeted therapy, it could be a marker to decide what molecular tests to use in the next steps.https://ijp.iranpath.org/article_32424_ce2ae7f38cdf4ce4158722ef3db51787.pdfacute myeloid leukemiafms-like tyrosine kinase 3cd123karyotype |
| spellingShingle | Freidoon Solhjoo Akbar Safaie Ahmad Monabati Maral Mokhtari Moeinadin Safavi Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia Iranian Journal of Pathology acute myeloid leukemia fms-like tyrosine kinase 3 cd123 karyotype |
| title | Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia |
| title_full | Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia |
| title_fullStr | Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia |
| title_full_unstemmed | Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia |
| title_short | Evaluation of the CD123 Expression and FLT3 Gene Mutations in Patients with Acute Myeloid Leukemia |
| title_sort | evaluation of the cd123 expression and flt3 gene mutations in patients with acute myeloid leukemia |
| topic | acute myeloid leukemia fms-like tyrosine kinase 3 cd123 karyotype |
| url | https://ijp.iranpath.org/article_32424_ce2ae7f38cdf4ce4158722ef3db51787.pdf |
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