Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora
Sponge diseases have been widely reported, yet the causal factors and major pathogenic microbes remain elusive. In this study, two individuals of the sponge Crella cyathophora in total that showed similar disease-like characteristics were collected from two different locations along the Red Sea coas...
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PeerJ Inc.
2015-06-01
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| Online Access: | https://peerj.com/articles/890.pdf |
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| author | Zhao-Ming Gao Yong Wang Ren-Mao Tian On On Lee Yue Him Wong Zenon B. Batang Abdulaziz Al-Suwailem Feras F. Lafi Vladimir B. Bajic Pei-Yuan Qian |
| author_facet | Zhao-Ming Gao Yong Wang Ren-Mao Tian On On Lee Yue Him Wong Zenon B. Batang Abdulaziz Al-Suwailem Feras F. Lafi Vladimir B. Bajic Pei-Yuan Qian |
| author_sort | Zhao-Ming Gao |
| collection | DOAJ |
| description | Sponge diseases have been widely reported, yet the causal factors and major pathogenic microbes remain elusive. In this study, two individuals of the sponge Crella cyathophora in total that showed similar disease-like characteristics were collected from two different locations along the Red Sea coast separated by more than 30 kilometers. The disease-like parts of the two individuals were both covered by green surfaces, and the body size was much smaller compared with adjacent healthy regions. Here, using high-throughput pyrosequencing technology, we investigated the prokaryotic communities in healthy and disease-like sponge tissues as well as adjacent seawater. Microbes in healthy tissues belonged mainly to the Proteobacteria, Cyanobacteria and Bacteroidetes, and were much more diverse at the phylum level than reported previously. Interestingly, the disease-like tissues from the two sponge individuals underwent shifts of prokaryotic communities and were both enriched with a novel clade affiliated with the phylum Verrucomicrobia, implying its intimate connection with the disease-like Red Sea sponge C. cyathophora. Enrichment of the phylum Verrucomicrobia was also considered to be correlated with the presence of algae assemblages forming the green surface of the disease-like sponge tissues. This finding represents an interesting case of sponge disease and is valuable for further study. |
| first_indexed | 2024-03-09T06:29:49Z |
| format | Article |
| id | doaj.art-3d194c14f89342c79bdeb1749c3a3dc8 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:29:49Z |
| publishDate | 2015-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-3d194c14f89342c79bdeb1749c3a3dc82023-12-03T11:06:49ZengPeerJ Inc.PeerJ2167-83592015-06-013e89010.7717/peerj.890890Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophoraZhao-Ming Gao0Yong Wang1Ren-Mao Tian2On On Lee3Yue Him Wong4Zenon B. Batang5Abdulaziz Al-Suwailem6Feras F. Lafi7Vladimir B. Bajic8Pei-Yuan Qian9Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR ChinaDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR ChinaDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR ChinaDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR ChinaDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR ChinaCoastal and Marine Resources Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaCoastal and Marine Resources Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaComputational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaComputational Bioscience Research Center (CBRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDivision of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, PR ChinaSponge diseases have been widely reported, yet the causal factors and major pathogenic microbes remain elusive. In this study, two individuals of the sponge Crella cyathophora in total that showed similar disease-like characteristics were collected from two different locations along the Red Sea coast separated by more than 30 kilometers. The disease-like parts of the two individuals were both covered by green surfaces, and the body size was much smaller compared with adjacent healthy regions. Here, using high-throughput pyrosequencing technology, we investigated the prokaryotic communities in healthy and disease-like sponge tissues as well as adjacent seawater. Microbes in healthy tissues belonged mainly to the Proteobacteria, Cyanobacteria and Bacteroidetes, and were much more diverse at the phylum level than reported previously. Interestingly, the disease-like tissues from the two sponge individuals underwent shifts of prokaryotic communities and were both enriched with a novel clade affiliated with the phylum Verrucomicrobia, implying its intimate connection with the disease-like Red Sea sponge C. cyathophora. Enrichment of the phylum Verrucomicrobia was also considered to be correlated with the presence of algae assemblages forming the green surface of the disease-like sponge tissues. This finding represents an interesting case of sponge disease and is valuable for further study.https://peerj.com/articles/890.pdfLow microbial abundanceVerrucomicrobiaSponge symbiontDisease-like sponge |
| spellingShingle | Zhao-Ming Gao Yong Wang Ren-Mao Tian On On Lee Yue Him Wong Zenon B. Batang Abdulaziz Al-Suwailem Feras F. Lafi Vladimir B. Bajic Pei-Yuan Qian Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora PeerJ Low microbial abundance Verrucomicrobia Sponge symbiont Disease-like sponge |
| title | Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora |
| title_full | Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora |
| title_fullStr | Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora |
| title_full_unstemmed | Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora |
| title_short | Pyrosequencing revealed shifts of prokaryotic communities between healthy and disease-like tissues of the Red Sea sponge Crella cyathophora |
| title_sort | pyrosequencing revealed shifts of prokaryotic communities between healthy and disease like tissues of the red sea sponge crella cyathophora |
| topic | Low microbial abundance Verrucomicrobia Sponge symbiont Disease-like sponge |
| url | https://peerj.com/articles/890.pdf |
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