Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF
Lymphedema is a chronic and progressive condition that causes physical disfigurement and psychological trauma due to the accumulation of lymphatic fluid in the interstitial space. Once it develops, lymphedema is difficult to treat because it leads to the fibrosis of adipose tissue. However, the mech...
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MDPI AG
2023-09-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/10/507 |
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| author | Takashi Nuri Denan Jin Shinji Takai Koichi Ueda |
| author_facet | Takashi Nuri Denan Jin Shinji Takai Koichi Ueda |
| author_sort | Takashi Nuri |
| collection | DOAJ |
| description | Lymphedema is a chronic and progressive condition that causes physical disfigurement and psychological trauma due to the accumulation of lymphatic fluid in the interstitial space. Once it develops, lymphedema is difficult to treat because it leads to the fibrosis of adipose tissue. However, the mechanism behind this remains unclear. The purpose of this study was to investigate the involvement of mast cells (MCs) in the adipose tissues of patients with lymphedema. We found that fibrosis spread through blood vessels in the adipose tissues of lymphedema patients, and the expression of the collagen I and III genes was significantly increased compared to that of those in normal adipose tissue. Immunostaining of vimentin and α-smooth muscle actin showed that fibroblasts were the main cellular components in severely fibrotic regions. Toluidine blue staining confirmed a significant increase in the number of MCs in the adipose tissues of lymphedema patients, and immunostaining of serial sections of adipose tissue showed a significant increase in the number of tryptase-positive cells in lymphedema tissues compared with those in normal adipose tissues. Linear regression analyses revealed significant positive correlations between tryptase and the expressions of the TNF-α, platelet-derived growth factor (PDGF)-A, and PDGFR-α genes. PDGF-A–positive staining was observed in both fibroblasts and granules of tryptase-positive MCs. These results suggest that MC-derived tryptase plays a role in the fibrosis of adipose tissue due to lymphedema directly or in cooperation with other mediators. |
| first_indexed | 2024-03-10T21:20:28Z |
| format | Article |
| id | doaj.art-3d19f3d492e24a18830480142b190625 |
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| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T21:20:28Z |
| publishDate | 2023-09-01 |
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| spelling | doaj.art-3d19f3d492e24a18830480142b1906252023-11-19T16:06:28ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-09-0145108027803910.3390/cimb45100507Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGFTakashi Nuri0Denan Jin1Shinji Takai2Koichi Ueda3Department of Plastic and Reconstructive Surgery, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, JapanDepartment of Innovative Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, JapanDepartment of Innovative Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, JapanDepartment of Plastic and Reconstructive Surgery, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, JapanLymphedema is a chronic and progressive condition that causes physical disfigurement and psychological trauma due to the accumulation of lymphatic fluid in the interstitial space. Once it develops, lymphedema is difficult to treat because it leads to the fibrosis of adipose tissue. However, the mechanism behind this remains unclear. The purpose of this study was to investigate the involvement of mast cells (MCs) in the adipose tissues of patients with lymphedema. We found that fibrosis spread through blood vessels in the adipose tissues of lymphedema patients, and the expression of the collagen I and III genes was significantly increased compared to that of those in normal adipose tissue. Immunostaining of vimentin and α-smooth muscle actin showed that fibroblasts were the main cellular components in severely fibrotic regions. Toluidine blue staining confirmed a significant increase in the number of MCs in the adipose tissues of lymphedema patients, and immunostaining of serial sections of adipose tissue showed a significant increase in the number of tryptase-positive cells in lymphedema tissues compared with those in normal adipose tissues. Linear regression analyses revealed significant positive correlations between tryptase and the expressions of the TNF-α, platelet-derived growth factor (PDGF)-A, and PDGFR-α genes. PDGF-A–positive staining was observed in both fibroblasts and granules of tryptase-positive MCs. These results suggest that MC-derived tryptase plays a role in the fibrosis of adipose tissue due to lymphedema directly or in cooperation with other mediators.https://www.mdpi.com/1467-3045/45/10/507tryptasemast cellssecondary lymphedemaadipose fibrosisplatelet-derived growth factor A (PDGF-A) |
| spellingShingle | Takashi Nuri Denan Jin Shinji Takai Koichi Ueda Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF Current Issues in Molecular Biology tryptase mast cells secondary lymphedema adipose fibrosis platelet-derived growth factor A (PDGF-A) |
| title | Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF |
| title_full | Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF |
| title_fullStr | Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF |
| title_full_unstemmed | Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF |
| title_short | Tryptase-Positive Mast Cells Promote Adipose Fibrosis in Secondary Lymphedema through PDGF |
| title_sort | tryptase positive mast cells promote adipose fibrosis in secondary lymphedema through pdgf |
| topic | tryptase mast cells secondary lymphedema adipose fibrosis platelet-derived growth factor A (PDGF-A) |
| url | https://www.mdpi.com/1467-3045/45/10/507 |
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