Enrichment strategies for phosphoproteomics: state-of-the-art

Protein phosphorylation is a key regulator in many biological processes, such as homeostasis, cellular signaling and communication, transcriptional and translational regulation, and apoptosis. The defects in this tightly controlled reversible post-translational modification have been described to co...

Full description

Bibliographic Details
Main Authors: Salovska Barbora, Tichy Ales, Rezacova Martina, Vavrova Jirina, Novotna Eva
Format: Article
Language:English
Published: De Gruyter 2012-03-01
Series:Reviews in Analytical Chemistry
Subjects:
Online Access:https://doi.org/10.1515/revac-2011-0025
_version_ 1819147017807462400
author Salovska Barbora
Tichy Ales
Rezacova Martina
Vavrova Jirina
Novotna Eva
author_facet Salovska Barbora
Tichy Ales
Rezacova Martina
Vavrova Jirina
Novotna Eva
author_sort Salovska Barbora
collection DOAJ
description Protein phosphorylation is a key regulator in many biological processes, such as homeostasis, cellular signaling and communication, transcriptional and translational regulation, and apoptosis. The defects in this tightly controlled reversible post-translational modification have been described to contribute to genesis and progression of various diseases, emphasizing the importance of a systematic research of this phenomenon. Although considerable effort has been devoted to improving the analysis of phosphorylation by mass spectrometry, which is currently the method of choice to study protein phosphorylation, the detection and identification of phosphorylation sites remains challenging because of the low abundance and low ionization efficacy of phosphoproteins in comparison with nonphosphorylated proteins. To overcome this obstacle, different enrichment strategies for phosphorylated peptides/proteins have been established and optimized for subsequent mass spectrometry analysis. In this review, we will give an overview of the methods currently available for the enrichment of phosphorylated proteins and peptides including immunoprecipitation, chemical derivatization and affinity enrichment techniques.
first_indexed 2024-12-22T13:23:08Z
format Article
id doaj.art-3d1b966c61de4fa5810ee4f9b3169a09
institution Directory Open Access Journal
issn 0793-0135
2191-0189
language English
last_indexed 2024-12-22T13:23:08Z
publishDate 2012-03-01
publisher De Gruyter
record_format Article
series Reviews in Analytical Chemistry
spelling doaj.art-3d1b966c61de4fa5810ee4f9b3169a092022-12-21T18:24:24ZengDe GruyterReviews in Analytical Chemistry0793-01352191-01892012-03-01311294110.1515/revac-2011-0025Enrichment strategies for phosphoproteomics: state-of-the-artSalovska BarboraTichy AlesRezacova Martina0Vavrova Jirina1Novotna Eva2Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Hradec Králové, Czech RepublicDepartment of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech RepublicDepartment of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech RepublicProtein phosphorylation is a key regulator in many biological processes, such as homeostasis, cellular signaling and communication, transcriptional and translational regulation, and apoptosis. The defects in this tightly controlled reversible post-translational modification have been described to contribute to genesis and progression of various diseases, emphasizing the importance of a systematic research of this phenomenon. Although considerable effort has been devoted to improving the analysis of phosphorylation by mass spectrometry, which is currently the method of choice to study protein phosphorylation, the detection and identification of phosphorylation sites remains challenging because of the low abundance and low ionization efficacy of phosphoproteins in comparison with nonphosphorylated proteins. To overcome this obstacle, different enrichment strategies for phosphorylated peptides/proteins have been established and optimized for subsequent mass spectrometry analysis. In this review, we will give an overview of the methods currently available for the enrichment of phosphorylated proteins and peptides including immunoprecipitation, chemical derivatization and affinity enrichment techniques.https://doi.org/10.1515/revac-2011-0025metal oxide affinity chromatographyphosphopeptide enrichmentphosphoproteomics
spellingShingle Salovska Barbora
Tichy Ales
Rezacova Martina
Vavrova Jirina
Novotna Eva
Enrichment strategies for phosphoproteomics: state-of-the-art
Reviews in Analytical Chemistry
metal oxide affinity chromatography
phosphopeptide enrichment
phosphoproteomics
title Enrichment strategies for phosphoproteomics: state-of-the-art
title_full Enrichment strategies for phosphoproteomics: state-of-the-art
title_fullStr Enrichment strategies for phosphoproteomics: state-of-the-art
title_full_unstemmed Enrichment strategies for phosphoproteomics: state-of-the-art
title_short Enrichment strategies for phosphoproteomics: state-of-the-art
title_sort enrichment strategies for phosphoproteomics state of the art
topic metal oxide affinity chromatography
phosphopeptide enrichment
phosphoproteomics
url https://doi.org/10.1515/revac-2011-0025
work_keys_str_mv AT salovskabarbora enrichmentstrategiesforphosphoproteomicsstateoftheart
AT tichyales enrichmentstrategiesforphosphoproteomicsstateoftheart
AT rezacovamartina enrichmentstrategiesforphosphoproteomicsstateoftheart
AT vavrovajirina enrichmentstrategiesforphosphoproteomicsstateoftheart
AT novotnaeva enrichmentstrategiesforphosphoproteomicsstateoftheart