Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes
Real-time quantitative polymerase chain reaction (RQ-PCR) for fusion transcripts and flow cytometry for leukemia-specific markers are widely used for minimal residual disease (MRD) detection in acute lymphoblastic leukemia, but the relation between the results of either method is unclear. Methods: M...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of the Formosan Medical Association |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664616304806 |
| _version_ | 1819018982335709184 |
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| author | Ying-Jung Huang Elaine Coustan-Smith Hsiao-Wen Kao Hsi-Che Liu Shih-Hsiang Chen Chih-Cheng Hsiao Chao-Ping Yang Tang-Her Jaing Ting-Chi Yeh Ming-Chung Kuo Chang-Liang Lai Chia-Hui Chang Dario Campana Der-Cherng Liang Lee-Yung Shih |
| author_facet | Ying-Jung Huang Elaine Coustan-Smith Hsiao-Wen Kao Hsi-Che Liu Shih-Hsiang Chen Chih-Cheng Hsiao Chao-Ping Yang Tang-Her Jaing Ting-Chi Yeh Ming-Chung Kuo Chang-Liang Lai Chia-Hui Chang Dario Campana Der-Cherng Liang Lee-Yung Shih |
| author_sort | Ying-Jung Huang |
| collection | DOAJ |
| description | Real-time quantitative polymerase chain reaction (RQ-PCR) for fusion transcripts and flow cytometry for leukemia-specific markers are widely used for minimal residual disease (MRD) detection in acute lymphoblastic leukemia, but the relation between the results of either method is unclear.
Methods: Mononucleated cells from 108 bone marrow samples collected from 55 B-precursor acute lymphoblastic leukemia patients (30 with t(12;21)/ETV6-RUNX1, 16 with t(9;22)/BCR-ABL1 and nine with t(1;19)/TCF3-PBX1) were examined in tandem by RQ-PCR and six-color flow cytometry.
Results: MRD results were concordant in 91 of the 108 paired samples (84.2%; K=0.690); 49 samples were MRD-negative while 42 were MRD-positive by both methods, with < 1 log difference in positive MRD estimates in 39 samples (92.9%). Of the 17 discordant samples, 16 were MRD-positive by RQ-PCR but MRD-negative by flow cytometry; the opposite was true in one sample. Kappa value/concordance was 0.690/85.0% (n = 60) for ETV6-RUNX1, 0.842/93.3% (n = 15) for TCF3-PBX1, and 0.535/78.8% (n = 33) for BCR-ABL1. Specific immunophenotypic abnormalities were more prevalent in each genetic subgroup, such as CD38 underexpression, CD58 overexpression, and CD34 overexpression in ETV6-RUNX1, TCF3-PBX1, and BCR-ABL1, respectively.
Conclusion: In most follow-up samples, MRD estimates by two methods are in agreement, especially in patients with TCF3-PBX1. |
| first_indexed | 2024-12-21T03:28:04Z |
| format | Article |
| id | doaj.art-3d1bcaee58e84ded9e8acaf630e74148 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-21T03:28:04Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-3d1bcaee58e84ded9e8acaf630e741482022-12-21T19:17:32ZengElsevierJournal of the Formosan Medical Association0929-66462017-10-011161077478110.1016/j.jfma.2016.12.002Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypesYing-Jung Huang0Elaine Coustan-Smith1Hsiao-Wen Kao2Hsi-Che Liu3Shih-Hsiang Chen4Chih-Cheng Hsiao5Chao-Ping Yang6Tang-Her Jaing7Ting-Chi Yeh8Ming-Chung Kuo9Chang-Liang Lai10Chia-Hui Chang11Dario Campana12Der-Cherng Liang13Lee-Yung Shih14Division of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Pediatrics, National University of Singapore, SingaporeDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Pediatrics, Mackay Memorial Hospital and Mackay Medical College, Taipei, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Hematology-Oncology, Chang Gung Children's Hospital at Linkou, Toayuan, TaiwanCollege of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Pediatrics, Mackay Memorial Hospital and Mackay Medical College, Taipei, TaiwanDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Pediatrics, National University of Singapore, SingaporeDepartment of Pediatrics, Mackay Memorial Hospital and Mackay Medical College, Taipei, TaiwanDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanReal-time quantitative polymerase chain reaction (RQ-PCR) for fusion transcripts and flow cytometry for leukemia-specific markers are widely used for minimal residual disease (MRD) detection in acute lymphoblastic leukemia, but the relation between the results of either method is unclear. Methods: Mononucleated cells from 108 bone marrow samples collected from 55 B-precursor acute lymphoblastic leukemia patients (30 with t(12;21)/ETV6-RUNX1, 16 with t(9;22)/BCR-ABL1 and nine with t(1;19)/TCF3-PBX1) were examined in tandem by RQ-PCR and six-color flow cytometry. Results: MRD results were concordant in 91 of the 108 paired samples (84.2%; K=0.690); 49 samples were MRD-negative while 42 were MRD-positive by both methods, with < 1 log difference in positive MRD estimates in 39 samples (92.9%). Of the 17 discordant samples, 16 were MRD-positive by RQ-PCR but MRD-negative by flow cytometry; the opposite was true in one sample. Kappa value/concordance was 0.690/85.0% (n = 60) for ETV6-RUNX1, 0.842/93.3% (n = 15) for TCF3-PBX1, and 0.535/78.8% (n = 33) for BCR-ABL1. Specific immunophenotypic abnormalities were more prevalent in each genetic subgroup, such as CD38 underexpression, CD58 overexpression, and CD34 overexpression in ETV6-RUNX1, TCF3-PBX1, and BCR-ABL1, respectively. Conclusion: In most follow-up samples, MRD estimates by two methods are in agreement, especially in patients with TCF3-PBX1.http://www.sciencedirect.com/science/article/pii/S0929664616304806B-precursor acute lymphoblastic leukemiaflow cytometryfusion transcriptsminimal residual disease |
| spellingShingle | Ying-Jung Huang Elaine Coustan-Smith Hsiao-Wen Kao Hsi-Che Liu Shih-Hsiang Chen Chih-Cheng Hsiao Chao-Ping Yang Tang-Her Jaing Ting-Chi Yeh Ming-Chung Kuo Chang-Liang Lai Chia-Hui Chang Dario Campana Der-Cherng Liang Lee-Yung Shih Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes Journal of the Formosan Medical Association B-precursor acute lymphoblastic leukemia flow cytometry fusion transcripts minimal residual disease |
| title | Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes |
| title_full | Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes |
| title_fullStr | Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes |
| title_full_unstemmed | Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes |
| title_short | Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes |
| title_sort | concordance of two approaches in monitoring of minimal residual disease in b precursor acute lymphoblastic leukemia fusion transcripts and leukemia associated immunophenotypes |
| topic | B-precursor acute lymphoblastic leukemia flow cytometry fusion transcripts minimal residual disease |
| url | http://www.sciencedirect.com/science/article/pii/S0929664616304806 |
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