Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species

Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species

ABSTRACT The Streptococcus mutans genetic system offers a variety of strategies to rapidly engineer targeted chromosomal mutations. Previously, we reported the first S. mutans negative selection system that functions in a wild-type background. This system utilizes induced sensitivity to the toxic am...

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Bibliographic Details
Main Authors: Lena Li, Madeline Krieger, Hua Qin, Zhengzhong Zou, Jens Kreth, Justin Merritt
Format: Article
Language:English
Published: American Society for Microbiology 2023-06-01
Series:mSphere
Subjects:
markerless mutation
negative selection
counterselection
toxin-antitoxin
xylose
Streptococcus
Online Access:https://journals.asm.org/doi/10.1128/msphere.00682-22

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