Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions
In this work we compare the main changes in protein composition of intermediate filaments due to ionization radiation actions on old and yang rats. The objects of our investigation were 120 rats (60 old and 60 yangs). They were divided on groups in depend on ionization radiation action duration – 1...
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| Format: | Article |
| Language: | Russian |
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Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy
2008-01-01
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| Series: | Морфологія |
| Subjects: | |
| Online Access: | http://www.morphology.dp.ua/_pub/MORPHO-2008-02-02/08rlavio.pdf |
| _version_ | 1811287233384153088 |
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| author | Romanenko L.A. |
| author_facet | Romanenko L.A. |
| author_sort | Romanenko L.A. |
| collection | DOAJ |
| description | In this work we compare the main changes in protein composition of intermediate filaments due to ionization radiation actions on old and yang rats. The objects of our investigation were 120 rats (60 old and 60 yangs). They were divided on groups in depend on ionization radiation action duration – 1 day, 1,2 and 3 week, and the control group. The action of ionization radiation was modeled with the help of “РУМ-17” in the fate 0,00129 Kl/kg. Brains were divided on parts (cortex, cerebellum, gypocamp), homogenized, centrifugised. The quantity of glial and neuronal filaments (NF) was defined by the rocket-lineal immunoelectrophoresis; immunoblotting with specific antibodies was used for qualitative changes defining. After ionization radiation action we observed no age features in protein composition of intermediate filaments. All changes were due to duration of the ionization radiation action. Main changes in neurofilaments were associated with decrease of 210 kDa subunit. Products of degradation (40 - 48 kDa) of fibrillar glial filaments were observed on the 2 week of experiment. There were biphasic changes in glial fibrillar acidic protein in cerebellum and gypocamp of yang and old rats. |
| first_indexed | 2024-04-13T03:15:07Z |
| format | Article |
| id | doaj.art-3d1dd92e0c764d29ad0d643123b4c185 |
| institution | Directory Open Access Journal |
| issn | 1997-9665 |
| language | Russian |
| last_indexed | 2024-04-13T03:15:07Z |
| publishDate | 2008-01-01 |
| publisher | Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy |
| record_format | Article |
| series | Морфологія |
| spelling | doaj.art-3d1dd92e0c764d29ad0d643123b4c1852022-12-22T03:04:56ZrusMinistry of Health of Ukraine. Dnipropetrovsk Medical AcademyМорфологія1997-96652008-01-01226165Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actionsRomanenko L.A.In this work we compare the main changes in protein composition of intermediate filaments due to ionization radiation actions on old and yang rats. The objects of our investigation were 120 rats (60 old and 60 yangs). They were divided on groups in depend on ionization radiation action duration – 1 day, 1,2 and 3 week, and the control group. The action of ionization radiation was modeled with the help of “РУМ-17” in the fate 0,00129 Kl/kg. Brains were divided on parts (cortex, cerebellum, gypocamp), homogenized, centrifugised. The quantity of glial and neuronal filaments (NF) was defined by the rocket-lineal immunoelectrophoresis; immunoblotting with specific antibodies was used for qualitative changes defining. After ionization radiation action we observed no age features in protein composition of intermediate filaments. All changes were due to duration of the ionization radiation action. Main changes in neurofilaments were associated with decrease of 210 kDa subunit. Products of degradation (40 - 48 kDa) of fibrillar glial filaments were observed on the 2 week of experiment. There were biphasic changes in glial fibrillar acidic protein in cerebellum and gypocamp of yang and old rats.http://www.morphology.dp.ua/_pub/MORPHO-2008-02-02/08rlavio.pdfbrainneurofilamentsglial fibrillar acidic proteinionization radiation |
| spellingShingle | Romanenko L.A. Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions Морфологія brain neurofilaments glial fibrillar acidic protein ionization radiation |
| title | Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions |
| title_full | Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions |
| title_fullStr | Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions |
| title_full_unstemmed | Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions |
| title_short | Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions |
| title_sort | age features in protein s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red s brain depend on ionization radiation actions |
| topic | brain neurofilaments glial fibrillar acidic protein ionization radiation |
| url | http://www.morphology.dp.ua/_pub/MORPHO-2008-02-02/08rlavio.pdf |
| work_keys_str_mv | AT romanenkola agefeaturesinproteinscompositionmodificationsoftheneurospecificalandglialintermediatefilamentsindifferentpartsoftheredsbraindependonionizationradiationactions |