Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts

The kinetic behaviour of tomato waste extracts (obtained from six genotypes) and standard antioxidant compounds (ascorbic and caffeic acid) were investigated using 2,2-diphenyl-1-picrylhydrazyl radical test. Based on the time needed for the reaction to reach steady state, the investigated extract...

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Main Authors: Ćetković Gordana S., Čanadanović-Brunet Jasna M., Đilas Sonja M., Savatović Slađana M.
Format: Article
Language:English
Published: Serbian Chemical Society 2012-01-01
Series:Journal of the Serbian Chemical Society
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0352-5139/2012/0352-51391200065S.pdf
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author Ćetković Gordana S.
Čanadanović-Brunet Jasna M.
Đilas Sonja M.
Savatović Slađana M.
author_facet Ćetković Gordana S.
Čanadanović-Brunet Jasna M.
Đilas Sonja M.
Savatović Slađana M.
author_sort Ćetković Gordana S.
collection DOAJ
description The kinetic behaviour of tomato waste extracts (obtained from six genotypes) and standard antioxidant compounds (ascorbic and caffeic acid) were investigated using 2,2-diphenyl-1-picrylhydrazyl radical test. Based on the time needed for the reaction to reach steady state, the investigated extracts show very slow (steady state ≥ 180 min) antiradical behaviour, ascorbic acid act as rapid antioxidant (steady state < 5min) while caffeic acid is rapidintermediate antioxidant (5 min < steady state < 20 min). The efficient concentrations at different kinetic times EC50,t were determined for all extracts, ascorbic and caffeic acid. EC50,t was used as a parameter to screen and compare antiradical activities of food extracts with slow kinetic action. Irrespective of the time considered, the comparison of the EC50,t for extracts showed that the DPPH radicals scavenging activity of extracts decreased in the order of O2 > Knjaz > Bačka > Saint Pierre > Rutgers > Novosadski niski. Tomato waste extracts showed very slow kinetic action, which is probably result of the different kinetic bevaviour of phenolic compounds, present in tomato waste, as well as other antioxidants (vitamins, carotenoids etc).
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spelling doaj.art-3d20115cbd774821ade5b6317d02b0182022-12-22T01:08:49ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392012-01-0177101381138910.2298/JSC120410065SKinetic behaviour of DPPH radical scavenging activity of tomato waste extractsĆetković Gordana S.Čanadanović-Brunet Jasna M.Đilas Sonja M.Savatović Slađana M.The kinetic behaviour of tomato waste extracts (obtained from six genotypes) and standard antioxidant compounds (ascorbic and caffeic acid) were investigated using 2,2-diphenyl-1-picrylhydrazyl radical test. Based on the time needed for the reaction to reach steady state, the investigated extracts show very slow (steady state ≥ 180 min) antiradical behaviour, ascorbic acid act as rapid antioxidant (steady state < 5min) while caffeic acid is rapidintermediate antioxidant (5 min < steady state < 20 min). The efficient concentrations at different kinetic times EC50,t were determined for all extracts, ascorbic and caffeic acid. EC50,t was used as a parameter to screen and compare antiradical activities of food extracts with slow kinetic action. Irrespective of the time considered, the comparison of the EC50,t for extracts showed that the DPPH radicals scavenging activity of extracts decreased in the order of O2 > Knjaz > Bačka > Saint Pierre > Rutgers > Novosadski niski. Tomato waste extracts showed very slow kinetic action, which is probably result of the different kinetic bevaviour of phenolic compounds, present in tomato waste, as well as other antioxidants (vitamins, carotenoids etc).http://www.doiserbia.nb.rs/img/doi/0352-5139/2012/0352-51391200065S.pdftomato wasteDPPH radicalsfree radical scavengerkinetic behaviour
spellingShingle Ćetković Gordana S.
Čanadanović-Brunet Jasna M.
Đilas Sonja M.
Savatović Slađana M.
Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts
Journal of the Serbian Chemical Society
tomato waste
DPPH radicals
free radical scavenger
kinetic behaviour
title Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts
title_full Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts
title_fullStr Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts
title_full_unstemmed Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts
title_short Kinetic behaviour of DPPH radical scavenging activity of tomato waste extracts
title_sort kinetic behaviour of dpph radical scavenging activity of tomato waste extracts
topic tomato waste
DPPH radicals
free radical scavenger
kinetic behaviour
url http://www.doiserbia.nb.rs/img/doi/0352-5139/2012/0352-51391200065S.pdf
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AT đilassonjam kineticbehaviourofdpphradicalscavengingactivityoftomatowasteextracts
AT savatovicslađanam kineticbehaviourofdpphradicalscavengingactivityoftomatowasteextracts