Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction
The role of mycobacterial infection, particularly related to Mycobacterium avium subsp paratuberculosis (Map), in Crohn's disease has long been debated. We developed primer pairs capable of detecting a broad spectrum of mycobacterium and employed them to investigate surgical specimens from pati...
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| Format: | Article |
| Language: | English |
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Elsevier
2006-01-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664609601200 |
| _version_ | 1819297250979872768 |
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| author | Chi-Yuan Tzen Tsu-Yen Wu Chin-Yuan Tzen |
| author_facet | Chi-Yuan Tzen Tsu-Yen Wu Chin-Yuan Tzen |
| author_sort | Chi-Yuan Tzen |
| collection | DOAJ |
| description | The role of mycobacterial infection, particularly related to Mycobacterium avium subsp paratuberculosis (Map), in Crohn's disease has long been debated. We developed primer pairs capable of detecting a broad spectrum of mycobacterium and employed them to investigate surgical specimens from patients with Crohn's disease.
Methods: Pan mycobacterium primers of the 65-kDa heat shock protein gene (Hsp65) were used in a polymerase chain reaction (PCR) to examine 12 surgically-resected, formalin-fixed, paraffin-embedded specimens from 11 patients with Crohn's disease. The DNA sequences of amplicons were aligned with those in GenBank.
Results: Mycobacterial DNA was found in specimens from three of 11 patients. M. mucogenicum was identified in a specimen from one patient and M. tuberculosis in two, but Map was not identified in any.
Conclusion: Hsp65-based PCR can be employed to search for occult mycobacterial infection of the gastrointestinal tract in patients with a diagnosis or suspicion of Crohn's disease. This approach may have a therapeutic implication. |
| first_indexed | 2024-12-24T05:11:02Z |
| format | Article |
| id | doaj.art-3d21674a6edb42289e8deab423d7ab68 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-24T05:11:02Z |
| publishDate | 2006-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-3d21674a6edb42289e8deab423d7ab682022-12-21T17:13:41ZengElsevierJournal of the Formosan Medical Association0929-66462006-01-01105429029810.1016/S0929-6646(09)60120-0Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain ReactionChi-Yuan Tzen0Tsu-Yen Wu1Chin-Yuan Tzen2Department of Pathology, Taipei, TaiwanDepartment of Medical Research, Mackay Memorial Hospital, Taipei, TaiwanDepartment of Pathology, Taipei, TaiwanThe role of mycobacterial infection, particularly related to Mycobacterium avium subsp paratuberculosis (Map), in Crohn's disease has long been debated. We developed primer pairs capable of detecting a broad spectrum of mycobacterium and employed them to investigate surgical specimens from patients with Crohn's disease. Methods: Pan mycobacterium primers of the 65-kDa heat shock protein gene (Hsp65) were used in a polymerase chain reaction (PCR) to examine 12 surgically-resected, formalin-fixed, paraffin-embedded specimens from 11 patients with Crohn's disease. The DNA sequences of amplicons were aligned with those in GenBank. Results: Mycobacterial DNA was found in specimens from three of 11 patients. M. mucogenicum was identified in a specimen from one patient and M. tuberculosis in two, but Map was not identified in any. Conclusion: Hsp65-based PCR can be employed to search for occult mycobacterial infection of the gastrointestinal tract in patients with a diagnosis or suspicion of Crohn's disease. This approach may have a therapeutic implication.http://www.sciencedirect.com/science/article/pii/S0929664609601200Crohn's diseaseheat shock protein geneMycobacterium mucogenicumMycobacterium tuberculosispolymerase chain reaction |
| spellingShingle | Chi-Yuan Tzen Tsu-Yen Wu Chin-Yuan Tzen Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction Journal of the Formosan Medical Association Crohn's disease heat shock protein gene Mycobacterium mucogenicum Mycobacterium tuberculosis polymerase chain reaction |
| title | Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction |
| title_full | Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction |
| title_fullStr | Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction |
| title_full_unstemmed | Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction |
| title_short | Detection of Mycobacteria in Crohn's Disease by a Broad Spectrum Polymerase Chain Reaction |
| title_sort | detection of mycobacteria in crohn s disease by a broad spectrum polymerase chain reaction |
| topic | Crohn's disease heat shock protein gene Mycobacterium mucogenicum Mycobacterium tuberculosis polymerase chain reaction |
| url | http://www.sciencedirect.com/science/article/pii/S0929664609601200 |
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