From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders
Experimental and clinical evidence has demonstrated the potential of probiotic strains in the prevention or treatment of inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). However, there is little data on what the methodology leading to the identification of such strains should be....
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/906 |
| _version_ | 1797604231277969408 |
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| author | Flore Maillard Maëva Meynier Stanislas Mondot Frederic Pepke Chloé Galbert Edgar Torres Maravilla Camille Kropp Harry Sokol Frédéric Antonio Carvalho Elsa Jacouton Sophie Holowacz Philippe Langella Florian Chain Rebeca Martín |
| author_facet | Flore Maillard Maëva Meynier Stanislas Mondot Frederic Pepke Chloé Galbert Edgar Torres Maravilla Camille Kropp Harry Sokol Frédéric Antonio Carvalho Elsa Jacouton Sophie Holowacz Philippe Langella Florian Chain Rebeca Martín |
| author_sort | Flore Maillard |
| collection | DOAJ |
| description | Experimental and clinical evidence has demonstrated the potential of probiotic strains in the prevention or treatment of inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). However, there is little data on what the methodology leading to the identification of such strains should be. In this work, we propose a new flowchart to identify strains with probiotic potential for the management of IBS and IBD, which we tested on a collection of 39 lactic acid bacteria and Bifidobacteria strains. This flowchart included in vitro tests of immunomodulatory properties on intestinal and peripheral blood mononuclear cells (PBMCs), assessment of the barrier-strengthening effect by measuring transepithelial electric resistance (TEER) and quantification of short-chain fatty acids (SCFAs) and aryl hydrocarbon receptor (AhR) agonists produced by the strains. The in vitro results were then combined in a principal component analysis (PCA) to identify strains associated with an anti-inflammatory profile. To validate our flowchart, we tested the two most promising strains identified in the PCA in mouse models of post-infectious IBS or chemically induced colitis to mimic IBD. Our results show that this screening strategy allows the identification of strains with potential beneficial effects on colonic inflammation and colonic hypersensitivity. |
| first_indexed | 2024-03-11T04:43:24Z |
| format | Article |
| id | doaj.art-3d22a5a7efbb478e867ccc09d13dca2c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:24Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3d22a5a7efbb478e867ccc09d13dca2c2023-11-17T20:32:15ZengMDPI AGMicroorganisms2076-26072023-03-0111490610.3390/microorganisms11040906From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal DisordersFlore Maillard0Maëva Meynier1Stanislas Mondot2Frederic Pepke3Chloé Galbert4Edgar Torres Maravilla5Camille Kropp6Harry Sokol7Frédéric Antonio Carvalho8Elsa Jacouton9Sophie Holowacz10Philippe Langella11Florian Chain12Rebeca Martín13INRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceM2iSH, UMR 1071 INSERM, University of Clermont Auvergne, INRAE USC 2018, 63001 Clermont-Ferrand, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINSERM, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Saint Antoine Hospital, Gastroenterology Department, Sorbonne University, 75012 Paris, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceM2iSH, UMR 1071 INSERM, University of Clermont Auvergne, INRAE USC 2018, 63001 Clermont-Ferrand, FrancePiLeJe Laboratoire, 75015 Paris, FrancePiLeJe Laboratoire, 75015 Paris, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, FranceExperimental and clinical evidence has demonstrated the potential of probiotic strains in the prevention or treatment of inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). However, there is little data on what the methodology leading to the identification of such strains should be. In this work, we propose a new flowchart to identify strains with probiotic potential for the management of IBS and IBD, which we tested on a collection of 39 lactic acid bacteria and Bifidobacteria strains. This flowchart included in vitro tests of immunomodulatory properties on intestinal and peripheral blood mononuclear cells (PBMCs), assessment of the barrier-strengthening effect by measuring transepithelial electric resistance (TEER) and quantification of short-chain fatty acids (SCFAs) and aryl hydrocarbon receptor (AhR) agonists produced by the strains. The in vitro results were then combined in a principal component analysis (PCA) to identify strains associated with an anti-inflammatory profile. To validate our flowchart, we tested the two most promising strains identified in the PCA in mouse models of post-infectious IBS or chemically induced colitis to mimic IBD. Our results show that this screening strategy allows the identification of strains with potential beneficial effects on colonic inflammation and colonic hypersensitivity.https://www.mdpi.com/2076-2607/11/4/906probioticscharacterization flow chartIBSIBDlactic acid bacteriabifidobacteria |
| spellingShingle | Flore Maillard Maëva Meynier Stanislas Mondot Frederic Pepke Chloé Galbert Edgar Torres Maravilla Camille Kropp Harry Sokol Frédéric Antonio Carvalho Elsa Jacouton Sophie Holowacz Philippe Langella Florian Chain Rebeca Martín From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders Microorganisms probiotics characterization flow chart IBS IBD lactic acid bacteria bifidobacteria |
| title | From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders |
| title_full | From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders |
| title_fullStr | From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders |
| title_full_unstemmed | From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders |
| title_short | From In Vitro to In Vivo: A Rational Flowchart for the Selection and Characterization of Candidate Probiotic Strains in Intestinal Disorders |
| title_sort | from in vitro to in vivo a rational flowchart for the selection and characterization of candidate probiotic strains in intestinal disorders |
| topic | probiotics characterization flow chart IBS IBD lactic acid bacteria bifidobacteria |
| url | https://www.mdpi.com/2076-2607/11/4/906 |
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