Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus
Chitinase (EC 3.2.1.14) was produced from the culture filtrate of marine-derived Aspergillus terreus and purified by 65% ammonium sulphate precipitation, followed by gel filtration on Sephadex G-100 and DEAE-Sephadex A-50 ion exchange chromatography, with 5.16-fold of purification and specific activ...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-06-01
|
| Series: | Egyptian Journal of Aquatic Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687428516300218 |
| _version_ | 1818981501003366400 |
|---|---|
| author | Aida M. Farag Hanan M. Abd-Elnabey Hassan A.H. Ibrahim Moustafa El-Shenawy |
| author_facet | Aida M. Farag Hanan M. Abd-Elnabey Hassan A.H. Ibrahim Moustafa El-Shenawy |
| author_sort | Aida M. Farag |
| collection | DOAJ |
| description | Chitinase (EC 3.2.1.14) was produced from the culture filtrate of marine-derived Aspergillus terreus and purified by 65% ammonium sulphate precipitation, followed by gel filtration on Sephadex G-100 and DEAE-Sephadex A-50 ion exchange chromatography, with 5.16-fold of purification and specific activity of 182.08 U/mg protein. The molecular weight of the purified chitinase was 60 kDa, determined by a sodium dodecyl sulphate polyacrylamide gel electrophoresis. The optimum pH and temperature of purified chitinase were 5.6 and 50 °C, respectively. The chitinase enzyme was stable from pH 5 to 7.5 and stable up to 70 °C. The effect of activators and inhibitors was studied, Hg+, pb, EDTA, ethanol, methanol and acetone strongly inhibited the enzyme activity, while, metal ions such as Ca2+, Mn2+ and Na2+ highly increased chitinase activity. The purified chitinase produced by A. terreus inhibited the growth of Aspergillus niger, Aspergillus oryzae, Penicillum oxysporium, Rhizocotonia solani, Candida albicans and Fusarium solani, while did not inhibit the growth of Rhizopus oryzae. Moreover, the purified enzyme had antibacterial effects against some pathogenic bacteria such as; Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa, while, it had not any activity against Escherichia coli, Aeromonas hydrophila and Photobacterium damsela. |
| first_indexed | 2024-12-20T17:32:19Z |
| format | Article |
| id | doaj.art-3d26c64c59f84826a1ec9950bf3c086f |
| institution | Directory Open Access Journal |
| issn | 1687-4285 |
| language | English |
| last_indexed | 2024-12-20T17:32:19Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Egyptian Journal of Aquatic Research |
| spelling | doaj.art-3d26c64c59f84826a1ec9950bf3c086f2022-12-21T19:31:21ZengElsevierEgyptian Journal of Aquatic Research1687-42852016-06-0142218519210.1016/j.ejar.2016.04.004Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreusAida M. Farag0Hanan M. Abd-Elnabey1Hassan A.H. Ibrahim2Moustafa El-Shenawy3Marine Biotechnology and Natural Products Laboratory, National Institute of Oceanography and Fisheries, Alexandria, EgyptMicrobiology Laboratory, National Institute of Oceanography and Fisheries, Alexandria, EgyptMicrobiology Laboratory, National Institute of Oceanography and Fisheries, Alexandria, EgyptDept. Food and Environmental Microbiology, National Research Center, Cairo, EgyptChitinase (EC 3.2.1.14) was produced from the culture filtrate of marine-derived Aspergillus terreus and purified by 65% ammonium sulphate precipitation, followed by gel filtration on Sephadex G-100 and DEAE-Sephadex A-50 ion exchange chromatography, with 5.16-fold of purification and specific activity of 182.08 U/mg protein. The molecular weight of the purified chitinase was 60 kDa, determined by a sodium dodecyl sulphate polyacrylamide gel electrophoresis. The optimum pH and temperature of purified chitinase were 5.6 and 50 °C, respectively. The chitinase enzyme was stable from pH 5 to 7.5 and stable up to 70 °C. The effect of activators and inhibitors was studied, Hg+, pb, EDTA, ethanol, methanol and acetone strongly inhibited the enzyme activity, while, metal ions such as Ca2+, Mn2+ and Na2+ highly increased chitinase activity. The purified chitinase produced by A. terreus inhibited the growth of Aspergillus niger, Aspergillus oryzae, Penicillum oxysporium, Rhizocotonia solani, Candida albicans and Fusarium solani, while did not inhibit the growth of Rhizopus oryzae. Moreover, the purified enzyme had antibacterial effects against some pathogenic bacteria such as; Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa, while, it had not any activity against Escherichia coli, Aeromonas hydrophila and Photobacterium damsela.http://www.sciencedirect.com/science/article/pii/S1687428516300218ChitinasePurificationCharacterizationAspergillus terreusAntimicrobial activity |
| spellingShingle | Aida M. Farag Hanan M. Abd-Elnabey Hassan A.H. Ibrahim Moustafa El-Shenawy Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus Egyptian Journal of Aquatic Research Chitinase Purification Characterization Aspergillus terreus Antimicrobial activity |
| title | Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus |
| title_full | Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus |
| title_fullStr | Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus |
| title_full_unstemmed | Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus |
| title_short | Purification, characterization and antimicrobial activity of chitinase from marine-derived Aspergillus terreus |
| title_sort | purification characterization and antimicrobial activity of chitinase from marine derived aspergillus terreus |
| topic | Chitinase Purification Characterization Aspergillus terreus Antimicrobial activity |
| url | http://www.sciencedirect.com/science/article/pii/S1687428516300218 |
| work_keys_str_mv | AT aidamfarag purificationcharacterizationandantimicrobialactivityofchitinasefrommarinederivedaspergillusterreus AT hananmabdelnabey purificationcharacterizationandantimicrobialactivityofchitinasefrommarinederivedaspergillusterreus AT hassanahibrahim purificationcharacterizationandantimicrobialactivityofchitinasefrommarinederivedaspergillusterreus AT moustafaelshenawy purificationcharacterizationandantimicrobialactivityofchitinasefrommarinederivedaspergillusterreus |