Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole
(1) Background: Infections by <i>Candida</i> species represent a serious threat to the health of immunocompromised individuals. Evidence has indicated that nerolidol has significant antifungal properties. Nonetheless, its use is restricted due to a low water solubility and high photosens...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/10/9/194 |
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| author | Camila Fonseca Bezerra José Geraldo de Alencar Júnior Rosilaine de Lima Honorato Antonia Thassya Lucas dos Santos Josefa Carolaine Pereira da Silva Taís Gusmão da Silva Antonio Linkoln Alves Borges Leal Thiago Sampaio de Freitas Thiago Adler Tavares Vieira Janaína Esmeraldo Rocha Débora Lima Sales José Maria Barbosa Filho Gabriela Ribeiro de Sousa Allyson Pontes Pinheiro Jaime Ribeiro-Filho Henrique Douglas Melo Coutinho Maria Flaviana Bezerra Morais-Braga Teresinha Gonçalves da Silva |
| author_facet | Camila Fonseca Bezerra José Geraldo de Alencar Júnior Rosilaine de Lima Honorato Antonia Thassya Lucas dos Santos Josefa Carolaine Pereira da Silva Taís Gusmão da Silva Antonio Linkoln Alves Borges Leal Thiago Sampaio de Freitas Thiago Adler Tavares Vieira Janaína Esmeraldo Rocha Débora Lima Sales José Maria Barbosa Filho Gabriela Ribeiro de Sousa Allyson Pontes Pinheiro Jaime Ribeiro-Filho Henrique Douglas Melo Coutinho Maria Flaviana Bezerra Morais-Braga Teresinha Gonçalves da Silva |
| author_sort | Camila Fonseca Bezerra |
| collection | DOAJ |
| description | (1) Background: Infections by <i>Candida</i> species represent a serious threat to the health of immunocompromised individuals. Evidence has indicated that nerolidol has significant antifungal properties. Nonetheless, its use is restricted due to a low water solubility and high photosensitivity. The incorporation into liposomes may represent an efficient alternative to improve the physicochemical and biopharmaceutical properties of this compound. The present study aimed to characterize the antifungal properties of liposomal nerolidol, alone or in combination with fluconazole. Of note, this is the first study reporting the antifungal activity of liposomal nerolidol and its potentiating effect in association with fluconazole. (2) Methods: The Inhibitory Concentration 50%-IC<sub>50</sub> and minimum fungicide concentrations (MFC) of the substances against <i>Candida albicans</i> (CA), <i>Candida tropicalis</i> (CT), and <i>Candida krusei</i> (CK) were established by subculture in a solid medium. To evaluate the antifungal-enhancing effect, the MFC of fluconazole was determined in the presence or absence of subinhibitory concentrations of nerolidol (free or liposomal). The analysis of fungal dimorphism was performed through optical microscopy and the characterization of liposomes was carried out considering the vesicular size, polydispersion index, and zeta medium potential, in addition to a scanning electron microscopy analysis. (3) Results: The physicochemical characterization revealed that liposomes were obtained as homogenous populations of spherical vesicles. The data obtained in the present study indicate that nerolidol acts as an antifungal agent against <i>Candida albicans</i> and <i>Candida tropicalis</i>, in addition to potentiating (only in the liposomal form) the effect of fluconazole. However, the compound had little inhibitory effect on fungal dimorphism. (4) Conclusions: The incorporation of nerolidol into liposomes improved its antifungal-modulating properties. |
| first_indexed | 2024-03-10T17:06:43Z |
| format | Article |
| id | doaj.art-3d26d201372140069cf29a06d9b0bf47 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T17:06:43Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-3d26d201372140069cf29a06d9b0bf472023-11-20T10:47:41ZengMDPI AGMembranes2077-03752020-08-0110919410.3390/membranes10090194Antifungal Properties of Nerolidol-Containing Liposomes in Association with FluconazoleCamila Fonseca Bezerra0José Geraldo de Alencar Júnior1Rosilaine de Lima Honorato2Antonia Thassya Lucas dos Santos3Josefa Carolaine Pereira da Silva4Taís Gusmão da Silva5Antonio Linkoln Alves Borges Leal6Thiago Sampaio de Freitas7Thiago Adler Tavares Vieira8Janaína Esmeraldo Rocha9Débora Lima Sales10José Maria Barbosa Filho11Gabriela Ribeiro de Sousa12Allyson Pontes Pinheiro13Jaime Ribeiro-Filho14Henrique Douglas Melo Coutinho15Maria Flaviana Bezerra Morais-Braga16Teresinha Gonçalves da Silva17Department of Pharmaceutical Sciences, Federal University of Pernambuco-UFPE, Recife-PE 50670-901, BrazilDepartment of Pharmacy, Federal University of Ceará-UFC, Fortaleza-CE 60020-181, BrazilDepartment of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Pharmacy, Federal University of Paraíba-UFPB, João Pessoa-PB 58051-900, BrazilDepartment of Pharmacy, Federal University of Paraíba-UFPB, João Pessoa-PB 58051-900, BrazilDepartment of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilGonçalo Moniz Institute, Oswaldo Cruz Foundation, Salvador-BA 40296-710, BrazilDepartment of Biological Chemistry, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartment of Biological Sciences, Regional University of Cariri-URCA, Crato-CE 63105-010, BrazilDepartament of Antibiotics, Federal University of Pernambuco-UFPE, Recife-PE 50670-901, Brazil(1) Background: Infections by <i>Candida</i> species represent a serious threat to the health of immunocompromised individuals. Evidence has indicated that nerolidol has significant antifungal properties. Nonetheless, its use is restricted due to a low water solubility and high photosensitivity. The incorporation into liposomes may represent an efficient alternative to improve the physicochemical and biopharmaceutical properties of this compound. The present study aimed to characterize the antifungal properties of liposomal nerolidol, alone or in combination with fluconazole. Of note, this is the first study reporting the antifungal activity of liposomal nerolidol and its potentiating effect in association with fluconazole. (2) Methods: The Inhibitory Concentration 50%-IC<sub>50</sub> and minimum fungicide concentrations (MFC) of the substances against <i>Candida albicans</i> (CA), <i>Candida tropicalis</i> (CT), and <i>Candida krusei</i> (CK) were established by subculture in a solid medium. To evaluate the antifungal-enhancing effect, the MFC of fluconazole was determined in the presence or absence of subinhibitory concentrations of nerolidol (free or liposomal). The analysis of fungal dimorphism was performed through optical microscopy and the characterization of liposomes was carried out considering the vesicular size, polydispersion index, and zeta medium potential, in addition to a scanning electron microscopy analysis. (3) Results: The physicochemical characterization revealed that liposomes were obtained as homogenous populations of spherical vesicles. The data obtained in the present study indicate that nerolidol acts as an antifungal agent against <i>Candida albicans</i> and <i>Candida tropicalis</i>, in addition to potentiating (only in the liposomal form) the effect of fluconazole. However, the compound had little inhibitory effect on fungal dimorphism. (4) Conclusions: The incorporation of nerolidol into liposomes improved its antifungal-modulating properties.https://www.mdpi.com/2077-0375/10/9/194nerolidolliposomesfluconazole<i>candida</i> dimorphismantifungal resistance inhibition |
| spellingShingle | Camila Fonseca Bezerra José Geraldo de Alencar Júnior Rosilaine de Lima Honorato Antonia Thassya Lucas dos Santos Josefa Carolaine Pereira da Silva Taís Gusmão da Silva Antonio Linkoln Alves Borges Leal Thiago Sampaio de Freitas Thiago Adler Tavares Vieira Janaína Esmeraldo Rocha Débora Lima Sales José Maria Barbosa Filho Gabriela Ribeiro de Sousa Allyson Pontes Pinheiro Jaime Ribeiro-Filho Henrique Douglas Melo Coutinho Maria Flaviana Bezerra Morais-Braga Teresinha Gonçalves da Silva Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole Membranes nerolidol liposomes fluconazole <i>candida</i> dimorphism antifungal resistance inhibition |
| title | Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole |
| title_full | Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole |
| title_fullStr | Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole |
| title_full_unstemmed | Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole |
| title_short | Antifungal Properties of Nerolidol-Containing Liposomes in Association with Fluconazole |
| title_sort | antifungal properties of nerolidol containing liposomes in association with fluconazole |
| topic | nerolidol liposomes fluconazole <i>candida</i> dimorphism antifungal resistance inhibition |
| url | https://www.mdpi.com/2077-0375/10/9/194 |
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