糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少
Abstract Purpose Diabetic cardiomyopathy is the leading cause of death in diabetic patients, and the mechanism by which factors other than hyperglycemia contribute to the development of diabetic cardiomyopathy is unknown. Serum small extracellular vesicles (sEVs) carry bioactive proteins or nuclei,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Journal of Diabetes |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1753-0407.13457 |
| _version_ | 1797372710424150016 |
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| author | Wei Ding Xuejuan Zhang Dandan Xiao Wenguang Chang |
| author_facet | Wei Ding Xuejuan Zhang Dandan Xiao Wenguang Chang |
| author_sort | Wei Ding |
| collection | DOAJ |
| description | Abstract Purpose Diabetic cardiomyopathy is the leading cause of death in diabetic patients, and the mechanism by which factors other than hyperglycemia contribute to the development of diabetic cardiomyopathy is unknown. Serum small extracellular vesicles (sEVs) carry bioactive proteins or nuclei, which enter into remote tissues and modulate cell functions. However, in diabetic conditions, the changes of lipids carried by sEVs has not been identified. Our study aims to explore the changes of lipids in sEVs in diabetic patients with cardiovascular disease, we hope to provide new ideas for understanding the role of lipid metabolism in the pathogenesis of diabetic cardiomyopathy. Methods SEVs samples derived from serum of health controls (Ctrl), diabetic patients without cardiovascular diseases (DM), and diabetic patients with cardiovascular diseases (DM‐CAD) were used for lipidomics analysis. Because AC16 cells are also treated with those sEVs to confirm the entrance of cells and effects on insulin sensitivity, a lipidomics analysis on cells was also performed. Results and Conclusions In this study, we found that docosahexaenoic acid (DHA)‐triacylglycerides of sEVs from serums of DM‐CAD patients decreased significantly, and those sEVs could enter into AC16 cells and diminish insulin sensitivity. In addition, DHA‐triacylglycerides were also decreased in cells treated with sEVs from DM‐CAD. Therefore, DHA‐triacylglycerides carried by sEVs may mediate intercellular signaling and be associated with the incidence of diabetic cardiovascular complications. |
| first_indexed | 2024-03-08T18:39:44Z |
| format | Article |
| id | doaj.art-3d280ad57f084bdc9cd454d52f2e0009 |
| institution | Directory Open Access Journal |
| issn | 1753-0393 1753-0407 |
| language | English |
| last_indexed | 2024-03-08T18:39:44Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes |
| spelling | doaj.art-3d280ad57f084bdc9cd454d52f2e00092023-12-29T09:40:20ZengWileyJournal of Diabetes1753-03931753-04072023-12-0115121070108010.1111/1753-0407.13457糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少Wei Ding0Xuejuan Zhang1Dandan Xiao2Wenguang Chang3Department of General Medicine, The Affiliated Hospital, College of Medicine Qingdao University Qingdao ChinaDepartment of General Medicine, The Affiliated Hospital, College of Medicine Qingdao University Qingdao ChinaSchool of Basic Medical Sciences, College of Medicine Qingdao University Qingdao ChinaInstitute for Translational Medicine, The Affiliated Hospital, College of Medicine Qingdao University Qingdao ChinaAbstract Purpose Diabetic cardiomyopathy is the leading cause of death in diabetic patients, and the mechanism by which factors other than hyperglycemia contribute to the development of diabetic cardiomyopathy is unknown. Serum small extracellular vesicles (sEVs) carry bioactive proteins or nuclei, which enter into remote tissues and modulate cell functions. However, in diabetic conditions, the changes of lipids carried by sEVs has not been identified. Our study aims to explore the changes of lipids in sEVs in diabetic patients with cardiovascular disease, we hope to provide new ideas for understanding the role of lipid metabolism in the pathogenesis of diabetic cardiomyopathy. Methods SEVs samples derived from serum of health controls (Ctrl), diabetic patients without cardiovascular diseases (DM), and diabetic patients with cardiovascular diseases (DM‐CAD) were used for lipidomics analysis. Because AC16 cells are also treated with those sEVs to confirm the entrance of cells and effects on insulin sensitivity, a lipidomics analysis on cells was also performed. Results and Conclusions In this study, we found that docosahexaenoic acid (DHA)‐triacylglycerides of sEVs from serums of DM‐CAD patients decreased significantly, and those sEVs could enter into AC16 cells and diminish insulin sensitivity. In addition, DHA‐triacylglycerides were also decreased in cells treated with sEVs from DM‐CAD. Therefore, DHA‐triacylglycerides carried by sEVs may mediate intercellular signaling and be associated with the incidence of diabetic cardiovascular complications.https://doi.org/10.1111/1753-0407.13457心脏功能障碍DHA细胞外囊泡胰岛素抵抗三酰甘油 |
| spellingShingle | Wei Ding Xuejuan Zhang Dandan Xiao Wenguang Chang 糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少 Journal of Diabetes 心脏功能障碍 DHA 细胞外囊泡 胰岛素抵抗 三酰甘油 |
| title | 糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少 |
| title_full | 糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少 |
| title_fullStr | 糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少 |
| title_full_unstemmed | 糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少 |
| title_short | 糖尿病合并心脏功能障碍患者细胞外小囊泡中n‐3 DHA 富集的三酰甘油减少 |
| title_sort | 糖尿病合并心脏功能障碍患者细胞外小囊泡中n 3 dha 富集的三酰甘油减少 |
| topic | 心脏功能障碍 DHA 细胞外囊泡 胰岛素抵抗 三酰甘油 |
| url | https://doi.org/10.1111/1753-0407.13457 |
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