Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway
Osteoarthritis (OA), a chronic disease featured by severe pain and persistent inflammation, significantly affects human health and lowers the quality of life. Nuciferine (Nuf) is major alkaloids abundant in lotus leaf and has various biological activities. In current study, we aimed to explored the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621003315 |
| _version_ | 1819137754871627776 |
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| author | Haiyan Wen Siqi Zhou Jianping Li Fei Peng Xiaotao Han Haohuan Li |
| author_facet | Haiyan Wen Siqi Zhou Jianping Li Fei Peng Xiaotao Han Haohuan Li |
| author_sort | Haiyan Wen |
| collection | DOAJ |
| description | Osteoarthritis (OA), a chronic disease featured by severe pain and persistent inflammation, significantly affects human health and lowers the quality of life. Nuciferine (Nuf) is major alkaloids abundant in lotus leaf and has various biological activities. In current study, we aimed to explored the protective effect of Nuf against OA and its underlying mechanism in vitro and in vivo. It was found that Nuf alleviated the degradation of extracellular matrix (ECM) under the stimulation of IL-1β and inhibited the production of inflammation cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in vitro. Meanwhile, it was demonstrated that Nuf suppressed the activation of PI3K/Akt/NF-κB signaling pathway induced by IL-1β. Besides, there was favorable binding ability between Nuf and PI3K. Nuf significantly alleviated the development of OA in vivo in the rat OA model established by anterior cruciate ligament transaction (ACLT). Together, our results indicated that Nuf attenuates the progression of OA by targeting PI3K/Akt/NF-κB signaling pathway, which makes Nuf as a promising candidate for the treatment of OA. |
| first_indexed | 2024-12-22T10:55:54Z |
| format | Article |
| id | doaj.art-3d2a27e74aa548c0a9b1800a306400bd |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-22T10:55:54Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-3d2a27e74aa548c0a9b1800a306400bd2022-12-21T18:28:36ZengElsevierJournal of Functional Foods1756-46462021-11-0186104682Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathwayHaiyan Wen0Siqi Zhou1Jianping Li2Fei Peng3Xiaotao Han4Haohuan Li5Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, PR ChinaDepartment of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaDepartment of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaDepartment of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, PR ChinaWuhan National High Magnetic Field Center, Huazhong University of Science and Technology, Wuhan 430074, PR China; State Key Laboratory of Advanced Electromagnetic Engineering and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR ChinaDepartment of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Corresponding authors.Osteoarthritis (OA), a chronic disease featured by severe pain and persistent inflammation, significantly affects human health and lowers the quality of life. Nuciferine (Nuf) is major alkaloids abundant in lotus leaf and has various biological activities. In current study, we aimed to explored the protective effect of Nuf against OA and its underlying mechanism in vitro and in vivo. It was found that Nuf alleviated the degradation of extracellular matrix (ECM) under the stimulation of IL-1β and inhibited the production of inflammation cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in vitro. Meanwhile, it was demonstrated that Nuf suppressed the activation of PI3K/Akt/NF-κB signaling pathway induced by IL-1β. Besides, there was favorable binding ability between Nuf and PI3K. Nuf significantly alleviated the development of OA in vivo in the rat OA model established by anterior cruciate ligament transaction (ACLT). Together, our results indicated that Nuf attenuates the progression of OA by targeting PI3K/Akt/NF-κB signaling pathway, which makes Nuf as a promising candidate for the treatment of OA.http://www.sciencedirect.com/science/article/pii/S1756464621003315OsteoarthritisNuciferineChondrocytesInflammationNF-κBPI3K |
| spellingShingle | Haiyan Wen Siqi Zhou Jianping Li Fei Peng Xiaotao Han Haohuan Li Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway Journal of Functional Foods Osteoarthritis Nuciferine Chondrocytes Inflammation NF-κB PI3K |
| title | Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway |
| title_full | Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway |
| title_fullStr | Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway |
| title_full_unstemmed | Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway |
| title_short | Nuciferine attenuates the progression of osteoarthritis by targeting PI3K/Akt/NF-κB signaling pathway |
| title_sort | nuciferine attenuates the progression of osteoarthritis by targeting pi3k akt nf κb signaling pathway |
| topic | Osteoarthritis Nuciferine Chondrocytes Inflammation NF-κB PI3K |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621003315 |
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