Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda
Abstract There are 13 globally recognized rubella virus genotypes of which only 2 (1E and 2B) have been detected recently. The largest percentage of all reported rubella virus sequences come from China and Japan with Africa reporting limited data. In a bid to address the lack of rubella genotype dat...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-023-06499-5 |
| _version_ | 1827635689030680576 |
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| author | Phionah Tushabe Barnabas Bakamutumaho James Peter Eliku Molly Birungi Francis Aine Prossy Namuwulya Henry Bukenya Immaculate Ampeire Annet Kisakye Charles R. Byabamazima Josephine Bwogi |
| author_facet | Phionah Tushabe Barnabas Bakamutumaho James Peter Eliku Molly Birungi Francis Aine Prossy Namuwulya Henry Bukenya Immaculate Ampeire Annet Kisakye Charles R. Byabamazima Josephine Bwogi |
| author_sort | Phionah Tushabe |
| collection | DOAJ |
| description | Abstract There are 13 globally recognized rubella virus genotypes of which only 2 (1E and 2B) have been detected recently. The largest percentage of all reported rubella virus sequences come from China and Japan with Africa reporting limited data. In a bid to address the lack of rubella genotype data in Uganda and the World Health Organization Africa region, we sought to characterize rubella viruses retrospectively using sera collected from suspected measles patients that turned out rubella IgM positive. Seven sequences belonging to genotype 2B sub-lineage 2B-L2c were obtained. These sequences clustered with other genotype 2B sequences previously reported from Uganda. None of the other genotypes (1E and 1G) reported from Uganda in the earlier years were detected. In addition, none of the sequences were obtained after the introduction of the measles-rubella containing vaccine. The above highlight the need for continuous rubella virological surveillance to confirm interruption of endemic rubella genotype circulation. |
| first_indexed | 2024-03-09T15:31:02Z |
| format | Article |
| id | doaj.art-3d2abd8729074540ac2cf34d9e0076ca |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-09T15:31:02Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-3d2abd8729074540ac2cf34d9e0076ca2023-11-26T12:14:25ZengBMCBMC Research Notes1756-05002023-09-011611610.1186/s13104-023-06499-5Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in UgandaPhionah Tushabe0Barnabas Bakamutumaho1James Peter Eliku2Molly Birungi3Francis Aine4Prossy Namuwulya5Henry Bukenya6Immaculate Ampeire7Annet Kisakye8Charles R. Byabamazima9Josephine Bwogi10EPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteEPI-Laboratory, Uganda Virus Research InstituteAbstract There are 13 globally recognized rubella virus genotypes of which only 2 (1E and 2B) have been detected recently. The largest percentage of all reported rubella virus sequences come from China and Japan with Africa reporting limited data. In a bid to address the lack of rubella genotype data in Uganda and the World Health Organization Africa region, we sought to characterize rubella viruses retrospectively using sera collected from suspected measles patients that turned out rubella IgM positive. Seven sequences belonging to genotype 2B sub-lineage 2B-L2c were obtained. These sequences clustered with other genotype 2B sequences previously reported from Uganda. None of the other genotypes (1E and 1G) reported from Uganda in the earlier years were detected. In addition, none of the sequences were obtained after the introduction of the measles-rubella containing vaccine. The above highlight the need for continuous rubella virological surveillance to confirm interruption of endemic rubella genotype circulation.https://doi.org/10.1186/s13104-023-06499-5RubellaCongenital Rubella SyndromeGenotypesEndemicSera |
| spellingShingle | Phionah Tushabe Barnabas Bakamutumaho James Peter Eliku Molly Birungi Francis Aine Prossy Namuwulya Henry Bukenya Immaculate Ampeire Annet Kisakye Charles R. Byabamazima Josephine Bwogi Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda BMC Research Notes Rubella Congenital Rubella Syndrome Genotypes Endemic Sera |
| title | Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda |
| title_full | Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda |
| title_fullStr | Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda |
| title_full_unstemmed | Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda |
| title_short | Rubella virus genotype 2B endemicity and related utility of serum-based molecular characterization in Uganda |
| title_sort | rubella virus genotype 2b endemicity and related utility of serum based molecular characterization in uganda |
| topic | Rubella Congenital Rubella Syndrome Genotypes Endemic Sera |
| url | https://doi.org/10.1186/s13104-023-06499-5 |
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