Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico
Introduction and objectives: Cases of viral hepatitis reported in Mexico are typically identified as hepatitis A, B and C. However, unspecified cases are reported annually. Hepatitis E virus (HEV) is an emergent agent that causes a self-limiting infection that can evolve to chronic in immunosuppress...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-05-01
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| Series: | Annals of Hepatology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1665268119322847 |
| _version_ | 1819107301359878144 |
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| author | Tayde López-Santaella Teresa Álvarez y Muñoz Mara Medeiros-Domingo Sarbelio Moreno-Espinosa Alejandra Consuelo-Sánchez Onofre Muñoz-Hernández Rosa Elena Sarmiento-Silva Alicia Sotomayor-González María Elena Trujillo-Ortega Montserrat Elemi García-Hernández Blanca Itzel Taboada-Ramírez Francisco Arenas-Huertero |
| author_facet | Tayde López-Santaella Teresa Álvarez y Muñoz Mara Medeiros-Domingo Sarbelio Moreno-Espinosa Alejandra Consuelo-Sánchez Onofre Muñoz-Hernández Rosa Elena Sarmiento-Silva Alicia Sotomayor-González María Elena Trujillo-Ortega Montserrat Elemi García-Hernández Blanca Itzel Taboada-Ramírez Francisco Arenas-Huertero |
| author_sort | Tayde López-Santaella |
| collection | DOAJ |
| description | Introduction and objectives: Cases of viral hepatitis reported in Mexico are typically identified as hepatitis A, B and C. However, unspecified cases are reported annually. Hepatitis E virus (HEV) is an emergent agent that causes a self-limiting infection that can evolve to chronic in immunosuppressed individuals. In Mexico, HEV genotype 2 is considered endemic, though it's the prevalence is not well known. Therefore, the present study was designed to determine the prevalence of HEV among patients at the “Hospital Infantil de Mexico Federico Gomez”. Materials and methods: The study included 99 patients, anti-HEV antibody (IgG and IgM) were detected by indirect ELISA and viral genome was identified using RT-PCR technique. Two PCR products of positive cases were sequenced. Results: ELISA results were positive in 3% and 6%, for IgG and IgM respectively, 54.5% prevalence was found by PCR. Low lymphocyte count (p < 0.05) and malnutrition (p < 0.005) were significant factors for high PCR prevalence and could increase the possibility of infection. Two samples were sequenced and confirmed the presence of HEV genotype 3. Conclusions: This report reveals the incidence of HEV in pediatric patients in Mexico. Moreover, the identification of HEV genotype 3 in human samples suggests a potential zoonotic risk that requires further research. |
| first_indexed | 2024-12-22T02:51:52Z |
| format | Article |
| id | doaj.art-3d2ac2737ed4412281a022b882c59d19 |
| institution | Directory Open Access Journal |
| issn | 1665-2681 |
| language | English |
| last_indexed | 2024-12-22T02:51:52Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Annals of Hepatology |
| spelling | doaj.art-3d2ac2737ed4412281a022b882c59d192022-12-21T18:41:22ZengElsevierAnnals of Hepatology1665-26812020-05-01193295301Serological and molecular study of Hepatitis E virus in pediatric patients in MexicoTayde López-Santaella0Teresa Álvarez y Muñoz1Mara Medeiros-Domingo2Sarbelio Moreno-Espinosa3Alejandra Consuelo-Sánchez4Onofre Muñoz-Hernández5Rosa Elena Sarmiento-Silva6Alicia Sotomayor-González7María Elena Trujillo-Ortega8Montserrat Elemi García-Hernández9Blanca Itzel Taboada-Ramírez10Francisco Arenas-Huertero11Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoLaboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoServicio de Nefrología, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoServicio de Infectología, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoServicio de Gastroenterología, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoDirección de Investigación, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, Mexico; Corresponding author.Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, MexicoDepartamento de Medicina y Zootecnia de Cerdos, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, MexicoDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México, MexicoDepartamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mor. MexicoLaboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México, MexicoIntroduction and objectives: Cases of viral hepatitis reported in Mexico are typically identified as hepatitis A, B and C. However, unspecified cases are reported annually. Hepatitis E virus (HEV) is an emergent agent that causes a self-limiting infection that can evolve to chronic in immunosuppressed individuals. In Mexico, HEV genotype 2 is considered endemic, though it's the prevalence is not well known. Therefore, the present study was designed to determine the prevalence of HEV among patients at the “Hospital Infantil de Mexico Federico Gomez”. Materials and methods: The study included 99 patients, anti-HEV antibody (IgG and IgM) were detected by indirect ELISA and viral genome was identified using RT-PCR technique. Two PCR products of positive cases were sequenced. Results: ELISA results were positive in 3% and 6%, for IgG and IgM respectively, 54.5% prevalence was found by PCR. Low lymphocyte count (p < 0.05) and malnutrition (p < 0.005) were significant factors for high PCR prevalence and could increase the possibility of infection. Two samples were sequenced and confirmed the presence of HEV genotype 3. Conclusions: This report reveals the incidence of HEV in pediatric patients in Mexico. Moreover, the identification of HEV genotype 3 in human samples suggests a potential zoonotic risk that requires further research.http://www.sciencedirect.com/science/article/pii/S1665268119322847VirusHepatitisInfectious diseaseSerologyPediatric patientsHEV |
| spellingShingle | Tayde López-Santaella Teresa Álvarez y Muñoz Mara Medeiros-Domingo Sarbelio Moreno-Espinosa Alejandra Consuelo-Sánchez Onofre Muñoz-Hernández Rosa Elena Sarmiento-Silva Alicia Sotomayor-González María Elena Trujillo-Ortega Montserrat Elemi García-Hernández Blanca Itzel Taboada-Ramírez Francisco Arenas-Huertero Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico Annals of Hepatology Virus Hepatitis Infectious disease Serology Pediatric patients HEV |
| title | Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico |
| title_full | Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico |
| title_fullStr | Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico |
| title_full_unstemmed | Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico |
| title_short | Serological and molecular study of Hepatitis E virus in pediatric patients in Mexico |
| title_sort | serological and molecular study of hepatitis e virus in pediatric patients in mexico |
| topic | Virus Hepatitis Infectious disease Serology Pediatric patients HEV |
| url | http://www.sciencedirect.com/science/article/pii/S1665268119322847 |
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