β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells
The β-catenin transcriptional coregulator is involved in various biological and pathological processes; however, its requirements in hematopoietic cells remain controversial. We re-targeted the Ctnnb1 gene locus to generate a true β-catenin-null mutant mouse strain. Ablation of β-catenin alone, or i...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/55360 |
| _version_ | 1828380240351592448 |
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| author | Xin Zhao Peng Shao Kexin Gai Fengyin Li Qiang Shan Hai-Hui Xue |
| author_facet | Xin Zhao Peng Shao Kexin Gai Fengyin Li Qiang Shan Hai-Hui Xue |
| author_sort | Xin Zhao |
| collection | DOAJ |
| description | The β-catenin transcriptional coregulator is involved in various biological and pathological processes; however, its requirements in hematopoietic cells remain controversial. We re-targeted the Ctnnb1 gene locus to generate a true β-catenin-null mutant mouse strain. Ablation of β-catenin alone, or in combination with its homologue γ-catenin, did not affect thymocyte maturation, survival or proliferation. Deficiency in β/γ-catenin did not detectably affect differentiation of CD4+T follicular helper cells or that of effector and memory CD8+ cytotoxic cells in response to acute viral infection. In an MLL-AF9 AML mouse model, genetic deletion of β-catenin, or even all four Tcf/Lef family transcription factors that interact with β-catenin, did not affect AML onset in primary recipients, or the ability of leukemic stem cells (LSCs) in propagating AML in secondary recipients. Our data thus clarify on a long-standing controversy and indicate that β-catenin is dispensable for T cells and AML LSCs. |
| first_indexed | 2024-12-10T03:53:49Z |
| format | Article |
| id | doaj.art-3d2af85808994f9e9711d5ce3b08965b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:53:49Z |
| publishDate | 2020-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3d2af85808994f9e9711d5ce3b08965b2022-12-22T02:03:11ZengeLife Sciences Publications LtdeLife2050-084X2020-08-01910.7554/eLife.55360β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cellsXin Zhao0Peng Shao1Kexin Gai2Fengyin Li3Qiang Shan4Hai-Hui Xue5https://orcid.org/0000-0002-9163-7669Center for Discovery and Innovation, Hackensack University Medical Center, Nutley, United StatesDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, United StatesCenter for Discovery and Innovation, Hackensack University Medical Center, Nutley, United StatesHefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, ChinaCenter for Discovery and Innovation, Hackensack University Medical Center, Nutley, United StatesCenter for Discovery and Innovation, Hackensack University Medical Center, Nutley, United States; New Jersey Veterans Affairs Health Care System, East Orange, United StatesThe β-catenin transcriptional coregulator is involved in various biological and pathological processes; however, its requirements in hematopoietic cells remain controversial. We re-targeted the Ctnnb1 gene locus to generate a true β-catenin-null mutant mouse strain. Ablation of β-catenin alone, or in combination with its homologue γ-catenin, did not affect thymocyte maturation, survival or proliferation. Deficiency in β/γ-catenin did not detectably affect differentiation of CD4+T follicular helper cells or that of effector and memory CD8+ cytotoxic cells in response to acute viral infection. In an MLL-AF9 AML mouse model, genetic deletion of β-catenin, or even all four Tcf/Lef family transcription factors that interact with β-catenin, did not affect AML onset in primary recipients, or the ability of leukemic stem cells (LSCs) in propagating AML in secondary recipients. Our data thus clarify on a long-standing controversy and indicate that β-catenin is dispensable for T cells and AML LSCs.https://elifesciences.org/articles/55360beta-cateningamma-cateninTcf/LefT lymphocytesacute myeloid leukemialeukemic stem cells |
| spellingShingle | Xin Zhao Peng Shao Kexin Gai Fengyin Li Qiang Shan Hai-Hui Xue β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells eLife beta-catenin gamma-catenin Tcf/Lef T lymphocytes acute myeloid leukemia leukemic stem cells |
| title | β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells |
| title_full | β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells |
| title_fullStr | β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells |
| title_full_unstemmed | β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells |
| title_short | β-catenin and γ-catenin are dispensable for T lymphocytes and AML leukemic stem cells |
| title_sort | β catenin and γ catenin are dispensable for t lymphocytes and aml leukemic stem cells |
| topic | beta-catenin gamma-catenin Tcf/Lef T lymphocytes acute myeloid leukemia leukemic stem cells |
| url | https://elifesciences.org/articles/55360 |
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