Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana
This study assessed the aflatoxin contamination and the presence of fungi in three maize varieties (Obatanpa, Abontem and Aburohemaa) stored using different storage methods namely storage in hermetic bags, woven polypropylene sacks and local crib in the Nkoranza-South district of the Brong-Ahafo reg...
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Julius Kühn-Institut
2018-11-01
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| Online Access: | https://www.openagrar.de/receive/openagrar_mods_00078464 |
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| author | Benson-Obour, Robert Lartey, Michael Cornelius, William Agyel-Ohemeng, James Opare, Phyllis Cinquanta, Luciano Obeng-Ofori, Daniel |
| author_facet | Benson-Obour, Robert Lartey, Michael Cornelius, William Agyel-Ohemeng, James Opare, Phyllis Cinquanta, Luciano Obeng-Ofori, Daniel |
| author_sort | Benson-Obour, Robert |
| collection | DOAJ |
| description | This study assessed the aflatoxin contamination and the presence of fungi in three maize varieties (Obatanpa, Abontem and Aburohemaa) stored using different storage methods namely storage in hermetic bags, woven polypropylene sacks and local crib in the Nkoranza-South district of the Brong-Ahafo region of Ghana. A factorial design arrangement was laid out in a randomized complete block design (RCBD). The isolation and identification of fungal pathogens associated with maize samples before and after storage were carried out on potato dextrose agar (PDA). Total flatoxin levels in the three maize varieties was determined by the use of enzyme-linked immunosorbent assay (ELISA) at 450 nm wavelength. Six fungi species were identified in the maize namely: Aspergillus flavus, Penicillium sp, Fusarium sp., Lasiodiplodia theobromae, Colletotrichum gleosporioides and Rhizopus. Before storage, Abontem variety recorded significantly higher (p < 0.05) total aflatoxin levels (113.56 ppb) compared to Obatanpa (2.91 ppb) and Aburohemaa (2.96 ppb). Maize samples stored in the polypropylene sack established significantly higher (p < 0.05) total aflatoxin levels of 82.9 ppb compared to hermetic bags (48.9 ppb) and local crib (48.9 ppb) after storage for six months. Aflatoxin levels under the interactive effect of variety and storage method was significant (p < 0.05). Overall storage of maize in hermetic bags significantly reduced aflatoxin levels hence the need to encourage maize farmers and traders to adopt hermetic bag storage technology. |
| first_indexed | 2024-03-08T15:29:16Z |
| format | Article |
| id | doaj.art-3d2b3a41122f4e50b7e8e581900deeae |
| institution | Directory Open Access Journal |
| issn | 1868-9892 2199-921X |
| language | deu |
| last_indexed | 2024-03-08T15:29:16Z |
| publishDate | 2018-11-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Julius-Kühn-Archiv |
| spelling | doaj.art-3d2b3a41122f4e50b7e8e581900deeae2024-01-10T06:40:11ZdeuJulius Kühn-InstitutJulius-Kühn-Archiv1868-98922199-921X2018-11-0146321091109810.5073/jka.2018.463.243Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of GhanaBenson-Obour, Robert0Lartey, Michael1Cornelius, William2Agyel-Ohemeng, James3Opare, Phyllis4Cinquanta, Luciano5Obeng-Ofori, Daniel 6Department of Crop Science, School of Agriculture, University of Ghana, Legon, Accra, GhanaDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Ghana, Legon, Accra, Ghana GhanaDepartment of Crop Science, School of Agriculture, University of Ghana, Legon, Accra, GhanaDepartment of Ecotourism, Recreation and Hospitality, School of Natural Resources, University of Energy and Natural Resources, Sunyani, GhanaDepartment of Languages and General Studies, School of Natural Resources, University of Energy and Natural Resources, Sunyani, GhanaDepartment of Agricultural, Environmental and Food Science, University of Molise, Campobasso, ItalyDepartment of Horticulture and Crop Production, School of Agriculture and Technology, University of Energy and Natural Resources, Sunyani, GhanaThis study assessed the aflatoxin contamination and the presence of fungi in three maize varieties (Obatanpa, Abontem and Aburohemaa) stored using different storage methods namely storage in hermetic bags, woven polypropylene sacks and local crib in the Nkoranza-South district of the Brong-Ahafo region of Ghana. A factorial design arrangement was laid out in a randomized complete block design (RCBD). The isolation and identification of fungal pathogens associated with maize samples before and after storage were carried out on potato dextrose agar (PDA). Total flatoxin levels in the three maize varieties was determined by the use of enzyme-linked immunosorbent assay (ELISA) at 450 nm wavelength. Six fungi species were identified in the maize namely: Aspergillus flavus, Penicillium sp, Fusarium sp., Lasiodiplodia theobromae, Colletotrichum gleosporioides and Rhizopus. Before storage, Abontem variety recorded significantly higher (p < 0.05) total aflatoxin levels (113.56 ppb) compared to Obatanpa (2.91 ppb) and Aburohemaa (2.96 ppb). Maize samples stored in the polypropylene sack established significantly higher (p < 0.05) total aflatoxin levels of 82.9 ppb compared to hermetic bags (48.9 ppb) and local crib (48.9 ppb) after storage for six months. Aflatoxin levels under the interactive effect of variety and storage method was significant (p < 0.05). Overall storage of maize in hermetic bags significantly reduced aflatoxin levels hence the need to encourage maize farmers and traders to adopt hermetic bag storage technology.https://www.openagrar.de/receive/openagrar_mods_00078464aflatoxinfungimaize varietiesobatanpaabontemaburohemaahermetic bagpolypropylene sacklocal crib |
| spellingShingle | Benson-Obour, Robert Lartey, Michael Cornelius, William Agyel-Ohemeng, James Opare, Phyllis Cinquanta, Luciano Obeng-Ofori, Daniel Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana Julius-Kühn-Archiv aflatoxin fungi maize varieties obatanpa abontem aburohemaa hermetic bag polypropylene sack local crib |
| title | Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana |
| title_full | Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana |
| title_fullStr | Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana |
| title_full_unstemmed | Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana |
| title_short | Evaluation of aflatoxin contamination of stored maize in the Brong-Ahafo region of Ghana |
| title_sort | evaluation of aflatoxin contamination of stored maize in the brong ahafo region of ghana |
| topic | aflatoxin fungi maize varieties obatanpa abontem aburohemaa hermetic bag polypropylene sack local crib |
| url | https://www.openagrar.de/receive/openagrar_mods_00078464 |
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