Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection
<p>Abstract</p> <p>Background</p> <p>This study was conducted to <b>e</b>xamine, <it>in vitro </it>, the effect of soluble egg antigen (SEA) of <it>S. haematobium</it> on intracellular HCV RNA load in peripheral mononuclear cells (PBM...
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BMC
2006-06-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/6/91 |
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| author | Tabll Ashraf A Omran Moataza H Youssef Samar S El-Awady Mostafa K El Garf Wael T Salem Ahmed M |
| author_facet | Tabll Ashraf A Omran Moataza H Youssef Samar S El-Awady Mostafa K El Garf Wael T Salem Ahmed M |
| author_sort | Tabll Ashraf A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>This study was conducted to <b>e</b>xamine, <it>in vitro </it>, the effect of soluble egg antigen (SEA) of <it>S. haematobium</it> on intracellular HCV RNA load in peripheral mononuclear cells (PBMC) as well as on cell proliferation in patients with chronic HCV infection.</p> <p>Methods</p> <p>PBMC from 26 patients with chronic HCV infection were cultured for 72 hours in presence and absence of 50 μg SEA/ml medium. Intracellular HCV RNA quantification of plus and minus strands was assessed before and after stimulation. PBMC from five healthy subjects were cultured for 7 days, flow cytometric analysis of DNA content was used to assess the mitogenic effect of SEA on PBMC proliferation compared to phytoheamaglutinine (PHA).</p> <p>Results</p> <p>Quantification of the intracellular viral load showed increased copy number/cell of both or either viral strands after induction with SEA in 18 of 26 patients (69.2%) thus indicating stimulation of viral replication. Flow cytometric analysis showed that mean ± S.D. of percent values of cell proliferation was induced from 3.2 ± 1.5% in un-stimulated cells to 16.7 ± 2.5 % and 16.84 ± 1.7 % in cells stimulated with PHA and SEA respectively.</p> <p>Conclusion</p> <p>the present study supports earlier reports on SEA proliferative activity on PBMC and provides a strong evidence that the higher morbidity observed in patients co-infected with schistosomiasis and HCV is related, at least in part, to direct stimulation of viral replication by SEA.</p> |
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| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T11:19:44Z |
| publishDate | 2006-06-01 |
| publisher | BMC |
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| series | BMC Infectious Diseases |
| spelling | doaj.art-3d2db40124624fe98578f8a45e6fd17e2022-12-22T02:48:51ZengBMCBMC Infectious Diseases1471-23342006-06-01619110.1186/1471-2334-6-91Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infectionTabll Ashraf AOmran Moataza HYoussef Samar SEl-Awady Mostafa KEl Garf Wael TSalem Ahmed M<p>Abstract</p> <p>Background</p> <p>This study was conducted to <b>e</b>xamine, <it>in vitro </it>, the effect of soluble egg antigen (SEA) of <it>S. haematobium</it> on intracellular HCV RNA load in peripheral mononuclear cells (PBMC) as well as on cell proliferation in patients with chronic HCV infection.</p> <p>Methods</p> <p>PBMC from 26 patients with chronic HCV infection were cultured for 72 hours in presence and absence of 50 μg SEA/ml medium. Intracellular HCV RNA quantification of plus and minus strands was assessed before and after stimulation. PBMC from five healthy subjects were cultured for 7 days, flow cytometric analysis of DNA content was used to assess the mitogenic effect of SEA on PBMC proliferation compared to phytoheamaglutinine (PHA).</p> <p>Results</p> <p>Quantification of the intracellular viral load showed increased copy number/cell of both or either viral strands after induction with SEA in 18 of 26 patients (69.2%) thus indicating stimulation of viral replication. Flow cytometric analysis showed that mean ± S.D. of percent values of cell proliferation was induced from 3.2 ± 1.5% in un-stimulated cells to 16.7 ± 2.5 % and 16.84 ± 1.7 % in cells stimulated with PHA and SEA respectively.</p> <p>Conclusion</p> <p>the present study supports earlier reports on SEA proliferative activity on PBMC and provides a strong evidence that the higher morbidity observed in patients co-infected with schistosomiasis and HCV is related, at least in part, to direct stimulation of viral replication by SEA.</p>http://www.biomedcentral.com/1471-2334/6/91 |
| spellingShingle | Tabll Ashraf A Omran Moataza H Youssef Samar S El-Awady Mostafa K El Garf Wael T Salem Ahmed M Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection BMC Infectious Diseases |
| title | Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection |
| title_full | Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection |
| title_fullStr | Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection |
| title_full_unstemmed | Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection |
| title_short | Soluble egg antigen of <it>Schistosoma Haematobium </it>induces HCV replication in PBMC from patients with chronic HCV infection |
| title_sort | soluble egg antigen of it schistosoma haematobium it induces hcv replication in pbmc from patients with chronic hcv infection |
| url | http://www.biomedcentral.com/1471-2334/6/91 |
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