Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.)
Abstract Background Thermo-sensitive male-sterility based on Aegilops kotschyi cytoplasm (K-TCMS) plays an important role in hybrid wheat breeding. This has important possible applications in two-line hybrid wheat breeding but the genetic basis and molecular regulation mechanism related to fertility...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1824-9 |
| _version_ | 1819207353361235968 |
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| author | Sha Li Zihan Liu Yulin Jia Jiali Ye Xuetong Yang Lingli Zhang Xiyue Song |
| author_facet | Sha Li Zihan Liu Yulin Jia Jiali Ye Xuetong Yang Lingli Zhang Xiyue Song |
| author_sort | Sha Li |
| collection | DOAJ |
| description | Abstract Background Thermo-sensitive male-sterility based on Aegilops kotschyi cytoplasm (K-TCMS) plays an important role in hybrid wheat breeding. This has important possible applications in two-line hybrid wheat breeding but the genetic basis and molecular regulation mechanism related to fertility restoration are poorly understood. In this study, comparative transcriptome profiling based on RNA sequencing was conducted for two near-isogenic lines comprising KTM3315R and its sterile counterpart KTM3315A, a total of six samples (3 repetitions per group), in order to identify fertility restoration genes and their metabolic pathways. Results In total, 2642 significant differentially expressed genes (DEGs) were detected, among which 1238 were down-regulated and 1404 were up-regulated in fertile anthers. Functional annotation enrichment analysis identified important pathways related to fertility restoration, such as carbohydrate metabolism, phenylpropanoid metabolism and biosynthesis, as well as candidate genes encoding pectin methylesterase and flavanone 3-hydroxylase. Moreover, transcription factor analysis showed that a large number of DEGs were mainly involved with the WRKY, bHLH, and MYB transcription factor families. Determination of total soluble sugar and flavonoid contents demonstrated that important metabolic pathways and candidate genes are associated with fertility restoration. Twelve DEGs were selected and detected by quantitative reverse-transcribed PCR, and the results indicated that the transcriptome sequencing results were reliable. Conclusions Our results indicate that identified DEGs were related to the fertility restoration and they proved to be crucial in Aegilops kotschyi cytoplasm. These findings also provide a basis for exploring the molecular regulation mechanism associated with wheat fertility restoration as well as screening and cloning related genes. |
| first_indexed | 2024-12-23T05:22:09Z |
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| id | doaj.art-3d2f06fa395a474d886755ebd8d421b7 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-23T05:22:09Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-3d2f06fa395a474d886755ebd8d421b72022-12-21T17:58:40ZengBMCBMC Plant Biology1471-22292019-06-0119111610.1186/s12870-019-1824-9Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.)Sha Li0Zihan Liu1Yulin Jia2Jiali Ye3Xuetong Yang4Lingli Zhang5Xiyue Song6College of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityAbstract Background Thermo-sensitive male-sterility based on Aegilops kotschyi cytoplasm (K-TCMS) plays an important role in hybrid wheat breeding. This has important possible applications in two-line hybrid wheat breeding but the genetic basis and molecular regulation mechanism related to fertility restoration are poorly understood. In this study, comparative transcriptome profiling based on RNA sequencing was conducted for two near-isogenic lines comprising KTM3315R and its sterile counterpart KTM3315A, a total of six samples (3 repetitions per group), in order to identify fertility restoration genes and their metabolic pathways. Results In total, 2642 significant differentially expressed genes (DEGs) were detected, among which 1238 were down-regulated and 1404 were up-regulated in fertile anthers. Functional annotation enrichment analysis identified important pathways related to fertility restoration, such as carbohydrate metabolism, phenylpropanoid metabolism and biosynthesis, as well as candidate genes encoding pectin methylesterase and flavanone 3-hydroxylase. Moreover, transcription factor analysis showed that a large number of DEGs were mainly involved with the WRKY, bHLH, and MYB transcription factor families. Determination of total soluble sugar and flavonoid contents demonstrated that important metabolic pathways and candidate genes are associated with fertility restoration. Twelve DEGs were selected and detected by quantitative reverse-transcribed PCR, and the results indicated that the transcriptome sequencing results were reliable. Conclusions Our results indicate that identified DEGs were related to the fertility restoration and they proved to be crucial in Aegilops kotschyi cytoplasm. These findings also provide a basis for exploring the molecular regulation mechanism associated with wheat fertility restoration as well as screening and cloning related genes.http://link.springer.com/article/10.1186/s12870-019-1824-9Comparative transcriptomeCytoplasmic male sterilityFlavanone 3-hydroxylasePectin methylesteraseWheat (Triticum aestivum L.)WRKY transcription factor |
| spellingShingle | Sha Li Zihan Liu Yulin Jia Jiali Ye Xuetong Yang Lingli Zhang Xiyue Song Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.) BMC Plant Biology Comparative transcriptome Cytoplasmic male sterility Flavanone 3-hydroxylase Pectin methylesterase Wheat (Triticum aestivum L.) WRKY transcription factor |
| title | Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.) |
| title_full | Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.) |
| title_fullStr | Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.) |
| title_short | Analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with Aegilops kotschyi cytoplasm in wheat (Triticum aestivum L.) |
| title_sort | analysis of metabolic pathways related to fertility restoration and identification of fertility candidate genes associated with aegilops kotschyi cytoplasm in wheat triticum aestivum l |
| topic | Comparative transcriptome Cytoplasmic male sterility Flavanone 3-hydroxylase Pectin methylesterase Wheat (Triticum aestivum L.) WRKY transcription factor |
| url | http://link.springer.com/article/10.1186/s12870-019-1824-9 |
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