Age-Dependent Alterations in Platelet Mitochondrial Respiration
Mitochondrial dysfunction is an important cellular hallmark of aging and neurodegeneration. Platelets are a useful model to study the systemic manifestations of mitochondrial dysfunction. To evaluate the age dependence of mitochondrial parameters, citrate synthase activity, respiratory chain complex...
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MDPI AG
2023-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/6/1564 |
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| author | Zdeněk Fišar Jana Hroudová Martina Zvěřová Roman Jirák Jiří Raboch Eva Kitzlerová |
| author_facet | Zdeněk Fišar Jana Hroudová Martina Zvěřová Roman Jirák Jiří Raboch Eva Kitzlerová |
| author_sort | Zdeněk Fišar |
| collection | DOAJ |
| description | Mitochondrial dysfunction is an important cellular hallmark of aging and neurodegeneration. Platelets are a useful model to study the systemic manifestations of mitochondrial dysfunction. To evaluate the age dependence of mitochondrial parameters, citrate synthase activity, respiratory chain complex activity, and oxygen consumption kinetics were assessed. The effect of cognitive impairment was examined by comparing the age dependence of mitochondrial parameters in healthy individuals and those with neuropsychiatric disease. The study found a significant negative slope of age-dependence for both the activity of individual mitochondrial enzymes (citrate synthase and complex II) and parameters of mitochondrial respiration in intact platelets (routine respiration, maximum capacity of electron transport system, and respiratory rate after complex I inhibition). However, there was no significant difference in the age-related changes of mitochondrial parameters between individuals with and without cognitive impairment. These findings highlight the potential of measuring mitochondrial respiration in intact platelets as a means to assess age-related mitochondrial dysfunction. The results indicate that drugs and interventions targeting mitochondrial respiration may have the potential to slow down or eliminate certain aging and neurodegenerative processes. Mitochondrial respiration in platelets holds promise as a biomarker of aging, irrespective of the degree of cognitive impairment. |
| first_indexed | 2024-03-11T02:44:34Z |
| format | Article |
| id | doaj.art-3d2fb26ba6394849b0a29661dda1730f |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T02:44:34Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-3d2fb26ba6394849b0a29661dda1730f2023-11-18T09:25:07ZengMDPI AGBiomedicines2227-90592023-05-01116156410.3390/biomedicines11061564Age-Dependent Alterations in Platelet Mitochondrial RespirationZdeněk Fišar0Jana Hroudová1Martina Zvěřová2Roman Jirák3Jiří Raboch4Eva Kitzlerová5Department of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 120 00 Prague, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 120 00 Prague, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 120 00 Prague, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 120 00 Prague, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 120 00 Prague, Czech RepublicDepartment of Psychiatry, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 11, 120 00 Prague, Czech RepublicMitochondrial dysfunction is an important cellular hallmark of aging and neurodegeneration. Platelets are a useful model to study the systemic manifestations of mitochondrial dysfunction. To evaluate the age dependence of mitochondrial parameters, citrate synthase activity, respiratory chain complex activity, and oxygen consumption kinetics were assessed. The effect of cognitive impairment was examined by comparing the age dependence of mitochondrial parameters in healthy individuals and those with neuropsychiatric disease. The study found a significant negative slope of age-dependence for both the activity of individual mitochondrial enzymes (citrate synthase and complex II) and parameters of mitochondrial respiration in intact platelets (routine respiration, maximum capacity of electron transport system, and respiratory rate after complex I inhibition). However, there was no significant difference in the age-related changes of mitochondrial parameters between individuals with and without cognitive impairment. These findings highlight the potential of measuring mitochondrial respiration in intact platelets as a means to assess age-related mitochondrial dysfunction. The results indicate that drugs and interventions targeting mitochondrial respiration may have the potential to slow down or eliminate certain aging and neurodegenerative processes. Mitochondrial respiration in platelets holds promise as a biomarker of aging, irrespective of the degree of cognitive impairment.https://www.mdpi.com/2227-9059/11/6/1564agingplateletmitochondriarespiratory chain complexmitochondrial respirationcognitive decline |
| spellingShingle | Zdeněk Fišar Jana Hroudová Martina Zvěřová Roman Jirák Jiří Raboch Eva Kitzlerová Age-Dependent Alterations in Platelet Mitochondrial Respiration Biomedicines aging platelet mitochondria respiratory chain complex mitochondrial respiration cognitive decline |
| title | Age-Dependent Alterations in Platelet Mitochondrial Respiration |
| title_full | Age-Dependent Alterations in Platelet Mitochondrial Respiration |
| title_fullStr | Age-Dependent Alterations in Platelet Mitochondrial Respiration |
| title_full_unstemmed | Age-Dependent Alterations in Platelet Mitochondrial Respiration |
| title_short | Age-Dependent Alterations in Platelet Mitochondrial Respiration |
| title_sort | age dependent alterations in platelet mitochondrial respiration |
| topic | aging platelet mitochondria respiratory chain complex mitochondrial respiration cognitive decline |
| url | https://www.mdpi.com/2227-9059/11/6/1564 |
| work_keys_str_mv | AT zdenekfisar agedependentalterationsinplateletmitochondrialrespiration AT janahroudova agedependentalterationsinplateletmitochondrialrespiration AT martinazverova agedependentalterationsinplateletmitochondrialrespiration AT romanjirak agedependentalterationsinplateletmitochondrialrespiration AT jiriraboch agedependentalterationsinplateletmitochondrialrespiration AT evakitzlerova agedependentalterationsinplateletmitochondrialrespiration |