Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent
Chromium, a transition metal element widely distributed in the earth's crust, with the valence from −2 to +6, but exists mainly in the form of trivalent and hexavalent states. Hexavalent chromium Chromium (VI) is often found in soil and ground water due to its widespread industrial use such as...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Case Studies in Chemical and Environmental Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666016423001640 |
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| author | Ahmad Fatima Abdulmalik Hafeez Muhammad Yakasai Shehu Usman Jahun Bashir Muhammad Ahmad Hussaini Jagaba Salihu Ibrahim Abba Babandi Mohd Yunus Shukor |
| author_facet | Ahmad Fatima Abdulmalik Hafeez Muhammad Yakasai Shehu Usman Jahun Bashir Muhammad Ahmad Hussaini Jagaba Salihu Ibrahim Abba Babandi Mohd Yunus Shukor |
| author_sort | Ahmad Fatima Abdulmalik |
| collection | DOAJ |
| description | Chromium, a transition metal element widely distributed in the earth's crust, with the valence from −2 to +6, but exists mainly in the form of trivalent and hexavalent states. Hexavalent chromium Chromium (VI) is often found in soil and ground water due to its widespread industrial use such as tannery, electroplating, and steel industries. Chromium (VI) is toxic, mutagenic, carcinogenic, and teratogenic and much more toxic to many plants, animals, and bacteria inhabiting aquatic environments because it is motile, highly toxic, soluble in water and it is a strong oxidizing agent that causes severe damage to cell membranes while the trivalent chromium Cr (III) is the most stable form of chromium and relatively immobile in the aquatic system due to its lower solubility. This study therefore, aims at identifying a bacterium that can reduce Chromium (IV) to (III) using a pour plate technique on Luria Bertani medium amended with K2Cr2O7. Graphs and tables were used for the data analysis. The effects of incubation time, pH, temperature, inoculum size, and potassium dichromate (K2Cr2O7) concentration were then examined to characterize the bacterium based on one factor at a time. To assess Chromium (VI) reduction, a spectrophotometric study of the 1, 5-diphenyl carbazide test (DPC) at 370 nm was utilized. By using molecular phylogenetic analysis and partial 16s ribosomal RNA analysis, the isolate was found to be Acinetobacter sp. strain BUK_BCH_BTE 5. The findings demonstrated that tryptic soy broth (TSB), with an optimal K2Cr2O7 level of 200 mg/L, pH of 6.5, 35 °C temperature, inoculum size of 2%, as well as incubation time of 48 h, was the most successful approach for decreasing chromium (VI). Following Nickel (Ni), the isolate was found to withstand the highest doses of Lead (Pb), Mercury (Hg), Zink (Zn), Iron (Fe), Cadmium (Cd), Arsenic (As), and Cupper, in that order. A reduced culture's (supernatant) larvacidal bioassay showed a 30% decrease in toxicity in just 48 hours, indicating that the isolate is a promising candidate, and that the reduction method is less dangerous for decontaminating chromium-contaminated environments. |
| first_indexed | 2024-03-09T14:03:43Z |
| format | Article |
| id | doaj.art-3d318b1b578245489c9aad067a246e7d |
| institution | Directory Open Access Journal |
| issn | 2666-0164 |
| language | English |
| last_indexed | 2024-03-09T14:03:43Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Case Studies in Chemical and Environmental Engineering |
| spelling | doaj.art-3d318b1b578245489c9aad067a246e7d2023-11-30T05:11:06ZengElsevierCase Studies in Chemical and Environmental Engineering2666-01642023-12-018100459Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluentAhmad Fatima Abdulmalik0Hafeez Muhammad Yakasai1Shehu Usman2Jahun Bashir Muhammad3Ahmad Hussaini Jagaba4Salihu Ibrahim5Abba Babandi6Mohd Yunus Shukor7Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, Nigeria; Skyline University Nigeria, No. 2 Zaria Road, Kano State, Nigeria; Corresponding author. Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, Nigeria.Department of Plant Science and Biotechnology, Federal University, Dutsin-Ma, Katsina, NigeriaDepartment of Biology, Federal Collage of Education Technical, Bichi, Kano, NigeriaDepartment of Civil Engineering, Abubakar Tafawa Balewa University, Bauchi, Nigeria; Corresponding author. Department of Civil Engineering, Abubakar Tafawa Balewa University, Bauchi, Nigeria.Centre for Biotechnology Research, Bayero University Kano, P.M.B 3011, Kano State, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, MalaysiaChromium, a transition metal element widely distributed in the earth's crust, with the valence from −2 to +6, but exists mainly in the form of trivalent and hexavalent states. Hexavalent chromium Chromium (VI) is often found in soil and ground water due to its widespread industrial use such as tannery, electroplating, and steel industries. Chromium (VI) is toxic, mutagenic, carcinogenic, and teratogenic and much more toxic to many plants, animals, and bacteria inhabiting aquatic environments because it is motile, highly toxic, soluble in water and it is a strong oxidizing agent that causes severe damage to cell membranes while the trivalent chromium Cr (III) is the most stable form of chromium and relatively immobile in the aquatic system due to its lower solubility. This study therefore, aims at identifying a bacterium that can reduce Chromium (IV) to (III) using a pour plate technique on Luria Bertani medium amended with K2Cr2O7. Graphs and tables were used for the data analysis. The effects of incubation time, pH, temperature, inoculum size, and potassium dichromate (K2Cr2O7) concentration were then examined to characterize the bacterium based on one factor at a time. To assess Chromium (VI) reduction, a spectrophotometric study of the 1, 5-diphenyl carbazide test (DPC) at 370 nm was utilized. By using molecular phylogenetic analysis and partial 16s ribosomal RNA analysis, the isolate was found to be Acinetobacter sp. strain BUK_BCH_BTE 5. The findings demonstrated that tryptic soy broth (TSB), with an optimal K2Cr2O7 level of 200 mg/L, pH of 6.5, 35 °C temperature, inoculum size of 2%, as well as incubation time of 48 h, was the most successful approach for decreasing chromium (VI). Following Nickel (Ni), the isolate was found to withstand the highest doses of Lead (Pb), Mercury (Hg), Zink (Zn), Iron (Fe), Cadmium (Cd), Arsenic (As), and Cupper, in that order. A reduced culture's (supernatant) larvacidal bioassay showed a 30% decrease in toxicity in just 48 hours, indicating that the isolate is a promising candidate, and that the reduction method is less dangerous for decontaminating chromium-contaminated environments.http://www.sciencedirect.com/science/article/pii/S2666016423001640AcinetobacterBioremediationChromate-reducing bacteriaHexavalent chromiumTannery effluentToxicity |
| spellingShingle | Ahmad Fatima Abdulmalik Hafeez Muhammad Yakasai Shehu Usman Jahun Bashir Muhammad Ahmad Hussaini Jagaba Salihu Ibrahim Abba Babandi Mohd Yunus Shukor Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent Case Studies in Chemical and Environmental Engineering Acinetobacter Bioremediation Chromate-reducing bacteria Hexavalent chromium Tannery effluent Toxicity |
| title | Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent |
| title_full | Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent |
| title_fullStr | Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent |
| title_full_unstemmed | Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent |
| title_short | Characterization and invitro toxicity assay of bio-reduced hexavalent chromium by Acinetobacter sp. isolated from tannery effluent |
| title_sort | characterization and invitro toxicity assay of bio reduced hexavalent chromium by acinetobacter sp isolated from tannery effluent |
| topic | Acinetobacter Bioremediation Chromate-reducing bacteria Hexavalent chromium Tannery effluent Toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2666016423001640 |
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