Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress
Abstract Background Integrins are closely related to mechanical conduction and play a crucial role in the osteogenesis of human mesenchymal stem cells. Here we wondered whether tensile stress could influence cell differentiation through integrin αVβ3. Methods We inhibited the function of integrin αV...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Cell Communication and Signaling |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12964-022-01027-7 |
| _version_ | 1797636744060862464 |
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| author | Yan Peng Rongmei Qu Yuchao Yang Tingyu Fan Bing Sun Asmat Ullah Khan Shutong Wu Wenqing Liu Jinhui Zhu Junxin Chen Xiaoqing Li Jingxing Dai Jun Ouyang |
| author_facet | Yan Peng Rongmei Qu Yuchao Yang Tingyu Fan Bing Sun Asmat Ullah Khan Shutong Wu Wenqing Liu Jinhui Zhu Junxin Chen Xiaoqing Li Jingxing Dai Jun Ouyang |
| author_sort | Yan Peng |
| collection | DOAJ |
| description | Abstract Background Integrins are closely related to mechanical conduction and play a crucial role in the osteogenesis of human mesenchymal stem cells. Here we wondered whether tensile stress could influence cell differentiation through integrin αVβ3. Methods We inhibited the function of integrin αVβ3 of human mesenchymal stem cells by treating with c(RGDyk). Using cytochalasin D and verteporfin to inhibit polymerization of microfilament and function of nuclear Yes-associated protein (YAP), respectively. For each application, mesenchymal stem cells were loaded by cyclic tensile stress of 10% at 0.5 Hz for 2 h daily. Mesenchymal stem cells were harvested on day 7 post-treatment. Western blotting and quantitative RT-PCR were used to detect the expression of alkaline phosphatase (ALP), RUNX2, β-actin, integrin αVβ3, talin-1, vinculin, FAK, and nuclear YAP. Immunofluorescence staining detected vinculin, actin filaments, and YAP nuclear localization. Results Cyclic tensile stress could increase the expression of ALP and RUNX2. Inhibition of integrin αVβ3 activation led to rearrangement of actin filaments and downregulated the expression of ALP, RUNX2 and promoted YAP nuclear localization. When microfilament polymerization was inhibited, ALP, RUNX2, and nuclear YAP nuclear localization decreased. Inhibition of YAP nuclear localization could reduce the expression of ALP and RUNX2. Conclusions Cyclic tensile stress promotes early osteogenesis of human mesenchymal stem cells via the integrin αVβ3-actin filaments axis. YAP nuclear localization participates in this process of human mesenchymal stem cells. Video Abstract |
| first_indexed | 2024-03-11T12:39:29Z |
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| id | doaj.art-3d33dd5f17dd4ee99bcbc40f1b3f030b |
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| issn | 1478-811X |
| language | English |
| last_indexed | 2024-03-11T12:39:29Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-3d33dd5f17dd4ee99bcbc40f1b3f030b2023-11-05T12:25:10ZengBMCCell Communication and Signaling1478-811X2023-10-0121111110.1186/s12964-022-01027-7Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stressYan Peng0Rongmei Qu1Yuchao Yang2Tingyu Fan3Bing Sun4Asmat Ullah Khan5Shutong Wu6Wenqing Liu7Jinhui Zhu8Junxin Chen9Xiaoqing Li10Jingxing Dai11Jun Ouyang12Guangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityShenzhen Andy New Material Technology Co., LTDShenzhen Andy New Material Technology Co., LTDGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityGuangdong Provincial Key Laboratory of Medical Biomechanics and Guangdong Engineering Research Center for Translation of Medical 3D Printing Application and National Virtual and Reality Experimental Education Center for Medical Morphology and National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical UniversityAbstract Background Integrins are closely related to mechanical conduction and play a crucial role in the osteogenesis of human mesenchymal stem cells. Here we wondered whether tensile stress could influence cell differentiation through integrin αVβ3. Methods We inhibited the function of integrin αVβ3 of human mesenchymal stem cells by treating with c(RGDyk). Using cytochalasin D and verteporfin to inhibit polymerization of microfilament and function of nuclear Yes-associated protein (YAP), respectively. For each application, mesenchymal stem cells were loaded by cyclic tensile stress of 10% at 0.5 Hz for 2 h daily. Mesenchymal stem cells were harvested on day 7 post-treatment. Western blotting and quantitative RT-PCR were used to detect the expression of alkaline phosphatase (ALP), RUNX2, β-actin, integrin αVβ3, talin-1, vinculin, FAK, and nuclear YAP. Immunofluorescence staining detected vinculin, actin filaments, and YAP nuclear localization. Results Cyclic tensile stress could increase the expression of ALP and RUNX2. Inhibition of integrin αVβ3 activation led to rearrangement of actin filaments and downregulated the expression of ALP, RUNX2 and promoted YAP nuclear localization. When microfilament polymerization was inhibited, ALP, RUNX2, and nuclear YAP nuclear localization decreased. Inhibition of YAP nuclear localization could reduce the expression of ALP and RUNX2. Conclusions Cyclic tensile stress promotes early osteogenesis of human mesenchymal stem cells via the integrin αVβ3-actin filaments axis. YAP nuclear localization participates in this process of human mesenchymal stem cells. Video Abstracthttps://doi.org/10.1186/s12964-022-01027-7OsteogenesisIntegrin αVβ3Tensile stressMesenchymal stem cellsYes-associated protein (YAP) |
| spellingShingle | Yan Peng Rongmei Qu Yuchao Yang Tingyu Fan Bing Sun Asmat Ullah Khan Shutong Wu Wenqing Liu Jinhui Zhu Junxin Chen Xiaoqing Li Jingxing Dai Jun Ouyang Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress Cell Communication and Signaling Osteogenesis Integrin αVβ3 Tensile stress Mesenchymal stem cells Yes-associated protein (YAP) |
| title | Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress |
| title_full | Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress |
| title_fullStr | Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress |
| title_full_unstemmed | Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress |
| title_short | Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress |
| title_sort | regulation of the integrin αvβ3 actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress |
| topic | Osteogenesis Integrin αVβ3 Tensile stress Mesenchymal stem cells Yes-associated protein (YAP) |
| url | https://doi.org/10.1186/s12964-022-01027-7 |
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