Anti-diabetic compounds from Uvaria dulcis Dunal
Medicinal plants have long been a source of lead compounds for drug discovery. Among these, the Annonaceae family has gained recognition for its potential to yield novel compounds, particularly those that can be used in the development of drugs targeting chronic diseases like diabetes mellitus (DM)....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024029931 |
| _version_ | 1797259776387710976 |
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| author | Passakorn Teerapongpisan Rachanida Praparatana Benjaporn Noppradit Surat Laphookhieo Panupong Puttarak |
| author_facet | Passakorn Teerapongpisan Rachanida Praparatana Benjaporn Noppradit Surat Laphookhieo Panupong Puttarak |
| author_sort | Passakorn Teerapongpisan |
| collection | DOAJ |
| description | Medicinal plants have long been a source of lead compounds for drug discovery. Among these, the Annonaceae family has gained recognition for its potential to yield novel compounds, particularly those that can be used in the development of drugs targeting chronic diseases like diabetes mellitus (DM). We employed various chromatographic methods to isolate bioactive compounds from the roots, leaves, and twigs of Uvaria dulcis Dunal. We used spectroscopic methods to determine the chemical structures of these compounds. We successfully identified twelve known compounds from various parts of U. dulcis: patchoulenon, polygochalcone, 2′3′-dihydroxy-4′,6′-dimethoxydihydrochalcone, 2′,3′-dihydroxy-4′,6′-dimethoxychalcone, chrysin, techochrysin, 8-hydroxy-5,7-dimethoxyflavanone, pinocembrin, 3-farnesylindole, onysilin, cinchonain la, and cinchonain lb. Interestingly, cinchonain la and cinchonain lb exhibited more potent anti-α-glucosidase activity than acarbose (standard drug), with IC50 values of 11.88 ± 1.41 μg/mL and 15.18 ± 1.19 μg/mL, respectively. Cinchonain la inhibited the DPP-IV enzyme, with IC50 value lower than the standard compound (diprotin A) at 81.78 ± 1.42 μg/mL. While 2′,3′-dihydroxy-4′,6′-dimethoxychalcone show more potent inhibitory effect than standard drug with IC50 value of 8.62 ± 1.19 μg/mL. Additionally, at a concentration of 10 μg/mL, cinchonain lb and 2′,3′-dihydroxy-4′,6′-dimethoxychalcone promoted glucose uptake in L6 myotubes cells to the same extent as 100 nM insulin. These findings suggest that cinchonain la, cinchonain lb, and 2′,3′-dihydroxy-4′,6′-dimethoxychalcone are the U. dulcis-derived bioactive compounds that hold promise as potential structures to use in the development of anti-diabetic drugs. |
| first_indexed | 2024-03-07T16:52:55Z |
| format | Article |
| id | doaj.art-3d34d1a505db4693852d8aa9490e61e4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:14:48Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3d34d1a505db4693852d8aa9490e61e42024-03-17T07:56:54ZengElsevierHeliyon2405-84402024-03-01105e26962Anti-diabetic compounds from Uvaria dulcis DunalPassakorn Teerapongpisan0Rachanida Praparatana1Benjaporn Noppradit2Surat Laphookhieo3Panupong Puttarak4Center of Chemical Innovation for Sustainability (CIS) and School of Science, Mae Fah Luang University, Chiang Rai 57100, ThailandFaculty of Medical Technology, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, ThailandCenter of Chemical Innovation for Sustainability (CIS) and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand; Medicinal Plant Innovation Center of Mae Fah Luang University, Chiang Rai 57100, Thailand; Corresponding author. Center of Chemical Innovation for Sustainability (CIS) and School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand; Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand; Corresponding author. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.Medicinal plants have long been a source of lead compounds for drug discovery. Among these, the Annonaceae family has gained recognition for its potential to yield novel compounds, particularly those that can be used in the development of drugs targeting chronic diseases like diabetes mellitus (DM). We employed various chromatographic methods to isolate bioactive compounds from the roots, leaves, and twigs of Uvaria dulcis Dunal. We used spectroscopic methods to determine the chemical structures of these compounds. We successfully identified twelve known compounds from various parts of U. dulcis: patchoulenon, polygochalcone, 2′3′-dihydroxy-4′,6′-dimethoxydihydrochalcone, 2′,3′-dihydroxy-4′,6′-dimethoxychalcone, chrysin, techochrysin, 8-hydroxy-5,7-dimethoxyflavanone, pinocembrin, 3-farnesylindole, onysilin, cinchonain la, and cinchonain lb. Interestingly, cinchonain la and cinchonain lb exhibited more potent anti-α-glucosidase activity than acarbose (standard drug), with IC50 values of 11.88 ± 1.41 μg/mL and 15.18 ± 1.19 μg/mL, respectively. Cinchonain la inhibited the DPP-IV enzyme, with IC50 value lower than the standard compound (diprotin A) at 81.78 ± 1.42 μg/mL. While 2′,3′-dihydroxy-4′,6′-dimethoxychalcone show more potent inhibitory effect than standard drug with IC50 value of 8.62 ± 1.19 μg/mL. Additionally, at a concentration of 10 μg/mL, cinchonain lb and 2′,3′-dihydroxy-4′,6′-dimethoxychalcone promoted glucose uptake in L6 myotubes cells to the same extent as 100 nM insulin. These findings suggest that cinchonain la, cinchonain lb, and 2′,3′-dihydroxy-4′,6′-dimethoxychalcone are the U. dulcis-derived bioactive compounds that hold promise as potential structures to use in the development of anti-diabetic drugs.http://www.sciencedirect.com/science/article/pii/S2405844024029931DiabetesUvaria dulcis Dunalα-GlucosidaseGlucose uptakeDipeptidyl peptidase-IV |
| spellingShingle | Passakorn Teerapongpisan Rachanida Praparatana Benjaporn Noppradit Surat Laphookhieo Panupong Puttarak Anti-diabetic compounds from Uvaria dulcis Dunal Heliyon Diabetes Uvaria dulcis Dunal α-Glucosidase Glucose uptake Dipeptidyl peptidase-IV |
| title | Anti-diabetic compounds from Uvaria dulcis Dunal |
| title_full | Anti-diabetic compounds from Uvaria dulcis Dunal |
| title_fullStr | Anti-diabetic compounds from Uvaria dulcis Dunal |
| title_full_unstemmed | Anti-diabetic compounds from Uvaria dulcis Dunal |
| title_short | Anti-diabetic compounds from Uvaria dulcis Dunal |
| title_sort | anti diabetic compounds from uvaria dulcis dunal |
| topic | Diabetes Uvaria dulcis Dunal α-Glucosidase Glucose uptake Dipeptidyl peptidase-IV |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024029931 |
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