Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice
Abstract Increased gut permeability is implicated in the initiation and extent of the cytokine inflammatory response associated with exertional heat stroke (EHS). The primary objective of this study was to determine if a five amino acid oral rehydration solution (5AAS), specifically designed for the...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-05-01
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| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.15681 |
| _version_ | 1797397468461137920 |
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| author | Michelle A. King Astrid Grosche Shauna M. Ward Jermaine A. Ward Anusree Sasidharan Thomas A. Mayer Mark L. Plamper Xiaodong Xu Matthew D. Ward Thomas L. Clanton Sadasivan Vidyasagar |
| author_facet | Michelle A. King Astrid Grosche Shauna M. Ward Jermaine A. Ward Anusree Sasidharan Thomas A. Mayer Mark L. Plamper Xiaodong Xu Matthew D. Ward Thomas L. Clanton Sadasivan Vidyasagar |
| author_sort | Michelle A. King |
| collection | DOAJ |
| description | Abstract Increased gut permeability is implicated in the initiation and extent of the cytokine inflammatory response associated with exertional heat stroke (EHS). The primary objective of this study was to determine if a five amino acid oral rehydration solution (5AAS), specifically designed for the protection of the gastrointestinal lining, would prolong time to EHS, maintain gut function and dampen the systemic inflammatory response (SIR) measured during EHS recovery. Male C57/BL6J mice instrumented with radiotelemetry were gavaged with 150 μL of 5AAS or H2O, and ≈12 h later were either exposed to an EHS protocol where mice exercised in a 37.5°C environmental chamber to a self‐limiting maximum core temperature (Tc,max) or performed the exercise control (EXC) protocol (25°C). 5AAS pretreatment attenuated hypothermia depth and length (p < 0.005), which are indicators of EHS severity during recovery, without any effect on physical performance or thermoregulatory responses in the heat as determined by percent body weight lost (≈9%), max speed (≈6 m/min), distance (≈700 m), time to Tc,max (≈160 min), thermal area (≈550°C∙min), and Tc,max (42.2°C). EHS groups treated with 5AAS showed a significant decrease in gut transepithelial conductance, decreased paracellular permeability, increased villus height, increased electrolyte absorption and changes in tight junction protein expression pattern suggestive of improved barrier integrity (p < 0.05). No differences were witnessed between EHS groups in acute phase response markers of liver, circulating SIR markers, or indicators of organ damage during recovery. These results suggest that a 5AAS improves Tc regulation during EHS recovery through maintaining mucosal function and integrity. |
| first_indexed | 2024-03-09T01:10:27Z |
| format | Article |
| id | doaj.art-3d38e6261ba14746adbd931dad9aa400 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:10:27Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-3d38e6261ba14746adbd931dad9aa4002023-12-11T05:30:53ZengWileyPhysiological Reports2051-817X2023-05-011110n/an/a10.14814/phy2.15681Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male miceMichelle A. King0Astrid Grosche1Shauna M. Ward2Jermaine A. Ward3Anusree Sasidharan4Thomas A. Mayer5Mark L. Plamper6Xiaodong Xu7Matthew D. Ward8Thomas L. Clanton9Sadasivan Vidyasagar10Thermal and Mountain Medicine Division United States Army Research Institute of Environmental Medicine Natick Massachusetts USARadiation Oncology University of Florida College of Medicine Gainesville Florida USAThermal and Mountain Medicine Division United States Army Research Institute of Environmental Medicine Natick Massachusetts USAThermal and Mountain Medicine Division United States Army Research Institute of Environmental Medicine Natick Massachusetts USARadiation Oncology University of Florida College of Medicine Gainesville Florida USAThermal and Mountain Medicine Division United States Army Research Institute of Environmental Medicine Natick Massachusetts USAThermal and Mountain Medicine Division United States Army Research Institute of Environmental Medicine Natick Massachusetts USARadiation Oncology University of Florida College of Medicine Gainesville Florida USAThermal and Mountain Medicine Division United States Army Research Institute of Environmental Medicine Natick Massachusetts USAHealth and Human Performance University of Florida Gainesville Florida USARadiation Oncology University of Florida College of Medicine Gainesville Florida USAAbstract Increased gut permeability is implicated in the initiation and extent of the cytokine inflammatory response associated with exertional heat stroke (EHS). The primary objective of this study was to determine if a five amino acid oral rehydration solution (5AAS), specifically designed for the protection of the gastrointestinal lining, would prolong time to EHS, maintain gut function and dampen the systemic inflammatory response (SIR) measured during EHS recovery. Male C57/BL6J mice instrumented with radiotelemetry were gavaged with 150 μL of 5AAS or H2O, and ≈12 h later were either exposed to an EHS protocol where mice exercised in a 37.5°C environmental chamber to a self‐limiting maximum core temperature (Tc,max) or performed the exercise control (EXC) protocol (25°C). 5AAS pretreatment attenuated hypothermia depth and length (p < 0.005), which are indicators of EHS severity during recovery, without any effect on physical performance or thermoregulatory responses in the heat as determined by percent body weight lost (≈9%), max speed (≈6 m/min), distance (≈700 m), time to Tc,max (≈160 min), thermal area (≈550°C∙min), and Tc,max (42.2°C). EHS groups treated with 5AAS showed a significant decrease in gut transepithelial conductance, decreased paracellular permeability, increased villus height, increased electrolyte absorption and changes in tight junction protein expression pattern suggestive of improved barrier integrity (p < 0.05). No differences were witnessed between EHS groups in acute phase response markers of liver, circulating SIR markers, or indicators of organ damage during recovery. These results suggest that a 5AAS improves Tc regulation during EHS recovery through maintaining mucosal function and integrity.https://doi.org/10.14814/phy2.15681cytokinesexercisegastrointestinalinflammationpermeabilitysupplementation |
| spellingShingle | Michelle A. King Astrid Grosche Shauna M. Ward Jermaine A. Ward Anusree Sasidharan Thomas A. Mayer Mark L. Plamper Xiaodong Xu Matthew D. Ward Thomas L. Clanton Sadasivan Vidyasagar Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice Physiological Reports cytokines exercise gastrointestinal inflammation permeability supplementation |
| title | Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice |
| title_full | Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice |
| title_fullStr | Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice |
| title_full_unstemmed | Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice |
| title_short | Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice |
| title_sort | amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male mice |
| topic | cytokines exercise gastrointestinal inflammation permeability supplementation |
| url | https://doi.org/10.14814/phy2.15681 |
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