Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes
Avena sativa (AS) is a well-known food crop and an important medicinal plant. It has been used for the treatment of various diseases, particularly for the treatment of inflammatory and cardiovascular diseases. However, the scientific rationale and mechanisms by which it functions in these diseases i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Advance Educational Institute & Research Centre
2013-12-01
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| Series: | International Journal of Endorsing Health Science Research |
| Subjects: | |
| Online Access: | http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/35/291 |
| _version_ | 1818909106367365120 |
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| author | Sagheer Ahmed Saima Gul Humera Gul Muhammad Hanif Bangash |
| author_facet | Sagheer Ahmed Saima Gul Humera Gul Muhammad Hanif Bangash |
| author_sort | Sagheer Ahmed |
| collection | DOAJ |
| description | Avena sativa (AS) is a well-known food crop and an important medicinal plant. It has been used for the treatment of various diseases, particularly for the treatment of inflammatory and cardiovascular diseases. However, the scientific rationale and mechanisms by which it functions in these diseases is still un- known. This study was designed to explore the inhibitory activity of AS aqueous, n-hexane and butanolic fractions on arachidonic acid (AA) metabolism. For this purpose these fractions of AS were screened for the presence of activities against AA metabolites and their effectiveness was further evaluated by studying platelet aggregation induced by AA, adenosine diphosphate (ADP), platelet activating factor (PAF), and collageAA metabolism was studied by thin layer chromatography system while platelet aggregation was measured by dual channel Lumiaggregometer. Aqueous and n-hexane fractions of AS were totally ineffective against AA metabolism and platelet aggregation. However, butanolic fraction inhibited the AA metabolites- thromboxane B2 (TXB2) through cyclooxygenase (COX) pathway and lipoxygenase product-1 (LP-1) and 12-hydroxyeicosatetraenoic acids (12-HETE) through lipoxygenase (LOX) pathway. Similarly butanolic fraction of AS showed strong inhibition against AA, PAF-induced aggregation but was less potent against ADP.AS possesses components which can inhibit AA metabolism and platelet aggregation. This may be one of the underlying mechanisms of their actions in cardiovascular and inflammatory diseases. |
| first_indexed | 2024-12-19T22:21:38Z |
| format | Article |
| id | doaj.art-3d398e79577d4434a4c43742db2090df |
| institution | Directory Open Access Journal |
| issn | 2307-3748 2310-3841 |
| language | English |
| last_indexed | 2024-12-19T22:21:38Z |
| publishDate | 2013-12-01 |
| publisher | Advance Educational Institute & Research Centre |
| record_format | Article |
| series | International Journal of Endorsing Health Science Research |
| spelling | doaj.art-3d398e79577d4434a4c43742db2090df2022-12-21T20:03:37ZengAdvance Educational Institute & Research CentreInternational Journal of Endorsing Health Science Research2307-37482310-38412013-12-01126265https://doi.org/10.29052/IJEHSR.v1.i2.2013.62-65Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymesSagheer Ahmed0Saima Gul1Humera Gul2Muhammad Hanif Bangash3PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Department of Pharmacy, Kohat University of Science & Technology, Kohat, PakistanDepartment of Zoology, Abdul Wali Khan University, Mardan, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan, PakistanChashma Nuclear Power Plant, Chashma city, PakistanAvena sativa (AS) is a well-known food crop and an important medicinal plant. It has been used for the treatment of various diseases, particularly for the treatment of inflammatory and cardiovascular diseases. However, the scientific rationale and mechanisms by which it functions in these diseases is still un- known. This study was designed to explore the inhibitory activity of AS aqueous, n-hexane and butanolic fractions on arachidonic acid (AA) metabolism. For this purpose these fractions of AS were screened for the presence of activities against AA metabolites and their effectiveness was further evaluated by studying platelet aggregation induced by AA, adenosine diphosphate (ADP), platelet activating factor (PAF), and collageAA metabolism was studied by thin layer chromatography system while platelet aggregation was measured by dual channel Lumiaggregometer. Aqueous and n-hexane fractions of AS were totally ineffective against AA metabolism and platelet aggregation. However, butanolic fraction inhibited the AA metabolites- thromboxane B2 (TXB2) through cyclooxygenase (COX) pathway and lipoxygenase product-1 (LP-1) and 12-hydroxyeicosatetraenoic acids (12-HETE) through lipoxygenase (LOX) pathway. Similarly butanolic fraction of AS showed strong inhibition against AA, PAF-induced aggregation but was less potent against ADP.AS possesses components which can inhibit AA metabolism and platelet aggregation. This may be one of the underlying mechanisms of their actions in cardiovascular and inflammatory diseases.http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/35/291asbronchodilatorcyclooxygenaselipoxygenaseaqueous fractionbutanolic fraction. |
| spellingShingle | Sagheer Ahmed Saima Gul Humera Gul Muhammad Hanif Bangash Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes International Journal of Endorsing Health Science Research as bronchodilator cyclooxygenase lipoxygenase aqueous fraction butanolic fraction. |
| title | Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes |
| title_full | Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes |
| title_fullStr | Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes |
| title_full_unstemmed | Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes |
| title_short | Anti-inflammatory and anti-platelet activities of Avena Sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes |
| title_sort | anti inflammatory and anti platelet activities of avena sativa are mediated through the inhibition of cyclooxygenase and lipoxygenase enzymes |
| topic | as bronchodilator cyclooxygenase lipoxygenase aqueous fraction butanolic fraction. |
| url | http://aeirc-edu.com/ojs14/index.php/IJEHSR/article/view/35/291 |
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