Disclosing bias in bisulfite assay: MethPrimers underestimate high DNA methylation.

Disclosing bias in bisulfite assay: MethPrimers underestimate high DNA methylation.

Discordant results obtained in bisulfite assays using MethPrimers (PCR primers designed using MethPrimer software or assuming that non-CpGs cytosines are non methylated) versus primers insensitive to cytosine methylation lead us to hypothesize a technical bias. We therefore used the two kinds of pri...

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Bibliographic Details
Main Authors: Andrea Fuso, Giampiero Ferraguti, Sigfrido Scarpa, Isidre Ferrer, Marco Lucarelli
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4333220?pdf=render

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