Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa
Genotoxic evaluation is an important step for a product that is aimed for human consumption. A beverage composed of pseudocereals with highly nutritious elements like quinua (Chenopodium quinoa Willd.), kiwicha (Amaranthus caudatus L.) and kañiwa (Chenopodium pallidicaule Aellen) was prepared to red...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Nacional Mayor de San Marcos
2014-12-01
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| Series: | Revista Peruana de Biología |
| Subjects: | |
| Online Access: | http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/10899 |
| _version_ | 1818007570201182208 |
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| author | Francia D.P. Huaman Emily M. Toscano Oscar Acosta Diana E. Rojas Miguel A. Inocente Diana P. Garrido María L. Guevara-Fujita |
| author_facet | Francia D.P. Huaman Emily M. Toscano Oscar Acosta Diana E. Rojas Miguel A. Inocente Diana P. Garrido María L. Guevara-Fujita |
| author_sort | Francia D.P. Huaman |
| collection | DOAJ |
| description | Genotoxic evaluation is an important step for a product that is aimed for human consumption. A beverage composed of pseudocereals with highly nutritious elements like quinua (Chenopodium quinoa Willd.), kiwicha (Amaranthus caudatus L.) and kañiwa (Chenopodium pallidicaule Aellen) was prepared to reduce lipid contents in a group of volunteers. The objective of the present study is to evaluate the genotoxic potential of an experimental beverage using two in vitro tests that have been validated by international agencies. For the Ames test, two strains of Salmonella typhimurium (TA98 and TA100) with and without microsomal fraction (S9) were used. Four doses of the beverage were tested and also a possible protective effect (same four doses of beverage added to plates with mutagens). Cultures of binucleated lymphocytes and five doses of the beverage were used for the micronucleus test. Both Ames and the micronucleus tests showed the beverage has not genotoxic effect in all tested doses. However, in evaluating the possible protective effect of the beverage, it would be evident that on the contrary, the mutagenic effect of mutagens used for each strain is enhanced. These results suggest that additional tests should be performed to check the genotoxic potential of this beverage before consumption. |
| first_indexed | 2024-04-14T05:17:24Z |
| format | Article |
| id | doaj.art-3d3e07777ca74b0ab858acb0704a6cbc |
| institution | Directory Open Access Journal |
| issn | 1561-0837 1727-9933 |
| language | English |
| last_indexed | 2024-04-14T05:17:24Z |
| publishDate | 2014-12-01 |
| publisher | Universidad Nacional Mayor de San Marcos |
| record_format | Article |
| series | Revista Peruana de Biología |
| spelling | doaj.art-3d3e07777ca74b0ab858acb0704a6cbc2022-12-22T02:10:19ZengUniversidad Nacional Mayor de San MarcosRevista Peruana de Biología1561-08371727-99332014-12-0121325125810.15381/rpb.v21i3.108999722Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwaFrancia D.P. Huaman0Emily M. Toscano1Oscar Acosta2Diana E. Rojas3Miguel A. Inocente4Diana P. Garrido5María L. Guevara-Fujita6(1) Centro de Genética y Biología Molecular (CGBM), Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.(2) Centro de Medicina Tradicional (CMT). Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.(1) Centro de Genética y Biología Molecular (CGBM), Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.(1) Centro de Genética y Biología Molecular (CGBM), Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.(2) Centro de Medicina Tradicional (CMT). Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.(2) Centro de Medicina Tradicional (CMT). Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.(1) Centro de Genética y Biología Molecular (CGBM), Instituto de Investigación de la Facultad de Medicina Humana de la Universidad San Martín de Porres. Av. Alameda del Corregidor N°1531. Urb. Los Sirius III Etapa. La Molina, Lima 12, Perú.Genotoxic evaluation is an important step for a product that is aimed for human consumption. A beverage composed of pseudocereals with highly nutritious elements like quinua (Chenopodium quinoa Willd.), kiwicha (Amaranthus caudatus L.) and kañiwa (Chenopodium pallidicaule Aellen) was prepared to reduce lipid contents in a group of volunteers. The objective of the present study is to evaluate the genotoxic potential of an experimental beverage using two in vitro tests that have been validated by international agencies. For the Ames test, two strains of Salmonella typhimurium (TA98 and TA100) with and without microsomal fraction (S9) were used. Four doses of the beverage were tested and also a possible protective effect (same four doses of beverage added to plates with mutagens). Cultures of binucleated lymphocytes and five doses of the beverage were used for the micronucleus test. Both Ames and the micronucleus tests showed the beverage has not genotoxic effect in all tested doses. However, in evaluating the possible protective effect of the beverage, it would be evident that on the contrary, the mutagenic effect of mutagens used for each strain is enhanced. These results suggest that additional tests should be performed to check the genotoxic potential of this beverage before consumption.http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/10899QuinuaKiwichaKañiwaPrueba de AmesPrueba de Micronúcleos. |
| spellingShingle | Francia D.P. Huaman Emily M. Toscano Oscar Acosta Diana E. Rojas Miguel A. Inocente Diana P. Garrido María L. Guevara-Fujita Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa Revista Peruana de Biología Quinua Kiwicha Kañiwa Prueba de Ames Prueba de Micronúcleos. |
| title | Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa |
| title_full | Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa |
| title_fullStr | Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa |
| title_full_unstemmed | Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa |
| title_short | Genotoxicity study of an experimental beverage made with quinua, kiwicha and kañiwa |
| title_sort | genotoxicity study of an experimental beverage made with quinua kiwicha and kaniwa |
| topic | Quinua Kiwicha Kañiwa Prueba de Ames Prueba de Micronúcleos. |
| url | http://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/10899 |
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