Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice
Since the epidemic in 2007, studies on vector competence for Zika virus (ZIKV) have intensified, showing that the transmission efficiency varies depending on the vector population, ZIKV strain, and dose of the infectious blood meal. In this study, we aimed to investigate the replication of African a...
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2021-06-01
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author | Tey Putita Ou Heidi Auerswald Saraden In Borin Peng Senglong Pang Sébastien Boyer Rithy Choeung Myrielle Dupont-Rouzeyrol Philippe Dussart Veasna Duong |
author_facet | Tey Putita Ou Heidi Auerswald Saraden In Borin Peng Senglong Pang Sébastien Boyer Rithy Choeung Myrielle Dupont-Rouzeyrol Philippe Dussart Veasna Duong |
author_sort | Tey Putita Ou |
collection | DOAJ |
description | Since the epidemic in 2007, studies on vector competence for Zika virus (ZIKV) have intensified, showing that the transmission efficiency varies depending on the vector population, ZIKV strain, and dose of the infectious blood meal. In this study, we aimed to investigate the replication of African and Asian ZIKV strains in vitro and in vivo in order to reveal their phenotypic differences. In addition, we investigated the vector competence of Cambodian <i>Aedes aegypti</i> (<i>Ae. aegypti</i>) mosquitoes (urban and rural) for these ZIKV strains. We observed a significantly higher pathogenicity of the African ZIKV strain in vitro (in mosquito and mammalian cells), and in vivo in both <i>Ae. aegypti</i> and mice. Both mosquito populations were competent to transmit ZIKV as early as 7 days p.i., depending on the population and the ZIKV strain. <i>Ae. aegypti</i> from rural habitats showed significant higher transmission and survival rates than those from urban. We observed the highest transmission efficiency for the African ZIKV isolate (93.3% 14 days p.i.) and for the Cambodian ZIKV isolate (80% 14 days p.i.). Overall, our results highlight the phenotypic differences of the ZIKV lineages and the potential risk of ZIKV transmission by <i>Ae. aegypti</i> mosquitoes. Further investigations of Cambodian mosquito species and ZIKV specific surveillance in humans is necessary in order to improve the local risk assessment. |
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spelling | doaj.art-3d3e8f31ed8d4c949b903b4af206a5442023-11-21T23:19:55ZengMDPI AGMicroorganisms2076-26072021-06-0196125010.3390/microorganisms9061250Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and MiceTey Putita Ou0Heidi Auerswald1Saraden In2Borin Peng3Senglong Pang4Sébastien Boyer5Rithy Choeung6Myrielle Dupont-Rouzeyrol7Philippe Dussart8Veasna Duong9Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaMedical Entomology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaURE Dengue and Arboviruses, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Nouméa 98800, New CaledoniaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaVirology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh 12156, CambodiaSince the epidemic in 2007, studies on vector competence for Zika virus (ZIKV) have intensified, showing that the transmission efficiency varies depending on the vector population, ZIKV strain, and dose of the infectious blood meal. In this study, we aimed to investigate the replication of African and Asian ZIKV strains in vitro and in vivo in order to reveal their phenotypic differences. In addition, we investigated the vector competence of Cambodian <i>Aedes aegypti</i> (<i>Ae. aegypti</i>) mosquitoes (urban and rural) for these ZIKV strains. We observed a significantly higher pathogenicity of the African ZIKV strain in vitro (in mosquito and mammalian cells), and in vivo in both <i>Ae. aegypti</i> and mice. Both mosquito populations were competent to transmit ZIKV as early as 7 days p.i., depending on the population and the ZIKV strain. <i>Ae. aegypti</i> from rural habitats showed significant higher transmission and survival rates than those from urban. We observed the highest transmission efficiency for the African ZIKV isolate (93.3% 14 days p.i.) and for the Cambodian ZIKV isolate (80% 14 days p.i.). Overall, our results highlight the phenotypic differences of the ZIKV lineages and the potential risk of ZIKV transmission by <i>Ae. aegypti</i> mosquitoes. Further investigations of Cambodian mosquito species and ZIKV specific surveillance in humans is necessary in order to improve the local risk assessment.https://www.mdpi.com/2076-2607/9/6/1250Zika virusAfrican lineageAsian lineagevector competenceneonatal mouse infection |
spellingShingle | Tey Putita Ou Heidi Auerswald Saraden In Borin Peng Senglong Pang Sébastien Boyer Rithy Choeung Myrielle Dupont-Rouzeyrol Philippe Dussart Veasna Duong Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice Microorganisms Zika virus African lineage Asian lineage vector competence neonatal mouse infection |
title | Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice |
title_full | Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice |
title_fullStr | Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice |
title_full_unstemmed | Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice |
title_short | Replication Variance of African and Asian Lineage Zika Virus Strains in Different Cell Lines, Mosquitoes and Mice |
title_sort | replication variance of african and asian lineage zika virus strains in different cell lines mosquitoes and mice |
topic | Zika virus African lineage Asian lineage vector competence neonatal mouse infection |
url | https://www.mdpi.com/2076-2607/9/6/1250 |
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