Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis
The biological activities of the primary metabolites and secondary metabolites of 69 green cabbage varieties were tested. The LC-MS detection method was used to determine the content of 19 free amino acids (lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine, valine, argini...
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/17/5355 |
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| author | Ning Jin Li Jin Shilei Luo Zhongqi Tang Zeci Liu Shouhui Wei Fanhong Liu Xiaoqiang Zhao Jihua Yu Yuan Zhong |
| author_facet | Ning Jin Li Jin Shilei Luo Zhongqi Tang Zeci Liu Shouhui Wei Fanhong Liu Xiaoqiang Zhao Jihua Yu Yuan Zhong |
| author_sort | Ning Jin |
| collection | DOAJ |
| description | The biological activities of the primary metabolites and secondary metabolites of 69 green cabbage varieties were tested. The LC-MS detection method was used to determine the content of 19 free amino acids (lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine, valine, arginine, asparagine, glycine, proline, tyrosine, glutamine, alanine, aspartic acid, serine, and glutamate). The content of 10 polyphenols (chlorogenic acid, gallic acid, 4-coumaric acid, ferulic acid, gentisic acid, cymarin, erucic acid, benzoic acid, rutin, and kaempferol) was determined by the HPLC detection method. Considering the complexity of the data obtained, variance analysis, diversity analysis, correlation analysis, hierarchical cluster analysis (HCA), and principal component analysis (PCA) were used to process and correlate amino acid or polyphenol data, respectively. The results showed that there were significant differences between the different amino acids and polyphenols of the 69 cabbage varieties. The most abundant amino acids and polyphenols were Glu and rutin, respectively. Both amino acids and polyphenols had a high genetic diversity, and multiple groups of significant or extremely significant correlations. The 69 cabbage varieties were divided into two groups, according to 19 amino acid indexes, by PCA. Among them, seven varieties with high amino acid content all fell into the fourth quadrant. The HCA of amino acids also supports this view. Based on 10 polyphenols, the 69 cabbage varieties were divided into two groups by HCA. Based on 29 indexes of amino acids and polyphenols, 69 cabbage varieties were evaluated and ranked by PCA. Therefore, in this study, cabbage varieties were classified in accordance with the level of amino acids and polyphenols, which provided a theoretical basis for the genetic improvement of nutritional quality in cabbage. |
| first_indexed | 2024-03-10T08:05:44Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:05:44Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3d3f2f2fa5094edf85b19100bfa0338a2023-11-22T11:02:54ZengMDPI AGMolecules1420-30492021-09-012617535510.3390/molecules26175355Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical AnalysisNing Jin0Li Jin1Shilei Luo2Zhongqi Tang3Zeci Liu4Shouhui Wei5Fanhong Liu6Xiaoqiang Zhao7Jihua Yu8Yuan Zhong9College of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaThe biological activities of the primary metabolites and secondary metabolites of 69 green cabbage varieties were tested. The LC-MS detection method was used to determine the content of 19 free amino acids (lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine, valine, arginine, asparagine, glycine, proline, tyrosine, glutamine, alanine, aspartic acid, serine, and glutamate). The content of 10 polyphenols (chlorogenic acid, gallic acid, 4-coumaric acid, ferulic acid, gentisic acid, cymarin, erucic acid, benzoic acid, rutin, and kaempferol) was determined by the HPLC detection method. Considering the complexity of the data obtained, variance analysis, diversity analysis, correlation analysis, hierarchical cluster analysis (HCA), and principal component analysis (PCA) were used to process and correlate amino acid or polyphenol data, respectively. The results showed that there were significant differences between the different amino acids and polyphenols of the 69 cabbage varieties. The most abundant amino acids and polyphenols were Glu and rutin, respectively. Both amino acids and polyphenols had a high genetic diversity, and multiple groups of significant or extremely significant correlations. The 69 cabbage varieties were divided into two groups, according to 19 amino acid indexes, by PCA. Among them, seven varieties with high amino acid content all fell into the fourth quadrant. The HCA of amino acids also supports this view. Based on 10 polyphenols, the 69 cabbage varieties were divided into two groups by HCA. Based on 29 indexes of amino acids and polyphenols, 69 cabbage varieties were evaluated and ranked by PCA. Therefore, in this study, cabbage varieties were classified in accordance with the level of amino acids and polyphenols, which provided a theoretical basis for the genetic improvement of nutritional quality in cabbage.https://www.mdpi.com/1420-3049/26/17/5355cabbage varietiesfree amino acidspolyphenolsHCAPCA |
| spellingShingle | Ning Jin Li Jin Shilei Luo Zhongqi Tang Zeci Liu Shouhui Wei Fanhong Liu Xiaoqiang Zhao Jihua Yu Yuan Zhong Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis Molecules cabbage varieties free amino acids polyphenols HCA PCA |
| title | Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis |
| title_full | Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis |
| title_fullStr | Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis |
| title_full_unstemmed | Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis |
| title_short | Comprehensive Evaluation of Amino Acids and Polyphenols in 69 Varieties of Green Cabbage (<i>Brassica oleracea</i> L. var. <i>capitata</i> L.) Based on Multivariate Statistical Analysis |
| title_sort | comprehensive evaluation of amino acids and polyphenols in 69 varieties of green cabbage i brassica oleracea i l var i capitata i l based on multivariate statistical analysis |
| topic | cabbage varieties free amino acids polyphenols HCA PCA |
| url | https://www.mdpi.com/1420-3049/26/17/5355 |
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