Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III
This paper reports the chromatographic properties of affinity resins in respect of protein antithrombin III. The capacity of resin Toyopearl AF-Heparin was found to be higher than that of resin of Heparin-Sepharose FF. Sorption was carried out by the batch method at room temperature and constant sti...
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2010-04-01
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| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/1000 |
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| author | М. А. Аzhigirova T. L. Dereza I. S. Кryazhevskikh N. V. Sergeeva V. I. Shvets |
| author_facet | М. А. Аzhigirova T. L. Dereza I. S. Кryazhevskikh N. V. Sergeeva V. I. Shvets |
| author_sort | М. А. Аzhigirova |
| collection | DOAJ |
| description | This paper reports the chromatographic properties of affinity resins in respect of protein antithrombin III. The capacity of resin Toyopearl AF-Heparin was found to be higher than that of resin of Heparin-Sepharose FF. Sorption was carried out by the batch method at room temperature and constant stirring. The specific activity of antithrombin III was estimated by the amidolytic method with the use of a chromogenic substrate. The obtained results can be used for the development of column chromatographic method of isolation of antithrombin III from human plasma. |
| first_indexed | 2024-04-10T03:31:46Z |
| format | Article |
| id | doaj.art-3d427ca1b39d4e52b8541f89be766faf |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2024-04-10T03:31:46Z |
| publishDate | 2010-04-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-3d427ca1b39d4e52b8541f89be766faf2023-03-13T07:25:30ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752010-04-01526468994Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin IIIМ. А. Аzhigirova0T. L. Dereza1I. S. Кryazhevskikh2N. V. Sergeeva3V. I. Shvets4Гематологический научный центр РАМНГематологический научный центр РАМНM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Гематологический научный центр РАМНM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571This paper reports the chromatographic properties of affinity resins in respect of protein antithrombin III. The capacity of resin Toyopearl AF-Heparin was found to be higher than that of resin of Heparin-Sepharose FF. Sorption was carried out by the batch method at room temperature and constant stirring. The specific activity of antithrombin III was estimated by the amidolytic method with the use of a chromogenic substrate. The obtained results can be used for the development of column chromatographic method of isolation of antithrombin III from human plasma.https://www.finechem-mirea.ru/jour/article/view/1000antithrombin iiichromatographic resinsproteins of human plasma |
| spellingShingle | М. А. Аzhigirova T. L. Dereza I. S. Кryazhevskikh N. V. Sergeeva V. I. Shvets Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III Тонкие химические технологии antithrombin iii chromatographic resins proteins of human plasma |
| title | Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III |
| title_full | Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III |
| title_fullStr | Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III |
| title_full_unstemmed | Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III |
| title_short | Comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin III |
| title_sort | comparative study of chromatographic properties of affinity resins in respect of protein of antithrombin iii |
| topic | antithrombin iii chromatographic resins proteins of human plasma |
| url | https://www.finechem-mirea.ru/jour/article/view/1000 |
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