Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
<i>Background and Objectives</i>: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. <i>SIRT</i>s are a group of histone deacetylases (HDA...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1648-9144/58/5/653 |
| Summary: | <i>Background and Objectives</i>: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. <i>SIRT</i>s are a group of histone deacetylases (HDACs) intimately involved in cell metabolism and are responsible for altering and regulating numerous cell functions. Understanding <i>SIRT</i>s and their functions in periodontitis may be useful for therapeutic treatment strategies in the future. The aim of our study was to investigate the associations amid <i>SIRT1</i> single-gene nucleotide polymorphisms (rs3818292, rs3758391, and rs7895833) and <i>SIRT1</i> serum levels for patients affected by periodontitis in the Caucasian population. <i>Materials and Methods</i>: The study included 201 patients affected by periodontitis and 500 healthy controls. DNA extraction from peripheral leukocytes was carried out using commercial kits. The real-time PCR method was selected for the determination of the genotype of the periodontitis patients and the control group. The ELISA method was used to measure the <i>SIRT1</i> concentration. A statistical data analysis was performed using “BM SPSS Statistics 27.0” software. <i>Results</i>: The <i>SIRT1</i> rs3818292 AG genotype was associated with a 2-fold and 1.9-fold increase in the development of periodontitis under the codominant and overdominant models (OR = 1.959; CI = 1.239–3.098; <i>p</i> = 0.004; and OR = 1.944; CI = 1.230–3.073; <i>p</i> = 0.004, respectively). The serum <i>SIRT1</i> levels were not statistically significantly different between subjects in the periodontitis and control groups (0.984 (5.159) ng/mL vs. 0.514 (7.705) ng/mL, <i>p</i> = 0.792). <i>Conclusions</i>: in our study, the genotypes and alleles of <i>SIRT1</i> rs3818292, rs3758391, and rs7895833 statistically significantly differed between the periodontitis and control groups, exclusively in the male population and subjects older than 60 years. |
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| ISSN: | 1010-660X 1648-9144 |