Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients
<i>Background and Objectives</i>: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. <i>SIRT</i>s are a group of histone deacetylases (HDA...
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MDPI AG
2022-05-01
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| author | Albertas Kriaučiūnas Rasa Liutkevičienė Greta Gedvilaitė Kristė Kaikarytė Alvita Vilkevičiūtė Darius Gleiznys Ingrida Pacauskienė Gediminas Žekonis |
| author_facet | Albertas Kriaučiūnas Rasa Liutkevičienė Greta Gedvilaitė Kristė Kaikarytė Alvita Vilkevičiūtė Darius Gleiznys Ingrida Pacauskienė Gediminas Žekonis |
| author_sort | Albertas Kriaučiūnas |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. <i>SIRT</i>s are a group of histone deacetylases (HDACs) intimately involved in cell metabolism and are responsible for altering and regulating numerous cell functions. Understanding <i>SIRT</i>s and their functions in periodontitis may be useful for therapeutic treatment strategies in the future. The aim of our study was to investigate the associations amid <i>SIRT1</i> single-gene nucleotide polymorphisms (rs3818292, rs3758391, and rs7895833) and <i>SIRT1</i> serum levels for patients affected by periodontitis in the Caucasian population. <i>Materials and Methods</i>: The study included 201 patients affected by periodontitis and 500 healthy controls. DNA extraction from peripheral leukocytes was carried out using commercial kits. The real-time PCR method was selected for the determination of the genotype of the periodontitis patients and the control group. The ELISA method was used to measure the <i>SIRT1</i> concentration. A statistical data analysis was performed using “BM SPSS Statistics 27.0” software. <i>Results</i>: The <i>SIRT1</i> rs3818292 AG genotype was associated with a 2-fold and 1.9-fold increase in the development of periodontitis under the codominant and overdominant models (OR = 1.959; CI = 1.239–3.098; <i>p</i> = 0.004; and OR = 1.944; CI = 1.230–3.073; <i>p</i> = 0.004, respectively). The serum <i>SIRT1</i> levels were not statistically significantly different between subjects in the periodontitis and control groups (0.984 (5.159) ng/mL vs. 0.514 (7.705) ng/mL, <i>p</i> = 0.792). <i>Conclusions</i>: in our study, the genotypes and alleles of <i>SIRT1</i> rs3818292, rs3758391, and rs7895833 statistically significantly differed between the periodontitis and control groups, exclusively in the male population and subjects older than 60 years. |
| first_indexed | 2024-03-10T03:27:47Z |
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| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-10T03:27:47Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-3d44cb7a3a5f47528f475410259658e52023-11-23T12:04:00ZengMDPI AGMedicina1010-660X1648-91442022-05-0158565310.3390/medicina58050653Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis PatientsAlbertas Kriaučiūnas0Rasa Liutkevičienė1Greta Gedvilaitė2Kristė Kaikarytė3Alvita Vilkevičiūtė4Darius Gleiznys5Ingrida Pacauskienė6Gediminas Žekonis7Department of Prosthodontics, Lithuanian University of Health Sciences, Sukilėlių Str. 51, LT-50106 Kaunas, LithuaniaInstitute of Neuroscience, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaInstitute of Neuroscience, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaInstitute of Neuroscience, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaInstitute of Neuroscience, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaDepartment of Prosthodontics, Lithuanian University of Health Sciences, Sukilėlių Str. 51, LT-50106 Kaunas, LithuaniaDepartment of Dental and Oral Pathology, Lithuanian University of Health Sciences, Eivenių Str. 2, LT-50009 Kaunas, LithuaniaDepartment of Prosthodontics, Lithuanian University of Health Sciences, Sukilėlių Str. 51, LT-50106 Kaunas, Lithuania<i>Background and Objectives</i>: Periodontitis is a multifactorial inflammatory disease associated with biofilm dysbiosis and is defined by progressive periodontium destruction. Genes largely regulate this entire process. <i>SIRT</i>s are a group of histone deacetylases (HDACs) intimately involved in cell metabolism and are responsible for altering and regulating numerous cell functions. Understanding <i>SIRT</i>s and their functions in periodontitis may be useful for therapeutic treatment strategies in the future. The aim of our study was to investigate the associations amid <i>SIRT1</i> single-gene nucleotide polymorphisms (rs3818292, rs3758391, and rs7895833) and <i>SIRT1</i> serum levels for patients affected by periodontitis in the Caucasian population. <i>Materials and Methods</i>: The study included 201 patients affected by periodontitis and 500 healthy controls. DNA extraction from peripheral leukocytes was carried out using commercial kits. The real-time PCR method was selected for the determination of the genotype of the periodontitis patients and the control group. The ELISA method was used to measure the <i>SIRT1</i> concentration. A statistical data analysis was performed using “BM SPSS Statistics 27.0” software. <i>Results</i>: The <i>SIRT1</i> rs3818292 AG genotype was associated with a 2-fold and 1.9-fold increase in the development of periodontitis under the codominant and overdominant models (OR = 1.959; CI = 1.239–3.098; <i>p</i> = 0.004; and OR = 1.944; CI = 1.230–3.073; <i>p</i> = 0.004, respectively). The serum <i>SIRT1</i> levels were not statistically significantly different between subjects in the periodontitis and control groups (0.984 (5.159) ng/mL vs. 0.514 (7.705) ng/mL, <i>p</i> = 0.792). <i>Conclusions</i>: in our study, the genotypes and alleles of <i>SIRT1</i> rs3818292, rs3758391, and rs7895833 statistically significantly differed between the periodontitis and control groups, exclusively in the male population and subjects older than 60 years.https://www.mdpi.com/1648-9144/58/5/653periodontitis<i>SIRT1</i>rs3758391rs3818292rs7895833<i>SIRT1</i> levels |
| spellingShingle | Albertas Kriaučiūnas Rasa Liutkevičienė Greta Gedvilaitė Kristė Kaikarytė Alvita Vilkevičiūtė Darius Gleiznys Ingrida Pacauskienė Gediminas Žekonis Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients Medicina periodontitis <i>SIRT1</i> rs3758391 rs3818292 rs7895833 <i>SIRT1</i> levels |
| title | Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients |
| title_full | Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients |
| title_fullStr | Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients |
| title_full_unstemmed | Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients |
| title_short | Value of Serum Sirtuin-1 (SIRT1) Levels and SIRT1 Gene Variants in Periodontitis Patients |
| title_sort | value of serum sirtuin 1 sirt1 levels and sirt1 gene variants in periodontitis patients |
| topic | periodontitis <i>SIRT1</i> rs3758391 rs3818292 rs7895833 <i>SIRT1</i> levels |
| url | https://www.mdpi.com/1648-9144/58/5/653 |
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