Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies
Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (u...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2003-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200021 |
| _version_ | 1827879841433649152 |
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| author | Brazil Beatriz Gomes Lino-Neto José Brazil Reginaldo Peçanha |
| author_facet | Brazil Beatriz Gomes Lino-Neto José Brazil Reginaldo Peçanha |
| author_sort | Brazil Beatriz Gomes |
| collection | DOAJ |
| description | Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three µm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue. |
| first_indexed | 2024-03-12T18:12:09Z |
| format | Article |
| id | doaj.art-3d44dbe8339240c2b2b1030c957efc17 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:12:09Z |
| publishDate | 2003-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-3d44dbe8339240c2b2b1030c957efc172023-08-02T09:15:08ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602003-01-01982287290Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studiesBrazil Beatriz GomesLino-Neto JoséBrazil Reginaldo PeçanhaPhlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three µm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200021Phlebotominaehistologyhistoresin preparations |
| spellingShingle | Brazil Beatriz Gomes Lino-Neto José Brazil Reginaldo Peçanha Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies Memorias do Instituto Oswaldo Cruz Phlebotominae histology historesin preparations |
| title | Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies |
| title_full | Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies |
| title_fullStr | Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies |
| title_full_unstemmed | Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies |
| title_short | Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies |
| title_sort | preparation of sand fly diptera psychodidae phlebotominae specimens for histological studies |
| topic | Phlebotominae histology historesin preparations |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200021 |
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